Cargando…

Flavonolignans Inhibit IL1-β-Induced Cross-Talk between Blood Platelets and Leukocytes

Interleukin-1 beta (IL-1β)—the most potent pro-inflammatory is responsible for a broad spectrum of immune and inflammatory responses, it induces T-cell and B-cell activation and consequently the synthesis of other pro-inflammatory cytokines (such as IFN-γ and TNF). IL-1β induces the formation of blo...

Descripción completa

Detalles Bibliográficos
Autores principales: Bijak, Michal, Dziedzic, Angela, Synowiec, Ewelina, Sliwinski, Tomasz, Saluk-Bijak, Joanna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5622782/
https://www.ncbi.nlm.nih.gov/pubmed/28914761
http://dx.doi.org/10.3390/nu9091022
_version_ 1783267986034917376
author Bijak, Michal
Dziedzic, Angela
Synowiec, Ewelina
Sliwinski, Tomasz
Saluk-Bijak, Joanna
author_facet Bijak, Michal
Dziedzic, Angela
Synowiec, Ewelina
Sliwinski, Tomasz
Saluk-Bijak, Joanna
author_sort Bijak, Michal
collection PubMed
description Interleukin-1 beta (IL-1β)—the most potent pro-inflammatory is responsible for a broad spectrum of immune and inflammatory responses, it induces T-cell and B-cell activation and consequently the synthesis of other pro-inflammatory cytokines (such as IFN-γ and TNF). IL-1β induces the formation of blood platelet-leukocyte aggregates (PLAs), which suggests that IL-1β significantly affects the cross-talk between blood platelets and the immune response system, leading to coronary thrombosis. The aim of our study is to investigate the effect of flavonolignans (silybin, silychristin and silydianin) on the IL-1β-induced interaction between platelets and leukocytes, as well as on the expression and the secretion of pro-inflammatory factors. Whole blood samples were pre-incubated with commercially available flavonolignans (silybin, silychristin and silydianin) in a concentration range of 10–100 µM (30 min, 37 °C). Next, samples were activated by IL-1β for 1 h. Blood platelet-leukocyte aggregates were detected by using the double-labeled flow cytometry (CD61/CD45). The level of produced cytokines was estimated via the ELISA immunoenzymatic method. IFN-γ and TNF gene expression was evaluated using Real Time PCR with TaqMan arrays. We observed that in a dose-dependent manner, silybin and silychristin inhibit the IL-1β-induced formation of blood platelet-leukocyte aggregates in whole blood samples, as well as the production of pro-inflammatory cytokines—IL-2, TNF, INF-α, and INF-γ. Additionally, these two flavonolignans abolished the IL-1β-induced expression of mRNA for IFN-γ and TNF. Our current results demonstrate that flavonolignans can be novel compounds used in the prevention of cardiovascular diseases with dual-use action as antiplatelet and anti-inflammatory agents.
format Online
Article
Text
id pubmed-5622782
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-56227822017-10-05 Flavonolignans Inhibit IL1-β-Induced Cross-Talk between Blood Platelets and Leukocytes Bijak, Michal Dziedzic, Angela Synowiec, Ewelina Sliwinski, Tomasz Saluk-Bijak, Joanna Nutrients Article Interleukin-1 beta (IL-1β)—the most potent pro-inflammatory is responsible for a broad spectrum of immune and inflammatory responses, it induces T-cell and B-cell activation and consequently the synthesis of other pro-inflammatory cytokines (such as IFN-γ and TNF). IL-1β induces the formation of blood platelet-leukocyte aggregates (PLAs), which suggests that IL-1β significantly affects the cross-talk between blood platelets and the immune response system, leading to coronary thrombosis. The aim of our study is to investigate the effect of flavonolignans (silybin, silychristin and silydianin) on the IL-1β-induced interaction between platelets and leukocytes, as well as on the expression and the secretion of pro-inflammatory factors. Whole blood samples were pre-incubated with commercially available flavonolignans (silybin, silychristin and silydianin) in a concentration range of 10–100 µM (30 min, 37 °C). Next, samples were activated by IL-1β for 1 h. Blood platelet-leukocyte aggregates were detected by using the double-labeled flow cytometry (CD61/CD45). The level of produced cytokines was estimated via the ELISA immunoenzymatic method. IFN-γ and TNF gene expression was evaluated using Real Time PCR with TaqMan arrays. We observed that in a dose-dependent manner, silybin and silychristin inhibit the IL-1β-induced formation of blood platelet-leukocyte aggregates in whole blood samples, as well as the production of pro-inflammatory cytokines—IL-2, TNF, INF-α, and INF-γ. Additionally, these two flavonolignans abolished the IL-1β-induced expression of mRNA for IFN-γ and TNF. Our current results demonstrate that flavonolignans can be novel compounds used in the prevention of cardiovascular diseases with dual-use action as antiplatelet and anti-inflammatory agents. MDPI 2017-09-15 /pmc/articles/PMC5622782/ /pubmed/28914761 http://dx.doi.org/10.3390/nu9091022 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bijak, Michal
Dziedzic, Angela
Synowiec, Ewelina
Sliwinski, Tomasz
Saluk-Bijak, Joanna
Flavonolignans Inhibit IL1-β-Induced Cross-Talk between Blood Platelets and Leukocytes
title Flavonolignans Inhibit IL1-β-Induced Cross-Talk between Blood Platelets and Leukocytes
title_full Flavonolignans Inhibit IL1-β-Induced Cross-Talk between Blood Platelets and Leukocytes
title_fullStr Flavonolignans Inhibit IL1-β-Induced Cross-Talk between Blood Platelets and Leukocytes
title_full_unstemmed Flavonolignans Inhibit IL1-β-Induced Cross-Talk between Blood Platelets and Leukocytes
title_short Flavonolignans Inhibit IL1-β-Induced Cross-Talk between Blood Platelets and Leukocytes
title_sort flavonolignans inhibit il1-β-induced cross-talk between blood platelets and leukocytes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5622782/
https://www.ncbi.nlm.nih.gov/pubmed/28914761
http://dx.doi.org/10.3390/nu9091022
work_keys_str_mv AT bijakmichal flavonolignansinhibitil1binducedcrosstalkbetweenbloodplateletsandleukocytes
AT dziedzicangela flavonolignansinhibitil1binducedcrosstalkbetweenbloodplateletsandleukocytes
AT synowiecewelina flavonolignansinhibitil1binducedcrosstalkbetweenbloodplateletsandleukocytes
AT sliwinskitomasz flavonolignansinhibitil1binducedcrosstalkbetweenbloodplateletsandleukocytes
AT salukbijakjoanna flavonolignansinhibitil1binducedcrosstalkbetweenbloodplateletsandleukocytes