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A Novel Organic Electrochemical Transistor-Based Platform for Monitoring the Senescent Green Vegetative Phase of Haematococcus pluvialis Cells

The freshwater unicellular microalga Haematococcus pluvialis (H. pluvialis) has gained increasing attention because of its high-value metabolite astaxanthin, a super anti-oxidant. For the maximum astaxanthin production, a key problem is how to determine the senescent green vegetative phase of H. plu...

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Autores principales: Wei, Weiwei, Xiao, Kang, Tao, Ming, Nie, Lifu, Liu, Dan, Ke, Shanming, Zeng, Xierong, Hu, Zhangli, Lin, Peng, Zhang, Yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5620665/
https://www.ncbi.nlm.nih.gov/pubmed/28858233
http://dx.doi.org/10.3390/s17091997
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author Wei, Weiwei
Xiao, Kang
Tao, Ming
Nie, Lifu
Liu, Dan
Ke, Shanming
Zeng, Xierong
Hu, Zhangli
Lin, Peng
Zhang, Yu
author_facet Wei, Weiwei
Xiao, Kang
Tao, Ming
Nie, Lifu
Liu, Dan
Ke, Shanming
Zeng, Xierong
Hu, Zhangli
Lin, Peng
Zhang, Yu
author_sort Wei, Weiwei
collection PubMed
description The freshwater unicellular microalga Haematococcus pluvialis (H. pluvialis) has gained increasing attention because of its high-value metabolite astaxanthin, a super anti-oxidant. For the maximum astaxanthin production, a key problem is how to determine the senescent green vegetative phase of H. pluvialis cells to apply the astaxanthin production inducers. The conventional methods are time-consuming and laborious. In this study, a novel platform based on organic electrochemical transistor (OECT) was produced. A significant channel current change of OECTs caused by settled H. pluvialis cells on the poly(3,4-ethylenedioxythiophene): polystyrene sulfonate (PEDOT: PSS) film was recorded commencing from 75 min and a stationary stage was achieved at 120 min after the combined treatment of blue light irradiation and sodium bicarbonate solution additives, which indicate the onset and maturation of the senescent green vegetative phase, respectively. Therefore, the appropriate time point (120 min after sample loading) to apply astaxanthin production inducers was determined by as-fabricated OECTs. This work may assist to develop a real-time biosensor to indicate the appropriate time to apply inducers for a maximum astaxanthin production of H. pluvialis cells.
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spelling pubmed-56206652017-10-03 A Novel Organic Electrochemical Transistor-Based Platform for Monitoring the Senescent Green Vegetative Phase of Haematococcus pluvialis Cells Wei, Weiwei Xiao, Kang Tao, Ming Nie, Lifu Liu, Dan Ke, Shanming Zeng, Xierong Hu, Zhangli Lin, Peng Zhang, Yu Sensors (Basel) Article The freshwater unicellular microalga Haematococcus pluvialis (H. pluvialis) has gained increasing attention because of its high-value metabolite astaxanthin, a super anti-oxidant. For the maximum astaxanthin production, a key problem is how to determine the senescent green vegetative phase of H. pluvialis cells to apply the astaxanthin production inducers. The conventional methods are time-consuming and laborious. In this study, a novel platform based on organic electrochemical transistor (OECT) was produced. A significant channel current change of OECTs caused by settled H. pluvialis cells on the poly(3,4-ethylenedioxythiophene): polystyrene sulfonate (PEDOT: PSS) film was recorded commencing from 75 min and a stationary stage was achieved at 120 min after the combined treatment of blue light irradiation and sodium bicarbonate solution additives, which indicate the onset and maturation of the senescent green vegetative phase, respectively. Therefore, the appropriate time point (120 min after sample loading) to apply astaxanthin production inducers was determined by as-fabricated OECTs. This work may assist to develop a real-time biosensor to indicate the appropriate time to apply inducers for a maximum astaxanthin production of H. pluvialis cells. MDPI 2017-08-31 /pmc/articles/PMC5620665/ /pubmed/28858233 http://dx.doi.org/10.3390/s17091997 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wei, Weiwei
Xiao, Kang
Tao, Ming
Nie, Lifu
Liu, Dan
Ke, Shanming
Zeng, Xierong
Hu, Zhangli
Lin, Peng
Zhang, Yu
A Novel Organic Electrochemical Transistor-Based Platform for Monitoring the Senescent Green Vegetative Phase of Haematococcus pluvialis Cells
title A Novel Organic Electrochemical Transistor-Based Platform for Monitoring the Senescent Green Vegetative Phase of Haematococcus pluvialis Cells
title_full A Novel Organic Electrochemical Transistor-Based Platform for Monitoring the Senescent Green Vegetative Phase of Haematococcus pluvialis Cells
title_fullStr A Novel Organic Electrochemical Transistor-Based Platform for Monitoring the Senescent Green Vegetative Phase of Haematococcus pluvialis Cells
title_full_unstemmed A Novel Organic Electrochemical Transistor-Based Platform for Monitoring the Senescent Green Vegetative Phase of Haematococcus pluvialis Cells
title_short A Novel Organic Electrochemical Transistor-Based Platform for Monitoring the Senescent Green Vegetative Phase of Haematococcus pluvialis Cells
title_sort novel organic electrochemical transistor-based platform for monitoring the senescent green vegetative phase of haematococcus pluvialis cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5620665/
https://www.ncbi.nlm.nih.gov/pubmed/28858233
http://dx.doi.org/10.3390/s17091997
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