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Intraretinal Correlates of Reticular Pseudodrusen Revealed by Autofluorescence and En Face OCT

PURPOSE: We sought to determine whether information revealed from the reflectance, autofluorescence, and absorption properties of RPE cells situated posterior to reticular pseudodrusen (RPD) could provide insight into the origins and structure of RPD. METHODS: RPD were studied qualitatively by near-...

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Autores principales: Paavo, Maarjaliis, Lee, Winston, Merriam, John, Bearelly, Srilaxmi, Tsang, Stephen, Chang, Stanley, Sparrow, Janet R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Association for Research in Vision and Ophthalmology 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5624777/
https://www.ncbi.nlm.nih.gov/pubmed/28973322
http://dx.doi.org/10.1167/iovs.17-22338
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author Paavo, Maarjaliis
Lee, Winston
Merriam, John
Bearelly, Srilaxmi
Tsang, Stephen
Chang, Stanley
Sparrow, Janet R.
author_facet Paavo, Maarjaliis
Lee, Winston
Merriam, John
Bearelly, Srilaxmi
Tsang, Stephen
Chang, Stanley
Sparrow, Janet R.
author_sort Paavo, Maarjaliis
collection PubMed
description PURPOSE: We sought to determine whether information revealed from the reflectance, autofluorescence, and absorption properties of RPE cells situated posterior to reticular pseudodrusen (RPD) could provide insight into the origins and structure of RPD. METHODS: RPD were studied qualitatively by near-infrared fundus autofluorescence (NIR-AF), short-wavelength fundus autofluorescence (SW-AF), and infrared reflectance (IR-R) images, and the presentation was compared to horizontal and en face spectral domain optical coherence tomographic (SD-OCT) images. Images were acquired from 23 patients (39 eyes) diagnosed with RPD (mean age 80.7 ± 7.1 [SD]; 16 female; 4 Hispanics, 19 non-Hispanic whites). RESULTS: In SW-AF, NIR-AF, and IR-R images, fundus RPD were recognized as interlacing networks of small scale variations in IR-R and fluorescence (SW-AF, NIR-AF) intensities. Darkened foci of RPD colocalized in SW-AF and NIR-AF images, and in SD-OCT images corresponded to disturbances of the interdigitation (IZ) and ellipsoid (EZ) zones and to more pronounced hyperreflective lesions traversing photoreceptor-attributable bands in SD-OCT images. Qualitative assessment of the outer nuclear layer (ONL) revealed thinning as RPD extended radially from the outer to inner retina. In en face OCT, hyperreflective areas in the EZ band correlated topographically with hyporeflective foci at the level of the RPE. CONCLUSIONS: The hyperreflective lesions corresponding to RPD in SD-OCT scans are likely indicative of degenerating photoreceptor cells. The darkened foci at positions of RPD in NIR-AF and en face OCT images indicate changes in the RPE monolayer with the reduced NIR-AF and en face OCT signal suggesting a reduction in melanin that could be accounted for by RPE thinning.
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spelling pubmed-56247772017-10-03 Intraretinal Correlates of Reticular Pseudodrusen Revealed by Autofluorescence and En Face OCT Paavo, Maarjaliis Lee, Winston Merriam, John Bearelly, Srilaxmi Tsang, Stephen Chang, Stanley Sparrow, Janet R. Invest Ophthalmol Vis Sci Retina PURPOSE: We sought to determine whether information revealed from the reflectance, autofluorescence, and absorption properties of RPE cells situated posterior to reticular pseudodrusen (RPD) could provide insight into the origins and structure of RPD. METHODS: RPD were studied qualitatively by near-infrared fundus autofluorescence (NIR-AF), short-wavelength fundus autofluorescence (SW-AF), and infrared reflectance (IR-R) images, and the presentation was compared to horizontal and en face spectral domain optical coherence tomographic (SD-OCT) images. Images were acquired from 23 patients (39 eyes) diagnosed with RPD (mean age 80.7 ± 7.1 [SD]; 16 female; 4 Hispanics, 19 non-Hispanic whites). RESULTS: In SW-AF, NIR-AF, and IR-R images, fundus RPD were recognized as interlacing networks of small scale variations in IR-R and fluorescence (SW-AF, NIR-AF) intensities. Darkened foci of RPD colocalized in SW-AF and NIR-AF images, and in SD-OCT images corresponded to disturbances of the interdigitation (IZ) and ellipsoid (EZ) zones and to more pronounced hyperreflective lesions traversing photoreceptor-attributable bands in SD-OCT images. Qualitative assessment of the outer nuclear layer (ONL) revealed thinning as RPD extended radially from the outer to inner retina. In en face OCT, hyperreflective areas in the EZ band correlated topographically with hyporeflective foci at the level of the RPE. CONCLUSIONS: The hyperreflective lesions corresponding to RPD in SD-OCT scans are likely indicative of degenerating photoreceptor cells. The darkened foci at positions of RPD in NIR-AF and en face OCT images indicate changes in the RPE monolayer with the reduced NIR-AF and en face OCT signal suggesting a reduction in melanin that could be accounted for by RPE thinning. The Association for Research in Vision and Ophthalmology 2017-10 /pmc/articles/PMC5624777/ /pubmed/28973322 http://dx.doi.org/10.1167/iovs.17-22338 Text en Copyright 2017 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
spellingShingle Retina
Paavo, Maarjaliis
Lee, Winston
Merriam, John
Bearelly, Srilaxmi
Tsang, Stephen
Chang, Stanley
Sparrow, Janet R.
Intraretinal Correlates of Reticular Pseudodrusen Revealed by Autofluorescence and En Face OCT
title Intraretinal Correlates of Reticular Pseudodrusen Revealed by Autofluorescence and En Face OCT
title_full Intraretinal Correlates of Reticular Pseudodrusen Revealed by Autofluorescence and En Face OCT
title_fullStr Intraretinal Correlates of Reticular Pseudodrusen Revealed by Autofluorescence and En Face OCT
title_full_unstemmed Intraretinal Correlates of Reticular Pseudodrusen Revealed by Autofluorescence and En Face OCT
title_short Intraretinal Correlates of Reticular Pseudodrusen Revealed by Autofluorescence and En Face OCT
title_sort intraretinal correlates of reticular pseudodrusen revealed by autofluorescence and en face oct
topic Retina
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5624777/
https://www.ncbi.nlm.nih.gov/pubmed/28973322
http://dx.doi.org/10.1167/iovs.17-22338
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