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Filopodyan: An open-source pipeline for the analysis of filopodia
Filopodia have important sensory and mechanical roles in motile cells. The recruitment of actin regulators, such as ENA/VASP proteins, to sites of protrusion underlies diverse molecular mechanisms of filopodia formation and extension. We developed Filopodyan (filopodia dynamics analysis) in Fiji and...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5626553/ https://www.ncbi.nlm.nih.gov/pubmed/28760769 http://dx.doi.org/10.1083/jcb.201705113 |
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author | Urbančič, Vasja Butler, Richard Richier, Benjamin Peter, Manuel Mason, Julia Livesey, Frederick J. Holt, Christine E. Gallop, Jennifer L. |
author_facet | Urbančič, Vasja Butler, Richard Richier, Benjamin Peter, Manuel Mason, Julia Livesey, Frederick J. Holt, Christine E. Gallop, Jennifer L. |
author_sort | Urbančič, Vasja |
collection | PubMed |
description | Filopodia have important sensory and mechanical roles in motile cells. The recruitment of actin regulators, such as ENA/VASP proteins, to sites of protrusion underlies diverse molecular mechanisms of filopodia formation and extension. We developed Filopodyan (filopodia dynamics analysis) in Fiji and R to measure fluorescence in filopodia and at their tips and bases concurrently with their morphological and dynamic properties. Filopodyan supports high-throughput phenotype characterization as well as detailed interactive editing of filopodia reconstructions through an intuitive graphical user interface. Our highly customizable pipeline is widely applicable, capable of detecting filopodia in four different cell types in vitro and in vivo. We use Filopodyan to quantify the recruitment of ENA and VASP preceding filopodia formation in neuronal growth cones, and uncover a molecular heterogeneity whereby different filopodia display markedly different responses to changes in the accumulation of ENA and VASP fluorescence in their tips over time. |
format | Online Article Text |
id | pubmed-5626553 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-56265532017-10-05 Filopodyan: An open-source pipeline for the analysis of filopodia Urbančič, Vasja Butler, Richard Richier, Benjamin Peter, Manuel Mason, Julia Livesey, Frederick J. Holt, Christine E. Gallop, Jennifer L. J Cell Biol Research Articles Filopodia have important sensory and mechanical roles in motile cells. The recruitment of actin regulators, such as ENA/VASP proteins, to sites of protrusion underlies diverse molecular mechanisms of filopodia formation and extension. We developed Filopodyan (filopodia dynamics analysis) in Fiji and R to measure fluorescence in filopodia and at their tips and bases concurrently with their morphological and dynamic properties. Filopodyan supports high-throughput phenotype characterization as well as detailed interactive editing of filopodia reconstructions through an intuitive graphical user interface. Our highly customizable pipeline is widely applicable, capable of detecting filopodia in four different cell types in vitro and in vivo. We use Filopodyan to quantify the recruitment of ENA and VASP preceding filopodia formation in neuronal growth cones, and uncover a molecular heterogeneity whereby different filopodia display markedly different responses to changes in the accumulation of ENA and VASP fluorescence in their tips over time. The Rockefeller University Press 2017-10-02 /pmc/articles/PMC5626553/ /pubmed/28760769 http://dx.doi.org/10.1083/jcb.201705113 Text en © 2017 Urbančič et al. https://creativecommons.org/licenses/by/4.0/This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Research Articles Urbančič, Vasja Butler, Richard Richier, Benjamin Peter, Manuel Mason, Julia Livesey, Frederick J. Holt, Christine E. Gallop, Jennifer L. Filopodyan: An open-source pipeline for the analysis of filopodia |
title | Filopodyan: An open-source pipeline for the analysis of filopodia |
title_full | Filopodyan: An open-source pipeline for the analysis of filopodia |
title_fullStr | Filopodyan: An open-source pipeline for the analysis of filopodia |
title_full_unstemmed | Filopodyan: An open-source pipeline for the analysis of filopodia |
title_short | Filopodyan: An open-source pipeline for the analysis of filopodia |
title_sort | filopodyan: an open-source pipeline for the analysis of filopodia |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5626553/ https://www.ncbi.nlm.nih.gov/pubmed/28760769 http://dx.doi.org/10.1083/jcb.201705113 |
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