Cargando…

Functional Analysis of Phagocyte Activity in Whole Blood from HIV/Tuberculosis-Infected Individuals Using a Novel Flow Cytometry-Based Assay

The accurate assessment of immune competence through ex vivo analysis is paramount to our understanding of those immune mechanisms that lead to protection or susceptibility against a broad range of human pathogens. We have developed a flow cytometry-based, whole blood phagocyte functional assay that...

Descripción completa

Detalles Bibliográficos
Autores principales:	Gupta-Wright, Ankur, Tembo, Dumizulu, Jambo, Kondwani C., Chimbayo, Elizabeth, Mvaya, Leonard, Caldwell, Shannon, Russell, David G., Mwandumba, Henry C.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2017
Materias:	Immunology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5624998/ https://www.ncbi.nlm.nih.gov/pubmed/29033941 http://dx.doi.org/10.3389/fimmu.2017.01222

Ejemplares similares

TZM-gfp cells: a tractable fluorescent tool for analysis of rare and early HIV-1 infection
por: Gludish, David W., et al.
Publicado: (2020)

B cell, CD8 (+) T cell and gamma delta T cell infiltration alters alveolar immune cell homeostasis in HIV-infected Malawian adults
por: Mwale, Andrew, et al.
Publicado: (2018)

Airway CD8(+)CD161(++)TCRvα7.2(+) T Cell Depletion During Untreated HIV Infection Targets CD103 Expressing Cells
por: Mvaya, Leonard, et al.
Publicado: (2019)

Something Old, Something New: Ion Channel Blockers as Potential Anti-Tuberculosis Agents
por: Mitini-Nkhoma, Steven C., et al.
Publicado: (2021)

Inhibition of the lncRNA SAF drives activation of apoptotic effector caspases in HIV-1–infected human macrophages
por: Boliar, Saikat, et al.
Publicado: (2019)

Latent tuberculosis infection among adults attending HIV services at an urban tertiary hospital in Malawi
por: Mitini-Nkhoma, Steven C., et al.
Publicado: (2022)

Loss of Humoral and Cellular Immunity to Invasive Nontyphoidal Salmonella during Current or Convalescent Plasmodium falciparum Infection in Malawian Children
por: Nyirenda, Tonney S., et al.
Publicado: (2017)

HIV-associated disruption of lung cytokine networks is incompletely restored in asymptomatic HIV-infected Malawian adults on antiretroviral therapy
por: Jambo, Kondwani C., et al.
Publicado: (2017)

Heterogeneous loss of HIV transcription and proviral DNA from 8E5/LAV lymphoblastic leukemia cells revealed by RNA FISH:FLOW analyses
por: Wilburn, Kaley M., et al.
Publicado: (2016)

Small alveolar macrophages are infected preferentially by HIV and exhibit impaired phagocytic function
por: Jambo, K C, et al.
Publicado: (2014)

A Simplified, Sensitive Phagocytic Assay for Malaria Cultures Facilitated by Flow Cytometry of Differentially-Stained Cell Populations
por: Chan, Chuu Ling, et al.
Publicado: (2012)

Defective Monocyte Enzymatic Function and an Inhibitory Immune Phenotype in Human Immunodeficiency Virus-Exposed Uninfected African Infants in the Era of Antiretroviral Therapy( )
por: Afran, Louise, et al.
Publicado: (2022)

A flow cytometry‐based assay to determine the phagocytic activity of both clinical and nonclinical antibody samples against Chlamydia trachomatis
por: Grasse, Marco, et al.
Publicado: (2018)

Differential localization and limited cytotoxic potential of duodenal CD8(+) T cells
por: Mvaya, Leonard, et al.
Publicado: (2022)

Standardization of cytokine flow cytometry assays
por: Maecker, Holden T, et al.
Publicado: (2005)

Using flow cytometry for mitochondrial assays
por: Monteiro, Lauar de Brito, et al.
Publicado: (2020)

