Cargando…
Molecular Characterization of Acanthamoeba Isolates from Surface Resting Waters in Northwest Iran
BACKGROUND: Acanthamoeba is an opportunistic amphizoic protozoan found in different fresh water sources. The aim of this study was to identify and characterize Acanthamoeba isolates from surface resting waters, in Northwest Iran. METHODS: Samples were collected from twenty-two different areas, betwe...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Tehran University of Medical Sciences
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5623915/ https://www.ncbi.nlm.nih.gov/pubmed/28979345 |
_version_ | 1783268172589170688 |
---|---|
author | FALLAH, Esmaeil JAFARPOUR, Zahra MAHAMI-OSKOUEI, Mahmoud HAGHIGHI, Ali NIYYATI, Maryam SPOTIN, Adel KHEZRI, Aram |
author_facet | FALLAH, Esmaeil JAFARPOUR, Zahra MAHAMI-OSKOUEI, Mahmoud HAGHIGHI, Ali NIYYATI, Maryam SPOTIN, Adel KHEZRI, Aram |
author_sort | FALLAH, Esmaeil |
collection | PubMed |
description | BACKGROUND: Acanthamoeba is an opportunistic amphizoic protozoan found in different fresh water sources. The aim of this study was to identify and characterize Acanthamoeba isolates from surface resting waters, in Northwest Iran. METHODS: Samples were collected from twenty-two different areas, between May and Sep 2014. After filtration, samples were cultivated on non-nutrient agar. The extracted DNAs were amplified and sequenced using partial 18S rRNA in order to genotype and phylogenetic analyses. RESULTS: Thirty-four (68%) out of 50 collected samples were positive for free-living amoebae based on both culture and morphological characterizations but 28 samples were identified as Acanthamoeba spp. by PCR. Sequentially, one isolate was identified as A. lenticulata, (T5) (AN: KP940443, identity 99.7%–100%, and divergence 0.3%) whilst other sequenced isolates identified Acanthamoeba spp. (AN: KP940444-45) as very similar to A. rhysodes and A. royreba with identity 100% and divergence 0%. CONCLUSION: Surface resting waters in Northwest Iran, were potentially contaminated with pathogenic amphizoic protozoan. Further studies will be required to determine other Acanthamoeba species and genotypes in the region. |
format | Online Article Text |
id | pubmed-5623915 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Tehran University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-56239152017-10-04 Molecular Characterization of Acanthamoeba Isolates from Surface Resting Waters in Northwest Iran FALLAH, Esmaeil JAFARPOUR, Zahra MAHAMI-OSKOUEI, Mahmoud HAGHIGHI, Ali NIYYATI, Maryam SPOTIN, Adel KHEZRI, Aram Iran J Parasitol Original Article BACKGROUND: Acanthamoeba is an opportunistic amphizoic protozoan found in different fresh water sources. The aim of this study was to identify and characterize Acanthamoeba isolates from surface resting waters, in Northwest Iran. METHODS: Samples were collected from twenty-two different areas, between May and Sep 2014. After filtration, samples were cultivated on non-nutrient agar. The extracted DNAs were amplified and sequenced using partial 18S rRNA in order to genotype and phylogenetic analyses. RESULTS: Thirty-four (68%) out of 50 collected samples were positive for free-living amoebae based on both culture and morphological characterizations but 28 samples were identified as Acanthamoeba spp. by PCR. Sequentially, one isolate was identified as A. lenticulata, (T5) (AN: KP940443, identity 99.7%–100%, and divergence 0.3%) whilst other sequenced isolates identified Acanthamoeba spp. (AN: KP940444-45) as very similar to A. rhysodes and A. royreba with identity 100% and divergence 0%. CONCLUSION: Surface resting waters in Northwest Iran, were potentially contaminated with pathogenic amphizoic protozoan. Further studies will be required to determine other Acanthamoeba species and genotypes in the region. Tehran University of Medical Sciences 2017 /pmc/articles/PMC5623915/ /pubmed/28979345 Text en Copyright© Iranian Society of Parasitology & Tehran University of Medical Sciences http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article FALLAH, Esmaeil JAFARPOUR, Zahra MAHAMI-OSKOUEI, Mahmoud HAGHIGHI, Ali NIYYATI, Maryam SPOTIN, Adel KHEZRI, Aram Molecular Characterization of Acanthamoeba Isolates from Surface Resting Waters in Northwest Iran |
title | Molecular Characterization of Acanthamoeba Isolates from Surface Resting Waters in Northwest Iran |
title_full | Molecular Characterization of Acanthamoeba Isolates from Surface Resting Waters in Northwest Iran |
title_fullStr | Molecular Characterization of Acanthamoeba Isolates from Surface Resting Waters in Northwest Iran |
title_full_unstemmed | Molecular Characterization of Acanthamoeba Isolates from Surface Resting Waters in Northwest Iran |
title_short | Molecular Characterization of Acanthamoeba Isolates from Surface Resting Waters in Northwest Iran |
title_sort | molecular characterization of acanthamoeba isolates from surface resting waters in northwest iran |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5623915/ https://www.ncbi.nlm.nih.gov/pubmed/28979345 |
work_keys_str_mv | AT fallahesmaeil molecularcharacterizationofacanthamoebaisolatesfromsurfacerestingwatersinnorthwestiran AT jafarpourzahra molecularcharacterizationofacanthamoebaisolatesfromsurfacerestingwatersinnorthwestiran AT mahamioskoueimahmoud molecularcharacterizationofacanthamoebaisolatesfromsurfacerestingwatersinnorthwestiran AT haghighiali molecularcharacterizationofacanthamoebaisolatesfromsurfacerestingwatersinnorthwestiran AT niyyatimaryam molecularcharacterizationofacanthamoebaisolatesfromsurfacerestingwatersinnorthwestiran AT spotinadel molecularcharacterizationofacanthamoebaisolatesfromsurfacerestingwatersinnorthwestiran AT khezriaram molecularcharacterizationofacanthamoebaisolatesfromsurfacerestingwatersinnorthwestiran |