Cargando…
Crotonis Fructus Extract Inhibits 12-O-Tetradecanoylphorbol-13-Acetate-Induced Expression of Matrix Metalloproteinase-9 via the Activator Protein-1 Pathway in MCF-7 Cells
PURPOSE: Metastatic cancers spread from the primary site of origin to other parts of the body. Matrix metalloproteinase-9 (MMP-9) is essential in metastatic cancers owing to its major role in cancer cell invasion. Crotonis fructus (CF), the mature fruits of Croton tiglium L., have been used for the...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Korean Breast Cancer Society
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5620437/ https://www.ncbi.nlm.nih.gov/pubmed/28970848 http://dx.doi.org/10.4048/jbc.2017.20.3.234 |
_version_ | 1783267586388000768 |
---|---|
author | Song, Hyun-Kyung Lee, Guem-San Park, Sueng Hyuk Noh, Eun-Mi Kim, Jeong-Mi Ryu, Do-Gon Jung, Sung Hoo Youn, Hyun Jo Lee, Young-Rae Kwon, Kang-Beom |
author_facet | Song, Hyun-Kyung Lee, Guem-San Park, Sueng Hyuk Noh, Eun-Mi Kim, Jeong-Mi Ryu, Do-Gon Jung, Sung Hoo Youn, Hyun Jo Lee, Young-Rae Kwon, Kang-Beom |
author_sort | Song, Hyun-Kyung |
collection | PubMed |
description | PURPOSE: Metastatic cancers spread from the primary site of origin to other parts of the body. Matrix metalloproteinase-9 (MMP-9) is essential in metastatic cancers owing to its major role in cancer cell invasion. Crotonis fructus (CF), the mature fruits of Croton tiglium L., have been used for the treatment of gastrointestinal disturbance in Asia. In this study, the effect of the ethanol extract of CF (CFE) on MMP-9 activity and the invasion of 12-O-tetradecanoylphorbol-13-acetate (TPA)-treated MCF-7 cells was examined. METHODS: The cell viability was evaluated using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. The expression of MMP-9 was examined by Western blotting, zymography, and real-time polymerase chain reaction. An electrophoretic mobility gel shift assay was performed to detect activator protein-1 (AP-1) DNA binding activity and cell invasiveness was measured by an in vitro Matrigel invasion assay. RESULTS: CFE significantly suppressed MMP-9 expression and activation in a dose-dependent manner. Furthermore, CFE attenuated the TPA-induced activation of AP-1. CONCLUSION: The results indicated that the inhibitory effects of CFE against TPA-induced MMP-9 expression and MCF-7 cell invasion were dependent on the protein kinase C δ/p38/c-Jun N-terminal kinase/AP-1 pathway. Therefore, CFE could restrict breast cancer invasiveness owing to its ability to inhibit MMP-9 activity. |
format | Online Article Text |
id | pubmed-5620437 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Korean Breast Cancer Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-56204372017-10-02 Crotonis Fructus Extract Inhibits 12-O-Tetradecanoylphorbol-13-Acetate-Induced Expression of Matrix Metalloproteinase-9 via the Activator Protein-1 Pathway in MCF-7 Cells Song, Hyun-Kyung Lee, Guem-San Park, Sueng Hyuk Noh, Eun-Mi Kim, Jeong-Mi Ryu, Do-Gon Jung, Sung Hoo Youn, Hyun Jo Lee, Young-Rae Kwon, Kang-Beom J Breast Cancer Original Article PURPOSE: Metastatic cancers spread from the primary site of origin to other parts of the body. Matrix metalloproteinase-9 (MMP-9) is essential in metastatic cancers owing to its major role in cancer cell invasion. Crotonis fructus (CF), the mature fruits of Croton tiglium L., have been used for the treatment of gastrointestinal disturbance in Asia. In this study, the effect of the ethanol extract of CF (CFE) on MMP-9 activity and the invasion of 12-O-tetradecanoylphorbol-13-acetate (TPA)-treated MCF-7 cells was examined. METHODS: The cell viability was evaluated using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. The expression of MMP-9 was examined by Western blotting, zymography, and real-time polymerase chain reaction. An electrophoretic mobility gel shift assay was performed to detect activator protein-1 (AP-1) DNA binding activity and cell invasiveness was