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Coi1 is a novel assembly factor of the yeast complex III–complex IV supercomplex
The yeast bc1 complex (complex III) and cytochrome oxidase (complex IV) are mosaics of core subunits encoded by the mitochondrial genome and additional nuclear-encoded proteins imported from the cytosol. Both complexes build various supramolecular assemblies in the mitochondrial inner membrane. The...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5620370/ https://www.ncbi.nlm.nih.gov/pubmed/28794267 http://dx.doi.org/10.1091/mbc.E17-02-0093 |
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author | Singhal, Ravi K. Kruse, Christine Heidler, Juliana Strecker, Valentina Zwicker, Klaus Düsterwald, Lea Westermann, Benedikt Herrmann, Johannes M. Wittig, Ilka Rapaport, Doron |
author_facet | Singhal, Ravi K. Kruse, Christine Heidler, Juliana Strecker, Valentina Zwicker, Klaus Düsterwald, Lea Westermann, Benedikt Herrmann, Johannes M. Wittig, Ilka Rapaport, Doron |
author_sort | Singhal, Ravi K. |
collection | PubMed |
description | The yeast bc1 complex (complex III) and cytochrome oxidase (complex IV) are mosaics of core subunits encoded by the mitochondrial genome and additional nuclear-encoded proteins imported from the cytosol. Both complexes build various supramolecular assemblies in the mitochondrial inner membrane. The formation of the individual complexes and their supercomplexes depends on the activity of dedicated assembly factors. We identified a so far uncharacterized mitochondrial protein (open reading frame YDR381C-A) as an important assembly factor for complex III, complex IV, and their supercomplexes. Therefore we named this protein Cox interacting (Coi) 1. Deletion of COI1 results in decreased respiratory growth, reduced membrane potential, and hampered respiration, as well as slow fermentative growth at low temperature. In addition, coi1Δ cells harbor reduced steady-state levels of subunits of complexes III and IV and of the assembled complexes and supercomplexes. Interaction of Coi1 with respiratory chain subunits seems transient, as it appears to be a stoichiometric subunit neither of complex III nor of complex IV. Collectively this work identifies a novel protein that plays a role in the assembly of the mitochondrial respiratory chain. |
format | Online Article Text |
id | pubmed-5620370 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-56203702017-12-16 Coi1 is a novel assembly factor of the yeast complex III–complex IV supercomplex Singhal, Ravi K. Kruse, Christine Heidler, Juliana Strecker, Valentina Zwicker, Klaus Düsterwald, Lea Westermann, Benedikt Herrmann, Johannes M. Wittig, Ilka Rapaport, Doron Mol Biol Cell Articles The yeast bc1 complex (complex III) and cytochrome oxidase (complex IV) are mosaics of core subunits encoded by the mitochondrial genome and additional nuclear-encoded proteins imported from the cytosol. Both complexes build various supramolecular assemblies in the mitochondrial inner membrane. The formation of the individual complexes and their supercomplexes depends on the activity of dedicated assembly factors. We identified a so far uncharacterized mitochondrial protein (open reading frame YDR381C-A) as an important assembly factor for complex III, complex IV, and their supercomplexes. Therefore we named this protein Cox interacting (Coi) 1. Deletion of COI1 results in decreased respiratory growth, reduced membrane potential, and hampered respiration, as well as slow fermentative growth at low temperature. In addition, coi1Δ cells harbor reduced steady-state levels of subunits of complexes III and IV and of the assembled complexes and supercomplexes. Interaction of Coi1 with respiratory chain subunits seems transient, as it appears to be a stoichiometric subunit neither of complex III nor of complex IV. Collectively this work identifies a novel protein that plays a role in the assembly of the mitochondrial respiratory chain. The American Society for Cell Biology 2017-10-01 /pmc/articles/PMC5620370/ /pubmed/28794267 http://dx.doi.org/10.1091/mbc.E17-02-0093 Text en © 2017 Singhal et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. |
spellingShingle | Articles Singhal, Ravi K. Kruse, Christine Heidler, Juliana Strecker, Valentina Zwicker, Klaus Düsterwald, Lea Westermann, Benedikt Herrmann, Johannes M. Wittig, Ilka Rapaport, Doron Coi1 is a novel assembly factor of the yeast complex III–complex IV supercomplex |
title | Coi1 is a novel assembly factor of the yeast complex III–complex IV supercomplex |
title_full | Coi1 is a novel assembly factor of the yeast complex III–complex IV supercomplex |
title_fullStr | Coi1 is a novel assembly factor of the yeast complex III–complex IV supercomplex |
title_full_unstemmed | Coi1 is a novel assembly factor of the yeast complex III–complex IV supercomplex |
title_short | Coi1 is a novel assembly factor of the yeast complex III–complex IV supercomplex |
title_sort | coi1 is a novel assembly factor of the yeast complex iii–complex iv supercomplex |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5620370/ https://www.ncbi.nlm.nih.gov/pubmed/28794267 http://dx.doi.org/10.1091/mbc.E17-02-0093 |
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