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Evaluation of irreversible protein thermal inactivation caused by breakage of disulphide bonds using methanethiosulphonate
Many extracellular globular proteins have evolved to possess disulphide bonds in their native conformations, which aids in thermodynamic stabilisation. However, disulphide bond breakage by heating leads to irreversible protein denaturation through disulphide-thiol exchange reactions. In this study,...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2017
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5622167/ https://www.ncbi.nlm.nih.gov/pubmed/28963503 http://dx.doi.org/10.1038/s41598-017-12748-y |