measured by an in vitro Matrigel invasion assay. RESULTS: CFE significantly suppressed MMP-9 expression and activation in a dose-dependent manner. Furthermore, CFE attenuated the TPA-induced activation of AP-1. CONCLUSION: The results indicated that the inhibitory effects of CFE against TPA-induced MMP-9 expression and MCF-7 cell invasion were dependent on the protein kinase C δ/p38/c-Jun N-terminal kinase/AP-1 pathway. Therefore, CFE could restrict breast cancer invasiveness owing to its ability to inhibit MMP-9 activity. Korean Breast Cancer Society 2017-09 2017-09-22 /pmc/articles/PMC5620437/ /pubmed/28970848 http://dx.doi.org/10.4048/jbc.2017.20.3.234 Text en © 2017 Korean Breast Cancer Society http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Song, Hyun-Kyung Lee, Guem-San Park, Sueng Hyuk Noh, Eun-Mi Kim, Jeong-Mi Ryu, Do-Gon Jung, Sung Hoo Youn, Hyun Jo Lee, Young-Rae Kwon, Kang-Beom Crotonis Fructus Extract Inhibits 12-O-Tetradecanoylphorbol-13-Acetate-Induced Expression of Matrix Metalloproteinase-9 via the Activator Protein-1 Pathway in MCF-7 Cells |
title | Crotonis Fructus Extract Inhibits 12-O-Tetradecanoylphorbol-13-Acetate-Induced Expression of Matrix Metalloproteinase-9 via the Activator Protein-1 Pathway in MCF-7 Cells |
title_full | Crotonis Fructus Extract Inhibits 12-O-Tetradecanoylphorbol-13-Acetate-Induced Expression of Matrix Metalloproteinase-9 via the Activator Protein-1 Pathway in MCF-7 Cells |
title_fullStr | Crotonis Fructus Extract Inhibits 12-O-Tetradecanoylphorbol-13-Acetate-Induced Expression of Matrix Metalloproteinase-9 via the Activator Protein-1 Pathway in MCF-7 Cells |
title_full_unstemmed | Crotonis Fructus Extract Inhibits 12-O-Tetradecanoylphorbol-13-Acetate-Induced Expression of Matrix Metalloproteinase-9 via the Activator Protein-1 Pathway in MCF-7 Cells |
title_short | Crotonis Fructus Extract Inhibits 12-O-Tetradecanoylphorbol-13-Acetate-Induced Expression of Matrix Metalloproteinase-9 via the Activator Protein-1 Pathway in MCF-7 Cells |
title_sort | crotonis fructus extract inhibits 12-o-tetradecanoylphorbol-13-acetate-induced expression of matrix metalloproteinase-9 via the activator protein-1 pathway in mcf-7 cells |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5620437/ https://www.ncbi.nlm.nih.gov/pubmed/28970848 http://dx.doi.org/10.4048/jbc.2017.20.3.234 |
work_keys_str_mv | AT songhyunkyung crotonisfructusextractinhibits12otetradecanoylphorbol13acetateinducedexpressionofmatrixmetalloproteinase9viatheactivatorprotein1pathwayinmcf7cells AT leeguemsan crotonisfructusextractinhibits12otetradecanoylphorbol13acetateinducedexpressionofmatrixmetalloproteinase9viatheactivatorprotein1pathwayinmcf7cells AT parksuenghyuk crotonisfructusextractinhibits12otetradecanoylphorbol13acetateinducedexpressionofmatrixmetalloproteinase9viatheactivatorprotein1pathwayinmcf7cells AT noheunmi crotonisfructusextractinhibits12otetradecanoylphorbol13acetateinducedexpressionofmatrixmetalloproteinase9viatheactivatorprotein1pathwayinmcf7cells AT kimjeongmi crotonisfructusextractinhibits12otetradecanoylphorbol13acetateinducedexpressionofmatrixmetalloproteinase9viatheactivatorprotein1pathwayinmcf7cells AT ryudogon crotonisfructusextractinhibits12otetradecanoylphorbol13acetateinducedexpressionofmatrixmetalloproteinase9viatheactivatorprotein1pathwayinmcf7cells AT jungsunghoo crotonisfructusextractinhibits12otetradecanoylphorbol13acetateinducedexpressionofmatrixmetalloproteinase9viatheactivatorprotein1pathwayinmcf7cells AT younhyunjo crotonisfructusextractinhibits12otetradecanoylphorbol13acetateinducedexpressionofmatrixmetalloproteinase9viatheactivatorprotein1pathwayinmcf7cells AT leeyoungrae crotonisfructusextractinhibits12otetradecanoylphorbol13acetateinducedexpressionofmatrixmetalloproteinase9viatheactivatorprotein1pathwayinmcf7cells AT kwonkangbeom crotonisfructusextractinhibits12otetradecanoylphorbol13acetateinducedexpressionofmatrixmetalloproteinase9viatheactivatorprotein1pathwayinmcf7cells |