Cargando…

Molecular Characterization of Acanthamoeba Isolates from Surface Resting Waters in Northwest Iran

BACKGROUND: Acanthamoeba is an opportunistic amphizoic protozoan found in different fresh water sources. The aim of this study was to identify and characterize Acanthamoeba isolates from surface resting waters, in Northwest Iran. METHODS: Samples were collected from twenty-two different areas, betwe...

Descripción completa

Detalles Bibliográficos
Autores principales: FALLAH, Esmaeil, JAFARPOUR, Zahra, MAHAMI-OSKOUEI, Mahmoud, HAGHIGHI, Ali, NIYYATI, Maryam, SPOTIN, Adel, KHEZRI, Aram
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tehran University of Medical Sciences 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5623915/
https://www.ncbi.nlm.nih.gov/pubmed/28979345
_version_ 1783268172589170688
author FALLAH, Esmaeil
JAFARPOUR, Zahra
MAHAMI-OSKOUEI, Mahmoud
HAGHIGHI, Ali
NIYYATI, Maryam
SPOTIN, Adel
KHEZRI, Aram
author_facet FALLAH, Esmaeil
JAFARPOUR, Zahra
MAHAMI-OSKOUEI, Mahmoud
HAGHIGHI, Ali
NIYYATI, Maryam
SPOTIN, Adel
KHEZRI, Aram
author_sort FALLAH, Esmaeil
collection PubMed
description BACKGROUND: Acanthamoeba is an opportunistic amphizoic protozoan found in different fresh water sources. The aim of this study was to identify and characterize Acanthamoeba isolates from surface resting waters, in Northwest Iran. METHODS: Samples were collected from twenty-two different areas, between May and Sep 2014. After filtration, samples were cultivated on non-nutrient agar. The extracted DNAs were amplified and sequenced using partial 18S rRNA in order to genotype and phylogenetic analyses. RESULTS: Thirty-four (68%) out of 50 collected samples were positive for free-living amoebae based on both culture and morphological characterizations but 28 samples were identified as Acanthamoeba spp. by PCR. Sequentially, one isolate was identified as A. lenticulata, (T5) (AN: KP940443, identity 99.7%–100%, and divergence 0.3%) whilst other sequenced isolates identified Acanthamoeba spp. (AN: KP940444-45) as very similar to A. rhysodes and A. royreba with identity 100% and divergence 0%. CONCLUSION: Surface resting waters in Northwest Iran, were potentially contaminated with pathogenic amphizoic protozoan. Further studies will be required to determine other Acanthamoeba species and genotypes in the region.
format Online
Article
Text
id pubmed-5623915
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Tehran University of Medical Sciences
record_format MEDLINE/PubMed
spelling pubmed-56239152017-10-04 Molecular Characterization of Acanthamoeba Isolates from Surface Resting Waters in Northwest Iran FALLAH, Esmaeil JAFARPOUR, Zahra MAHAMI-OSKOUEI, Mahmoud HAGHIGHI, Ali NIYYATI, Maryam SPOTIN, Adel KHEZRI, Aram Iran J Parasitol Original Article BACKGROUND: Acanthamoeba is an opportunistic amphizoic protozoan found in different fresh water sources. The aim of this study was to identify and characterize Acanthamoeba isolates from surface resting waters, in Northwest Iran. METHODS: Samples were collected from twenty-two different areas, between May and Sep 2014. After filtration, samples were cultivated on non-nutrient agar. The extracted DNAs were amplified and sequenced using partial 18S rRNA in order to genotype and phylogenetic analyses. RESULTS: Thirty-four (68%) out of 50 collected samples were positive for free-living amoebae based on both culture and morphological characterizations but 28 samples were identified as Acanthamoeba spp. by PCR. Sequentially, one isolate was identified as A. lenticulata, (T5) (AN: KP940443, identity 99.7%–100%, and divergence 0.3%) whilst other sequenced isolates identified Acanthamoeba spp. (AN: KP940444-45) as very similar to A. rhysodes and A. royreba with identity 100% and divergence 0%. CONCLUSION: Surface resting waters in Northwest Iran, were potentially contaminated with pathogenic amphizoic protozoan. Further studies will be required to determine other Acanthamoeba species and genotypes in the region. Tehran University of Medical Sciences 2017 /pmc/articles/PMC5623915/ /pubmed/28979345 Text en Copyright© Iranian Society of Parasitology & Tehran University of Medical Sciences http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
FALLAH, Esmaeil
JAFARPOUR, Zahra
MAHAMI-OSKOUEI, Mahmoud
HAGHIGHI, Ali
NIYYATI, Maryam
SPOTIN, Adel
KHEZRI, Aram
Molecular Characterization of Acanthamoeba Isolates from Surface Resting Waters in Northwest Iran
title Molecular Characterization of Acanthamoeba Isolates from Surface Resting Waters in Northwest Iran
title_full Molecular Characterization of Acanthamoeba Isolates from Surface Resting Waters in Northwest Iran
title_fullStr Molecular Characterization of Acanthamoeba Isolates from Surface Resting Waters in Northwest Iran
title_full_unstemmed Molecular Characterization of Acanthamoeba Isolates from Surface Resting Waters in Northwest Iran
title_short Molecular Characterization of Acanthamoeba Isolates from Surface Resting Waters in Northwest Iran
title_sort molecular characterization of acanthamoeba isolates from surface resting waters in northwest iran
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5623915/
https://www.ncbi.nlm.nih.gov/pubmed/28979345
work_keys_str_mv AT fallahesmaeil molecularcharacterizationofacanthamoebaisolatesfromsurfacerestingwatersinnorthwestiran
AT jafarpourzahra molecularcharacterizationofacanthamoebaisolatesfromsurfacerestingwatersinnorthwestiran
AT mahamioskoueimahmoud molecularcharacterizationofacanthamoebaisolatesfromsurfacerestingwatersinnorthwestiran
AT haghighiali molecularcharacterizationofacanthamoebaisolatesfromsurfacerestingwatersinnorthwestiran
AT niyyatimaryam molecularcharacterizationofacanthamoebaisolatesfromsurfacerestingwatersinnorthwestiran
AT spotinadel molecularcharacterizationofacanthamoebaisolatesfromsurfacerestingwatersinnorthwestiran
AT khezriaram molecularcharacterizationofacanthamoebaisolatesfromsurfacerestingwatersinnorthwestiran