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Strigolactones Improve Plant Growth, Photosynthesis, and Alleviate Oxidative Stress under Salinity in Rapeseed (Brassica napus L.) by Regulating Gene Expression
Rapeseed (Brassica napus L.) is a very important edible oil crop in the world, and the production is inhibited by abiotic stresses, such as salinity. Plant hormones can alleviate the stress by regulating the physiological processes and gene expression. To study the plant responses to salinity in com...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5623956/ https://www.ncbi.nlm.nih.gov/pubmed/29021800 http://dx.doi.org/10.3389/fpls.2017.01671 |
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author | Ma, Ni Hu, Chao Wan, Lin Hu, Qiong Xiong, Junlan Zhang, Chunlei |
author_facet | Ma, Ni Hu, Chao Wan, Lin Hu, Qiong Xiong, Junlan Zhang, Chunlei |
author_sort | Ma, Ni |
collection | PubMed |
description | Rapeseed (Brassica napus L.) is a very important edible oil crop in the world, and the production is inhibited by abiotic stresses, such as salinity. Plant hormones can alleviate the stress by regulating the physiological processes and gene expression. To study the plant responses to salinity in combination with GR24, a synthesized strigolactone, the oilseed rape variety (Zhongshuang 11) replications were grown in the pots in a controlled growth chamber under three levels of salinity (0, 100, and 200 mM NaCl) and 0.18 μM GR24 treatments at the seedling stage for 7 days. The results showed that salinity depressed the shoots and roots growth, whereas GR24 improved the growth under salt stress. Leaf chlorophyll contents and gas exchange parameters (net photosynthetic rates, stomatal conductance, intercellular CO(2) concentration, and transpiration rate) were also reduced significantly with increasing salinity, and these effects could be partially reversed by GR24 application. Additionally, GR24 treatment significantly increased and decreased the photosystem II quantum yield and non-photochemical quenching, respectively, under salinity stress conditions. The activities of peroxidase and superoxide dismutase increased, and lipid peroxidation measured by the level of malondialdehyde reduced due to GR24 application. The transcriptome analysis of root and shoot was conducted. Three hundred and forty-two common differentially expressed genes (DEGs) after GR24 treatment and 166 special DEGs after GR24 treatment under salinity stress were identified in root and shoot. The DEGs in root were significantly more than that in shoot. Quantitative PCR validated that the stress alleviation was mainly related to the gene expression of tryptophan metabolism, plant hormone signal transduction, and photosynthesis. |
format | Online Article Text |
id | pubmed-5623956 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-56239562017-10-11 Strigolactones Improve Plant Growth, Photosynthesis, and Alleviate Oxidative Stress under Salinity in Rapeseed (Brassica napus L.) by Regulating Gene Expression Ma, Ni Hu, Chao Wan, Lin Hu, Qiong Xiong, Junlan Zhang, Chunlei Front Plant Sci Plant Science Rapeseed (Brassica napus L.) is a very important edible oil crop in the world, and the production is inhibited by abiotic stresses, such as salinity. Plant hormones can alleviate the stress by regulating the physiological processes and gene expression. To study the plant responses to salinity in combination with GR24, a synthesized strigolactone, the oilseed rape variety (Zhongshuang 11) replications were grown in the pots in a controlled growth chamber under three levels of salinity (0, 100, and 200 mM NaCl) and 0.18 μM GR24 treatments at the seedling stage for 7 days. The results showed that salinity depressed the shoots and roots growth, whereas GR24 improved the growth under salt stress. Leaf chlorophyll contents and gas exchange parameters (net photosynthetic rates, stomatal conductance, intercellular CO(2) concentration, and transpiration rate) were also reduced significantly with increasing salinity, and these effects could be partially reversed by GR24 application. Additionally, GR24 treatment significantly increased and decreased the photosystem II quantum yield and non-photochemical quenching, respectively, under salinity stress conditions. The activities of peroxidase and superoxide dismutase increased, and lipid peroxidation measured by the level of malondialdehyde reduced due to GR24 application. The transcriptome analysis of root and shoot was conducted. Three hundred and forty-two common differentially expressed genes (DEGs) after GR24 treatment and 166 special DEGs after GR24 treatment under salinity stress were identified in root and shoot. The DEGs in root were significantly more than that in shoot. Quantitative PCR validated that the stress alleviation was mainly related to the gene expression of tryptophan metabolism, plant hormone signal transduction, and photosynthesis. Frontiers Media S.A. 2017-09-27 /pmc/articles/PMC5623956/ /pubmed/29021800 http://dx.doi.org/10.3389/fpls.2017.01671 Text en Copyright © 2017 Ma, Hu, Wan, Hu, Xiong and Zhang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Ma, Ni Hu, Chao Wan, Lin Hu, Qiong Xiong, Junlan Zhang, Chunlei Strigolactones Improve Plant Growth, Photosynthesis, and Alleviate Oxidative Stress under Salinity in Rapeseed (Brassica napus L.) by Regulating Gene Expression |
title | Strigolactones Improve Plant Growth, Photosynthesis, and Alleviate Oxidative Stress under Salinity in Rapeseed (Brassica napus L.) by Regulating Gene Expression |
title_full | Strigolactones Improve Plant Growth, Photosynthesis, and Alleviate Oxidative Stress under Salinity in Rapeseed (Brassica napus L.) by Regulating Gene Expression |
title_fullStr | Strigolactones Improve Plant Growth, Photosynthesis, and Alleviate Oxidative Stress under Salinity in Rapeseed (Brassica napus L.) by Regulating Gene Expression |
title_full_unstemmed | Strigolactones Improve Plant Growth, Photosynthesis, and Alleviate Oxidative Stress under Salinity in Rapeseed (Brassica napus L.) by Regulating Gene Expression |
title_short | Strigolactones Improve Plant Growth, Photosynthesis, and Alleviate Oxidative Stress under Salinity in Rapeseed (Brassica napus L.) by Regulating Gene Expression |
title_sort | strigolactones improve plant growth, photosynthesis, and alleviate oxidative stress under salinity in rapeseed (brassica napus l.) by regulating gene expression |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5623956/ https://www.ncbi.nlm.nih.gov/pubmed/29021800 http://dx.doi.org/10.3389/fpls.2017.01671 |
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