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Application of HPTLC with Densitometry for Evaluation of the Impact of External Factors on Contents of Diphenhydramine in Its Solutions
The subject of durability of drugs is a very important problem investigated by researches. The method of accelerated aging is very often used for stability testing. It involves the influence of temperature, humidity, and light exposure. The aim of this work is estimation of contents of diphenhydrami...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Hindawi
2017
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5624158/ https://www.ncbi.nlm.nih.gov/pubmed/29075295 http://dx.doi.org/10.1155/2017/4914292 |
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author | Bober, Katarzyna |
author_facet | Bober, Katarzyna |
author_sort | Bober, Katarzyna |
collection | PubMed |
description | The subject of durability of drugs is a very important problem investigated by researches. The method of accelerated aging is very often used for stability testing. It involves the influence of temperature, humidity, and light exposure. The aim of this work is estimation of contents of diphenhydramine in its standard solutions undergoing the impact of external factors, that is, temperature and UV light. The standard solutions of diphenhydramine were prepared in distilled water. The analysis of contents of compound investigated was carried out by use of HPTLC. Adsorption thin layer chromatography was performed on aluminum HPTLC plates precoated with silica gel 60F(254) using mixture of ammonia, methanol, and ethyl acetate as mobile phase. The solutions investigated were exposed to UV light as well as being heated in 40°C. The contents of diphenhydramine were measured in initial solution and during experiment. The additional peaks on densitograms as well as changes in color of solution were observed as a result of exposure to UV light which can give information about new substances, degradation products of diphenhydramine, which were created during experiment. |
format | Online Article Text |
id | pubmed-5624158 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-56241582017-10-26 Application of HPTLC with Densitometry for Evaluation of the Impact of External Factors on Contents of Diphenhydramine in Its Solutions Bober, Katarzyna Int J Anal Chem Research Article The subject of durability of drugs is a very important problem investigated by researches. The method of accelerated aging is very often used for stability testing. It involves the influence of temperature, humidity, and light exposure. The aim of this work is estimation of contents of diphenhydramine in its standard solutions undergoing the impact of external factors, that is, temperature and UV light. The standard solutions of diphenhydramine were prepared in distilled water. The analysis of contents of compound investigated was carried out by use of HPTLC. Adsorption thin layer chromatography was performed on aluminum HPTLC plates precoated with silica gel 60F(254) using mixture of ammonia, methanol, and ethyl acetate as mobile phase. The solutions investigated were exposed to UV light as well as being heated in 40°C. The contents of diphenhydramine were measured in initial solution and during experiment. The additional peaks on densitograms as well as changes in color of solution were observed as a result of exposure to UV light which can give information about new substances, degradation products of diphenhydramine, which were created during experiment. Hindawi 2017 2017-09-18 /pmc/articles/PMC5624158/ /pubmed/29075295 http://dx.doi.org/10.1155/2017/4914292 Text en Copyright © 2017 Katarzyna Bober. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Bober, Katarzyna Application of HPTLC with Densitometry for Evaluation of the Impact of External Factors on Contents of Diphenhydramine in Its Solutions |
title | Application of HPTLC with Densitometry for Evaluation of the Impact of External Factors on Contents of Diphenhydramine in Its Solutions |
title_full | Application of HPTLC with Densitometry for Evaluation of the Impact of External Factors on Contents of Diphenhydramine in Its Solutions |
title_fullStr | Application of HPTLC with Densitometry for Evaluation of the Impact of External Factors on Contents of Diphenhydramine in Its Solutions |
title_full_unstemmed | Application of HPTLC with Densitometry for Evaluation of the Impact of External Factors on Contents of Diphenhydramine in Its Solutions |
title_short | Application of HPTLC with Densitometry for Evaluation of the Impact of External Factors on Contents of Diphenhydramine in Its Solutions |
title_sort | application of hptlc with densitometry for evaluation of the impact of external factors on contents of diphenhydramine in its solutions |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5624158/ https://www.ncbi.nlm.nih.gov/pubmed/29075295 http://dx.doi.org/10.1155/2017/4914292 |
work_keys_str_mv | AT boberkatarzyna applicationofhptlcwithdensitometryforevaluationoftheimpactofexternalfactorsoncontentsofdiphenhydramineinitssolutions |