Complete Draft Genome Sequence of Escherichia coli KRX, a Strain for Efficient Cloning and High-Yield Expression of Proteins under Control of the T7 RNA Polymerase

Escherichia coli KRX is a strain offering both a high transformation efficiency and the possibility to produce the target protein to high yields in one host, avoiding additional cloning steps. Here, the draft genome sequence of E. coli KRX is presented and provides the genetic basis for additional b...

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Autores principales: Schwarzhans, Jan-Philipp, Wibberg, Daniel, Winkler, Anika, Kalinowski, Jörn, Friehs, Karl
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2017
Materias:
Prokaryotes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5624749/
https://www.ncbi.nlm.nih.gov/pubmed/28963203
http://dx.doi.org/10.1128/genomeA.00933-17
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author Schwarzhans, Jan-Philipp
Wibberg, Daniel
Winkler, Anika
Kalinowski, Jörn
Friehs, Karl
author_facet Schwarzhans, Jan-Philipp
Wibberg, Daniel
Winkler, Anika
Kalinowski, Jörn
Friehs, Karl
author_sort Schwarzhans, Jan-Philipp
collection PubMed
description Escherichia coli KRX is a strain offering both a high transformation efficiency and the possibility to produce the target protein to high yields in one host, avoiding additional cloning steps. Here, the draft genome sequence of E. coli KRX is presented and provides the genetic basis for additional biotechnological applications.
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spelling pubmed-56247492017-10-04 Complete Draft Genome Sequence of Escherichia coli KRX, a Strain for Efficient Cloning and High-Yield Expression of Proteins under Control of the T7 RNA Polymerase Schwarzhans, Jan-Philipp Wibberg, Daniel Winkler, Anika Kalinowski, Jörn Friehs, Karl Genome Announc Prokaryotes Escherichia coli KRX is a strain offering both a high transformation efficiency and the possibility to produce the target protein to high yields in one host, avoiding additional cloning steps. Here, the draft genome sequence of E. coli KRX is presented and provides the genetic basis for additional biotechnological applications. American Society for Microbiology 2017-09-28 /pmc/articles/PMC5624749/ /pubmed/28963203 http://dx.doi.org/10.1128/genomeA.00933-17 Text en Copyright © 2017 Schwarzhans et al. https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Prokaryotes
Schwarzhans, Jan-Philipp
Wibberg, Daniel
Winkler, Anika
Kalinowski, Jörn
Friehs, Karl
Complete Draft Genome Sequence of Escherichia coli KRX, a Strain for Efficient Cloning and High-Yield Expression of Proteins under Control of the T7 RNA Polymerase
title Complete Draft Genome Sequence of Escherichia coli KRX, a Strain for Efficient Cloning and High-Yield Expression of Proteins under Control of the T7 RNA Polymerase
title_full Complete Draft Genome Sequence of Escherichia coli KRX, a Strain for Efficient Cloning and High-Yield Expression of Proteins under Control of the T7 RNA Polymerase
title_fullStr Complete Draft Genome Sequence of Escherichia coli KRX, a Strain for Efficient Cloning and High-Yield Expression of Proteins under Control of the T7 RNA Polymerase
title_full_unstemmed Complete Draft Genome Sequence of Escherichia coli KRX, a Strain for Efficient Cloning and High-Yield Expression of Proteins under Control of the T7 RNA Polymerase
title_short Complete Draft Genome Sequence of Escherichia coli KRX, a Strain for Efficient Cloning and High-Yield Expression of Proteins under Control of the T7 RNA Polymerase
title_sort complete draft genome sequence of escherichia coli krx, a strain for efficient cloning and high-yield expression of proteins under control of the t7 rna polymerase
topic Prokaryotes
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5624749/
https://www.ncbi.nlm.nih.gov/pubmed/28963203
http://dx.doi.org/10.1128/genomeA.00933-17
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