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Identifying and sequencing a Mycobacterium sp. strain F4 as a potential bioremediation agent for quinclorac

Quinclorac is a widely used herbicide in rice filed. Unfortunately, quinclorac residues are phytotoxic to many crops/vegetables. The degradation of quinclorac in nature is very slow. On the other hand, degradation of quinclorac using bacteria can be an effective and efficient method to reduce its co...

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Autores principales: Li, Yingying, Chen, Wu, Wang, Yunsheng, Luo, Kun, Li, Yue, Bai, Lianyang, Luo, Feng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5624592/
https://www.ncbi.nlm.nih.gov/pubmed/28968436
http://dx.doi.org/10.1371/journal.pone.0185721
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author Li, Yingying
Chen, Wu
Wang, Yunsheng
Luo, Kun
Li, Yue
Bai, Lianyang
Luo, Feng
author_facet Li, Yingying
Chen, Wu
Wang, Yunsheng
Luo, Kun
Li, Yue
Bai, Lianyang
Luo, Feng
author_sort Li, Yingying
collection PubMed
description Quinclorac is a widely used herbicide in rice filed. Unfortunately, quinclorac residues are phytotoxic to many crops/vegetables. The degradation of quinclorac in nature is very slow. On the other hand, degradation of quinclorac using bacteria can be an effective and efficient method to reduce its contamination. In this study, we isolated a quinclorac bioremediation bacterium strain F4 from quinclorac contaminated soils. Based on morphological characteristics and 16S rRNA gene sequence analysis, we identified strain F4 as Mycobacterium sp. We investigated the effects of temperature, pH, inoculation size and initial quinclorac concentration on growth and degrading efficiency of F4 and determined the optimal quinclorac degrading condition of F4. Under optimal degrading conditions, F4 degraded 97.38% of quinclorac from an initial concentration of 50 mg/L in seven days. Our indoor pot experiment demonstrated that the degradation products were non-phytotoxic to tobacco. After analyzing the quinclorac degradation products of F4, we proposed that F4 could employ two pathways to degrade quinclorac: one is through methylation, the other is through dechlorination. Furthermore, we reconstructed the whole genome of F4 through single molecular sequencing and de novo assembly. We identified 77 methyltransferases and eight dehalogenases in the F4 genome to support our hypothesized degradation path.
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spelling pubmed-56245922017-10-17 Identifying and sequencing a Mycobacterium sp. strain F4 as a potential bioremediation agent for quinclorac Li, Yingying Chen, Wu Wang, Yunsheng Luo, Kun Li, Yue Bai, Lianyang Luo, Feng PLoS One Research Article Quinclorac is a widely used herbicide in rice filed. Unfortunately, quinclorac residues are phytotoxic to many crops/vegetables. The degradation of quinclorac in nature is very slow. On the other hand, degradation of quinclorac using bacteria can be an effective and efficient method to reduce its contamination. In this study, we isolated a quinclorac bioremediation bacterium strain F4 from quinclorac contaminated soils. Based on morphological characteristics and 16S rRNA gene sequence analysis, we identified strain F4 as Mycobacterium sp. We investigated the effects of temperature, pH, inoculation size and initial quinclorac concentration on growth and degrading efficiency of F4 and determined the optimal quinclorac degrading condition of F4. Under optimal degrading conditions, F4 degraded 97.38% of quinclorac from an initial concentration of 50 mg/L in seven days. Our indoor pot experiment demonstrated that the degradation products were non-phytotoxic to tobacco. After analyzing the quinclorac degradation products of F4, we proposed that F4 could employ two pathways to degrade quinclorac: one is through methylation, the other is through dechlorination. Furthermore, we reconstructed the whole genome of F4 through single molecular sequencing and de novo assembly. We identified 77 methyltransferases and eight dehalogenases in the F4 genome to support our hypothesized degradation path. Public Library of Science 2017-10-02 /pmc/articles/PMC5624592/ /pubmed/28968436 http://dx.doi.org/10.1371/journal.pone.0185721 Text en © 2017 Li et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Li, Yingying
Chen, Wu
Wang, Yunsheng
Luo, Kun
Li, Yue
Bai, Lianyang
Luo, Feng
Identifying and sequencing a Mycobacterium sp. strain F4 as a potential bioremediation agent for quinclorac
title Identifying and sequencing a Mycobacterium sp. strain F4 as a potential bioremediation agent for quinclorac
title_full Identifying and sequencing a Mycobacterium sp. strain F4 as a potential bioremediation agent for quinclorac
title_fullStr Identifying and sequencing a Mycobacterium sp. strain F4 as a potential bioremediation agent for quinclorac
title_full_unstemmed Identifying and sequencing a Mycobacterium sp. strain F4 as a potential bioremediation agent for quinclorac
title_short Identifying and sequencing a Mycobacterium sp. strain F4 as a potential bioremediation agent for quinclorac
title_sort identifying and sequencing a mycobacterium sp. strain f4 as a potential bioremediation agent for quinclorac
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5624592/
https://www.ncbi.nlm.nih.gov/pubmed/28968436
http://dx.doi.org/10.1371/journal.pone.0185721
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