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A Survey of the Use of Iterative Reconstruction Algorithms in Electron Microscopy
One of the key steps in Electron Microscopy is the tomographic reconstruction of a three-dimensional (3D) map of the specimen being studied from a set of two-dimensional (2D) projections acquired at the microscope. This tomographic reconstruction may be performed with different reconstruction algori...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5623807/ https://www.ncbi.nlm.nih.gov/pubmed/29312997 http://dx.doi.org/10.1155/2017/6482567 |
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author | Sorzano, C. O. S. Vargas, J. Otón, J. de la Rosa-Trevín, J. M. Vilas, J. L. Kazemi, M. Melero, R. del Caño, L. Cuenca, J. Conesa, P. Gómez-Blanco, J. Marabini, R. Carazo, J. M. |
author_facet | Sorzano, C. O. S. Vargas, J. Otón, J. de la Rosa-Trevín, J. M. Vilas, J. L. Kazemi, M. Melero, R. del Caño, L. Cuenca, J. Conesa, P. Gómez-Blanco, J. Marabini, R. Carazo, J. M. |
author_sort | Sorzano, C. O. S. |
collection | PubMed |
description | One of the key steps in Electron Microscopy is the tomographic reconstruction of a three-dimensional (3D) map of the specimen being studied from a set of two-dimensional (2D) projections acquired at the microscope. This tomographic reconstruction may be performed with different reconstruction algorithms that can be grouped into several large families: direct Fourier inversion methods, back-projection methods, Radon methods, or iterative algorithms. In this review, we focus on the latter family of algorithms, explaining the mathematical rationale behind the different algorithms in this family as they have been introduced in the field of Electron Microscopy. We cover their use in Single Particle Analysis (SPA) as well as in Electron Tomography (ET). |
format | Online Article Text |
id | pubmed-5623807 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-56238072018-01-08 A Survey of the Use of Iterative Reconstruction Algorithms in Electron Microscopy Sorzano, C. O. S. Vargas, J. Otón, J. de la Rosa-Trevín, J. M. Vilas, J. L. Kazemi, M. Melero, R. del Caño, L. Cuenca, J. Conesa, P. Gómez-Blanco, J. Marabini, R. Carazo, J. M. Biomed Res Int Review Article One of the key steps in Electron Microscopy is the tomographic reconstruction of a three-dimensional (3D) map of the specimen being studied from a set of two-dimensional (2D) projections acquired at the microscope. This tomographic reconstruction may be performed with different reconstruction algorithms that can be grouped into several large families: direct Fourier inversion methods, back-projection methods, Radon methods, or iterative algorithms. In this review, we focus on the latter family of algorithms, explaining the mathematical rationale behind the different algorithms in this family as they have been introduced in the field of Electron Microscopy. We cover their use in Single Particle Analysis (SPA) as well as in Electron Tomography (ET). Hindawi 2017 2017-09-17 /pmc/articles/PMC5623807/ /pubmed/29312997 http://dx.doi.org/10.1155/2017/6482567 Text en Copyright © 2017 C. O. S. Sorzano et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Review Article Sorzano, C. O. S. Vargas, J. Otón, J. de la Rosa-Trevín, J. M. Vilas, J. L. Kazemi, M. Melero, R. del Caño, L. Cuenca, J. Conesa, P. Gómez-Blanco, J. Marabini, R. Carazo, J. M. A Survey of the Use of Iterative Reconstruction Algorithms in Electron Microscopy |
title | A Survey of the Use of Iterative Reconstruction Algorithms in Electron Microscopy |
title_full | A Survey of the Use of Iterative Reconstruction Algorithms in Electron Microscopy |
title_fullStr | A Survey of the Use of Iterative Reconstruction Algorithms in Electron Microscopy |
title_full_unstemmed | A Survey of the Use of Iterative Reconstruction Algorithms in Electron Microscopy |
title_short | A Survey of the Use of Iterative Reconstruction Algorithms in Electron Microscopy |
title_sort | survey of the use of iterative reconstruction algorithms in electron microscopy |
topic | Review Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5623807/ https://www.ncbi.nlm.nih.gov/pubmed/29312997 http://dx.doi.org/10.1155/2017/6482567 |
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