Cargando…

Coi1 is a novel assembly factor of the yeast complex III–complex IV supercomplex

The yeast bc1 complex (complex III) and cytochrome oxidase (complex IV) are mosaics of core subunits encoded by the mitochondrial genome and additional nuclear-encoded proteins imported from the cytosol. Both complexes build various supramolecular assemblies in the mitochondrial inner membrane. The...

Descripción completa

Detalles Bibliográficos
Autores principales: Singhal, Ravi K., Kruse, Christine, Heidler, Juliana, Strecker, Valentina, Zwicker, Klaus, Düsterwald, Lea, Westermann, Benedikt, Herrmann, Johannes M., Wittig, Ilka, Rapaport, Doron
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5620370/
https://www.ncbi.nlm.nih.gov/pubmed/28794267
http://dx.doi.org/10.1091/mbc.E17-02-0093
_version_ 1783267572438794240
author Singhal, Ravi K.
Kruse, Christine
Heidler, Juliana
Strecker, Valentina
Zwicker, Klaus
Düsterwald, Lea
Westermann, Benedikt
Herrmann, Johannes M.
Wittig, Ilka
Rapaport, Doron
author_facet Singhal, Ravi K.
Kruse, Christine
Heidler, Juliana
Strecker, Valentina
Zwicker, Klaus
Düsterwald, Lea
Westermann, Benedikt
Herrmann, Johannes M.
Wittig, Ilka
Rapaport, Doron
author_sort Singhal, Ravi K.
collection PubMed
description The yeast bc1 complex (complex III) and cytochrome oxidase (complex IV) are mosaics of core subunits encoded by the mitochondrial genome and additional nuclear-encoded proteins imported from the cytosol. Both complexes build various supramolecular assemblies in the mitochondrial inner membrane. The formation of the individual complexes and their supercomplexes depends on the activity of dedicated assembly factors. We identified a so far uncharacterized mitochondrial protein (open reading frame YDR381C-A) as an important assembly factor for complex III, complex IV, and their supercomplexes. Therefore we named this protein Cox interacting (Coi) 1. Deletion of COI1 results in decreased respiratory growth, reduced membrane potential, and hampered respiration, as well as slow fermentative growth at low temperature. In addition, coi1Δ cells harbor reduced steady-state levels of subunits of complexes III and IV and of the assembled complexes and supercomplexes. Interaction of Coi1 with respiratory chain subunits seems transient, as it appears to be a stoichiometric subunit neither of complex III nor of complex IV. Collectively this work identifies a novel protein that plays a role in the assembly of the mitochondrial respiratory chain.
format Online
Article
Text
id pubmed-5620370
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher The American Society for Cell Biology
record_format MEDLINE/PubMed
spelling pubmed-56203702017-12-16 Coi1 is a novel assembly factor of the yeast complex III–complex IV supercomplex Singhal, Ravi K. Kruse, Christine Heidler, Juliana Strecker, Valentina Zwicker, Klaus Düsterwald, Lea Westermann, Benedikt Herrmann, Johannes M. Wittig, Ilka Rapaport, Doron Mol Biol Cell Articles The yeast bc1 complex (complex III) and cytochrome oxidase (complex IV) are mosaics of core subunits encoded by the mitochondrial genome and additional nuclear-encoded proteins imported from the cytosol. Both complexes build various supramolecular assemblies in the mitochondrial inner membrane. The formation of the individual complexes and their supercomplexes depends on the activity of dedicated assembly factors. We identified a so far uncharacterized mitochondrial protein (open reading frame YDR381C-A) as an important assembly factor for complex III, complex IV, and their supercomplexes. Therefore we named this protein Cox interacting (Coi) 1. Deletion of COI1 results in decreased respiratory growth, reduced membrane potential, and hampered respiration, as well as slow fermentative growth at low temperature. In addition, coi1Δ cells harbor reduced steady-state levels of subunits of complexes III and IV and of the assembled complexes and supercomplexes. Interaction of Coi1 with respiratory chain subunits seems transient, as it appears to be a stoichiometric subunit neither of complex III nor of complex IV. Collectively this work identifies a novel protein that plays a role in the assembly of the mitochondrial respiratory chain. The American Society for Cell Biology 2017-10-01 /pmc/articles/PMC5620370/ /pubmed/28794267 http://dx.doi.org/10.1091/mbc.E17-02-0093 Text en © 2017 Singhal et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.
spellingShingle Articles
Singhal, Ravi K.
Kruse, Christine
Heidler, Juliana
Strecker, Valentina
Zwicker, Klaus
Düsterwald, Lea
Westermann, Benedikt
Herrmann, Johannes M.
Wittig, Ilka
Rapaport, Doron
Coi1 is a novel assembly factor of the yeast complex III–complex IV supercomplex
title Coi1 is a novel assembly factor of the yeast complex III–complex IV supercomplex
title_full Coi1 is a novel assembly factor of the yeast complex III–complex IV supercomplex
title_fullStr Coi1 is a novel assembly factor of the yeast complex III–complex IV supercomplex
title_full_unstemmed Coi1 is a novel assembly factor of the yeast complex III–complex IV supercomplex
title_short Coi1 is a novel assembly factor of the yeast complex III–complex IV supercomplex
title_sort coi1 is a novel assembly factor of the yeast complex iii–complex iv supercomplex
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5620370/
https://www.ncbi.nlm.nih.gov/pubmed/28794267
http://dx.doi.org/10.1091/mbc.E17-02-0093
work_keys_str_mv AT singhalravik coi1isanovelassemblyfactoroftheyeastcomplexiiicomplexivsupercomplex
AT krusechristine coi1isanovelassemblyfactoroftheyeastcomplexiiicomplexivsupercomplex
AT heidlerjuliana coi1isanovelassemblyfactoroftheyeastcomplexiiicomplexivsupercomplex
AT streckervalentina coi1isanovelassemblyfactoroftheyeastcomplexiiicomplexivsupercomplex
AT zwickerklaus coi1isanovelassemblyfactoroftheyeastcomplexiiicomplexivsupercomplex
AT dusterwaldlea coi1isanovelassemblyfactoroftheyeastcomplexiiicomplexivsupercomplex
AT westermannbenedikt coi1isanovelassemblyfactoroftheyeastcomplexiiicomplexivsupercomplex
AT herrmannjohannesm coi1isanovelassemblyfactoroftheyeastcomplexiiicomplexivsupercomplex
AT wittigilka coi1isanovelassemblyfactoroftheyeastcomplexiiicomplexivsupercomplex
AT rapaportdoron coi1isanovelassemblyfactoroftheyeastcomplexiiicomplexivsupercomplex