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Acidic and enzymatic saccharification of waste agricultural biomass for biotechnological production of xylitol

BACKGROUND: The plant biomass and agro-industrial wastes show great potential for their use as attractive low cost substrates in biotechnological processes. Wheat straw and corn cob as hemicellulosic substrates were acid hydrolyzed and enzymatically saccharified for high xylose production. The hydro...

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Autores principales: Ghaffar, Abdul, Yameen, Muhammad, Aslam, Nosheen, Jalal, Fatima, Noreen, Razia, Munir, Bushra, Mahmood, Zahed, Saleem, Sadaf, Rafiq, Naila, Falak, Sadia, Tahir, Imtiaz Mahmood, Noman, Muhammad, Farooq, Muhammad Umar, Qasim, Samina, Latif, Farooq
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5624859/
https://www.ncbi.nlm.nih.gov/pubmed/29086883
http://dx.doi.org/10.1186/s13065-017-0331-z
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author Ghaffar, Abdul
Yameen, Muhammad
Aslam, Nosheen
Jalal, Fatima
Noreen, Razia
Munir, Bushra
Mahmood, Zahed
Saleem, Sadaf
Rafiq, Naila
Falak, Sadia
Tahir, Imtiaz Mahmood
Noman, Muhammad
Farooq, Muhammad Umar
Qasim, Samina
Latif, Farooq
author_facet Ghaffar, Abdul
Yameen, Muhammad
Aslam, Nosheen
Jalal, Fatima
Noreen, Razia
Munir, Bushra
Mahmood, Zahed
Saleem, Sadaf
Rafiq, Naila
Falak, Sadia
Tahir, Imtiaz Mahmood
Noman, Muhammad
Farooq, Muhammad Umar
Qasim, Samina
Latif, Farooq
author_sort Ghaffar, Abdul
collection PubMed
description BACKGROUND: The plant biomass and agro-industrial wastes show great potential for their use as attractive low cost substrates in biotechnological processes. Wheat straw and corn cob as hemicellulosic substrates were acid hydrolyzed and enzymatically saccharified for high xylose production. The hydrolysate was concentrated and fermented by using Saccharomyces cerevisiae and Kluyveromyces for production of xylitol. RESULTS: Acid hydrolysis of wheat straw and corn cob in combination with enzymatic hydrolysis showed great potential for production of free sugars from these substrates. Kluyveromyces produced maximum xylitol from acid treated wheat straw residues with enzymatic saccharification. The percentage xylitol yield was 89.807 g/L and volumetric productivity of 0.019 g/L/h. Kluyveromyces also produced maximum xylitol from corn cob acid hydrolyzed liquor with xylitol yield 87.716 g/L and volumetric productivity 0.018 g/L/h. CONCLUSION: Plant and agro-industrial biomass can be used as a carbohydrate source for the production of xylitol and ethanol after microbial fermentation. This study revealed that wheat straw acid and enzyme hydrolyzed residue proved to be best raw material for production of xylitol with S. cerevisiae. The xylitol produced can be utilized in pharmaceuticals after purification on industrial scale as pharmaceutical purposes.
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spelling pubmed-56248592017-10-17 Acidic and enzymatic saccharification of waste agricultural biomass for biotechnological production of xylitol Ghaffar, Abdul Yameen, Muhammad Aslam, Nosheen Jalal, Fatima Noreen, Razia Munir, Bushra Mahmood, Zahed Saleem, Sadaf Rafiq, Naila Falak, Sadia Tahir, Imtiaz Mahmood Noman, Muhammad Farooq, Muhammad Umar Qasim, Samina Latif, Farooq Chem Cent J Research Article BACKGROUND: The plant biomass and agro-industrial wastes show great potential for their use as attractive low cost substrates in biotechnological processes. Wheat straw and corn cob as hemicellulosic substrates were acid hydrolyzed and enzymatically saccharified for high xylose production. The hydrolysate was concentrated and fermented by using Saccharomyces cerevisiae and Kluyveromyces for production of xylitol. RESULTS: Acid hydrolysis of wheat straw and corn cob in combination with enzymatic hydrolysis showed great potential for production of free sugars from these substrates. Kluyveromyces produced maximum xylitol from acid treated wheat straw residues with enzymatic saccharification. The percentage xylitol yield was 89.807 g/L and volumetric productivity of 0.019 g/L/h. Kluyveromyces also produced maximum xylitol from corn cob acid hydrolyzed liquor with xylitol yield 87.716 g/L and volumetric productivity 0.018 g/L/h. CONCLUSION: Plant and agro-industrial biomass can be used as a carbohydrate source for the production of xylitol and ethanol after microbial fermentation. This study revealed that wheat straw acid and enzyme hydrolyzed residue proved to be best raw material for production of xylitol with S. cerevisiae. The xylitol produced can be utilized in pharmaceuticals after purification on industrial scale as pharmaceutical purposes. Springer International Publishing 2017-10-02 /pmc/articles/PMC5624859/ /pubmed/29086883 http://dx.doi.org/10.1186/s13065-017-0331-z Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Ghaffar, Abdul
Yameen, Muhammad
Aslam, Nosheen
Jalal, Fatima
Noreen, Razia
Munir, Bushra
Mahmood, Zahed
Saleem, Sadaf
Rafiq, Naila
Falak, Sadia
Tahir, Imtiaz Mahmood
Noman, Muhammad
Farooq, Muhammad Umar
Qasim, Samina
Latif, Farooq
Acidic and enzymatic saccharification of waste agricultural biomass for biotechnological production of xylitol
title Acidic and enzymatic saccharification of waste agricultural biomass for biotechnological production of xylitol
title_full Acidic and enzymatic saccharification of waste agricultural biomass for biotechnological production of xylitol
title_fullStr Acidic and enzymatic saccharification of waste agricultural biomass for biotechnological production of xylitol
title_full_unstemmed Acidic and enzymatic saccharification of waste agricultural biomass for biotechnological production of xylitol
title_short Acidic and enzymatic saccharification of waste agricultural biomass for biotechnological production of xylitol
title_sort acidic and enzymatic saccharification of waste agricultural biomass for biotechnological production of xylitol
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5624859/
https://www.ncbi.nlm.nih.gov/pubmed/29086883
http://dx.doi.org/10.1186/s13065-017-0331-z
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