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Near-infrared luminescent metallacrowns for combined in vitro cell fixation and counter staining
Cell fixation is an essential approach for preserving cell morphology, allowing the targeting and labelling of biomolecules with fluorescent probes. One of the key requirements for more efficient fluorescent labelling is the preservation of cell morphology, which usually requires a combination of se...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Royal Society of Chemistry
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5625569/ https://www.ncbi.nlm.nih.gov/pubmed/28989634 http://dx.doi.org/10.1039/c7sc01872j |
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author | Martinić, Ivana Eliseeva, Svetlana V. Nguyen, Tu N. Foucher, Frédéric Gosset, David Westall, Frances Pecoraro, Vincent L. Petoud, Stéphane |
author_facet | Martinić, Ivana Eliseeva, Svetlana V. Nguyen, Tu N. Foucher, Frédéric Gosset, David Westall, Frances Pecoraro, Vincent L. Petoud, Stéphane |
author_sort | Martinić, Ivana |
collection | PubMed |
description | Cell fixation is an essential approach for preserving cell morphology, allowing the targeting and labelling of biomolecules with fluorescent probes. One of the key requirements for more efficient fluorescent labelling is the preservation of cell morphology, which usually requires a combination of several fixation techniques. In addition, the use of a counter stain is often essential to improve the contrast of the fluorescent probes. Current agents possess significant limitations, such as low resistance toward photobleaching and sensitivity to changes in the microenvironment. Luminescent Ln(3+) ‘encapsulated sandwich’ metallacrowns (MCs) overcome these drawbacks and offer complementary advantages. In particular, they emit sharp emission bands, possess a large difference between excitation and emission wavelengths and do not photobleach. Herein, MCs formed with pyrazinehydroxamic acid (Ln(3+)[Zn(ii)MC(pyzHA)], Ln(3+) = Yb, Nd) were used, combined with near-infrared (NIR) counter staining and fixation agents for HeLa cells upon an initial five minute exposure to UV-A light. The validity and quality of the cell fixation were assessed with Raman spectroscopy. Analysis of the NIR luminescence properties of these MCs was performed under different experimental conditions, including in a suspension of stained cells. Moreover, the high emission intensity of Ln(3+)[Zn(ii)MC(pyzHA)] in the NIR region allows these MCs to be used for imaging with standard CCD cameras installed on routine fluorescence microscopes. Finally, the NIR-emitting Ln(3+)[Zn(ii)MC(pyzHA)] compounds combine, within a single molecule, features such as cell fixation and staining abilities, good photostability and minimal sensitivity of the emission bands to the local microenvironment, and they are highly promising for establishing the next generation of imaging agents with a single biodistribution. |
format | Online Article Text |
id | pubmed-5625569 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-56255692017-10-06 Near-infrared luminescent metallacrowns for combined in vitro cell fixation and counter staining Martinić, Ivana Eliseeva, Svetlana V. Nguyen, Tu N. Foucher, Frédéric Gosset, David Westall, Frances Pecoraro, Vincent L. Petoud, Stéphane Chem Sci Chemistry Cell fixation is an essential approach for preserving cell morphology, allowing the targeting and labelling of biomolecules with fluorescent probes. One of the key requirements for more efficient fluorescent labelling is the preservation of cell morphology, which usually requires a combination of several fixation techniques. In addition, the use of a counter stain is often essential to improve the contrast of the fluorescent probes. Current agents possess significant limitations, such as low resistance toward photobleaching and sensitivity to changes in the microenvironment. Luminescent Ln(3+) ‘encapsulated sandwich’ metallacrowns (MCs) overcome these drawbacks and offer complementary advantages. In particular, they emit sharp emission bands, possess a large difference between excitation and emission wavelengths and do not photobleach. Herein, MCs formed with pyrazinehydroxamic acid (Ln(3+)[Zn(ii)MC(pyzHA)], Ln(3+) = Yb, Nd) were used, combined with near-infrared (NIR) counter staining and fixation agents for HeLa cells upon an initial five minute exposure to UV-A light. The validity and quality of the cell fixation were assessed with Raman spectroscopy. Analysis of the NIR luminescence properties of these MCs was performed under different experimental conditions, including in a suspension of stained cells. Moreover, the high emission intensity of Ln(3+)[Zn(ii)MC(pyzHA)] in the NIR region allows these MCs to be used for imaging with standard CCD cameras installed on routine fluorescence microscopes. Finally, the NIR-emitting Ln(3+)[Zn(ii)MC(pyzHA)] compounds combine, within a single molecule, features such as cell fixation and staining abilities, good photostability and minimal sensitivity of the emission bands to the local microenvironment, and they are highly promising for establishing the next generation of imaging agents with a single biodistribution. Royal Society of Chemistry 2017-09-01 2017-08-08 /pmc/articles/PMC5625569/ /pubmed/28989634 http://dx.doi.org/10.1039/c7sc01872j Text en This journal is © The Royal Society of Chemistry 2017 http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution 3.0 Unported License (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Chemistry Martinić, Ivana Eliseeva, Svetlana V. Nguyen, Tu N. Foucher, Frédéric Gosset, David Westall, Frances Pecoraro, Vincent L. Petoud, Stéphane Near-infrared luminescent metallacrowns for combined in vitro cell fixation and counter staining |
title | Near-infrared luminescent metallacrowns for combined in vitro cell fixation and counter staining
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title_full | Near-infrared luminescent metallacrowns for combined in vitro cell fixation and counter staining
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title_fullStr | Near-infrared luminescent metallacrowns for combined in vitro cell fixation and counter staining
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title_full_unstemmed | Near-infrared luminescent metallacrowns for combined in vitro cell fixation and counter staining
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title_short | Near-infrared luminescent metallacrowns for combined in vitro cell fixation and counter staining
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title_sort | near-infrared luminescent metallacrowns for combined in vitro cell fixation and counter staining |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5625569/ https://www.ncbi.nlm.nih.gov/pubmed/28989634 http://dx.doi.org/10.1039/c7sc01872j |
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