Trypan blue exclusion assay by flow cytometry
por: Avelar-Freitas, B.A., et al.
Publicado: (2014)

Quantification of Bioorthogonal Stability in Immune Phagocytes Using Flow Cytometry Reveals Rapid Degradation of Strained Alkynes
por: Bakkum, Thomas, et al.
Publicado: (2018)

A flow cytometry approach reveals heterogeneity in conventional subsets of murine renal mononuclear phagocytes
por: Nordlohne, Johannes, et al.
Publicado: (2021)

Alveolar T-helper 17 responses to streptococcus pneumoniae are preserved in ART-untreated and treated HIV-infected Malawian adults
por: Peno, Chikondi, et al.
Publicado: (2018)

First case report of a successfully managed severe COVID-19 infection in Malawi
por: Banda, Ndaziona Peter, et al.
Publicado: (2020)

Human alveolar macrophages predominately express combined classical M1 and M2 surface markers in steady state
por: Mitsi, Elena, et al.
Publicado: (2018)

Immune cell dysfunctions in breast cancer patients detected through whole blood multi-parametric flow cytometry assay
por: Verronèse, E., et al.
Publicado: (2015)

Mapping Cell Phenomics with Multiparametric Flow Cytometry Assays
por: Liu, Yang, et al.
Publicado: (2022)

Development of a Modular Assay for Detailed Immunophenotyping of Peripheral Human Whole Blood Samples by Multicolor Flow Cytometry
por: Rühle, Paul F., et al.
Publicado: (2016)

High SARS-CoV-2 seroprevalence in health care workers but relatively low numbers of deaths in urban Malawi
por: Chibwana, Marah G., et al.
Publicado: (2020)

Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis
por: Marimuthu, Rekha, et al.
Publicado: (2018)

Imaging flow cytometry assays for quantifying pigment grade titanium dioxide particle internalization and interactions with immune cells in whole blood
por: Hewitt, Rachel E., et al.
Publicado: (2017)

Translating research flow cytometry assays for characterization of cellular therapeutics
por: Campbell, John DM
Publicado: (2015)

Flow Cytometry and Sorting
por: Dive, C.
Publicado: (1991)

Practical Flow Cytometry
por: Blair, Owen C.
Publicado: (1985)

A Flow Cytometry-Based Whole Blood Natural Killer Cell Cytotoxicity Assay Using Overnight Cytokine Activation
por: Kim, Jinho, et al.
Publicado: (2020)

Leveraging whole blood based functional flow cytometry assays to open new perspectives for rheumatoid arthritis translational research
por: Cartagena García, Celia, et al.
Publicado: (2022)

Virological failure, HIV-1 drug resistance, and early mortality in adults admitted to hospital in Malawi: an observational cohort study
por: Gupta-Wright, Ankur, et al.
Publicado: (2020)

Kinetics of Mycobacterium tuberculosis-specific IFN-γ responses and sputum bacillary clearance in HIV-infected adults during treatment of pulmonary tuberculosis
por: Mzinza, David T., et al.
Publicado: (2015)

A flow cytometry assay to quantify intercellular exchange of membrane components
por: Poulcharidis, Dimitrios, et al.
Publicado: (2017)

Flow cytometry assay for the detection of single-copy DNA in human lymphocytes
por: Uno, Naoki, et al.
Publicado: (2020)

Advanced Flow Cytometry Assays for Immune Monitoring of CAR-T Cell Applications
por: Blache, Ulrich, et al.
Publicado: (2021)

The in vitro micronucleus assay using imaging flow cytometry and deep learning
por: Rodrigues, Matthew A., et al.
Publicado: (2021)

Magnetic fingerprints of rolling cells for quantitative flow cytometry in whole blood
por: Reisbeck, Mathias, et al.
Publicado: (2016)

Cannot write session to /tmp/vufind_sessions/sess_d9bmf18mdsh36282el9lim6u2a