Use of RNALater(®) Preservation for Virome Sequencing in Outbreak Settings

Outbreaks of infectious diseases may occur in animal and human populations; this underlines the need for suitable One Health approaches. During outbreak situations, straightforward identification of etiological agents is indispensable for taking countermeasures. A recently published protocol for met...

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Autores principales: Kohl, Claudia, Wegener, Merle, Nitsche, Andreas, Kurth, Andreas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5623189/
https://www.ncbi.nlm.nih.gov/pubmed/29018436
http://dx.doi.org/10.3389/fmicb.2017.01888
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author Kohl, Claudia
Wegener, Merle
Nitsche, Andreas
Kurth, Andreas
author_facet Kohl, Claudia
Wegener, Merle
Nitsche, Andreas
Kurth, Andreas
author_sort Kohl, Claudia
collection PubMed
description Outbreaks of infectious diseases may occur in animal and human populations; this underlines the need for suitable One Health approaches. During outbreak situations, straightforward identification of etiological agents is indispensable for taking countermeasures. A recently published protocol for metagenomic virus detection in clinical specimens (TUViD-VM) was developed for snap-frozen tissues which can be challenging to obtain. Here, we describe the use of RNALater(®)-treated tissue at ambient temperatures for virome sequencing. This study demonstrates that samples stored in RNALater(®) buffer yield similar results to those stored snap-frozen.
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spelling pubmed-56231892017-10-10 Use of RNALater(®) Preservation for Virome Sequencing in Outbreak Settings Kohl, Claudia Wegener, Merle Nitsche, Andreas Kurth, Andreas Front Microbiol Microbiology Outbreaks of infectious diseases may occur in animal and human populations; this underlines the need for suitable One Health approaches. During outbreak situations, straightforward identification of etiological agents is indispensable for taking countermeasures. A recently published protocol for metagenomic virus detection in clinical specimens (TUViD-VM) was developed for snap-frozen tissues which can be challenging to obtain. Here, we describe the use of RNALater(®)-treated tissue at ambient temperatures for virome sequencing. This study demonstrates that samples stored in RNALater(®) buffer yield similar results to those stored snap-frozen. Frontiers Media S.A. 2017-09-26 /pmc/articles/PMC5623189/ /pubmed/29018436 http://dx.doi.org/10.3389/fmicb.2017.01888 Text en Copyright © 2017 Kohl, Wegener, Nitsche and Kurth. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Kohl, Claudia
Wegener, Merle
Nitsche, Andreas
Kurth, Andreas
Use of RNALater(®) Preservation for Virome Sequencing in Outbreak Settings
title Use of RNALater(®) Preservation for Virome Sequencing in Outbreak Settings
title_full Use of RNALater(®) Preservation for Virome Sequencing in Outbreak Settings
title_fullStr Use of RNALater(®) Preservation for Virome Sequencing in Outbreak Settings
title_full_unstemmed Use of RNALater(®) Preservation for Virome Sequencing in Outbreak Settings
title_short Use of RNALater(®) Preservation for Virome Sequencing in Outbreak Settings
title_sort use of rnalater(®) preservation for virome sequencing in outbreak settings
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5623189/
https://www.ncbi.nlm.nih.gov/pubmed/29018436
http://dx.doi.org/10.3389/fmicb.2017.01888
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