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Motionless volumetric photoacoustic microscopy with spatially invariant resolution
Photoacoustic microscopy (PAM) is uniquely positioned for biomedical applications because of its ability to visualize optical absorption contrast in vivo in three dimensions. Here we propose motionless volumetric spatially invariant resolution photoacoustic microscopy (SIR-PAM). To realize motionles...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5626698/ https://www.ncbi.nlm.nih.gov/pubmed/28974681 http://dx.doi.org/10.1038/s41467-017-00856-2 |
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author | Yang, Jiamiao Gong, Lei Xu, Xiao Hai, Pengfei Shen, Yuecheng Suzuki, Yuta Wang, Lihong V. |
author_facet | Yang, Jiamiao Gong, Lei Xu, Xiao Hai, Pengfei Shen, Yuecheng Suzuki, Yuta Wang, Lihong V. |
author_sort | Yang, Jiamiao |
collection | PubMed |
description | Photoacoustic microscopy (PAM) is uniquely positioned for biomedical applications because of its ability to visualize optical absorption contrast in vivo in three dimensions. Here we propose motionless volumetric spatially invariant resolution photoacoustic microscopy (SIR-PAM). To realize motionless volumetric imaging, SIR-PAM combines two-dimensional Fourier-spectrum optical excitation with single-element depth-resolved photoacoustic detection. To achieve spatially invariant lateral resolution, propagation-invariant sinusoidal fringes are generated by a digital micromirror device. Further, SIR-PAM achieves 1.5 times finer lateral resolution than conventional PAM. The superior performance was demonstrated in imaging both inanimate objects and animals in vivo with a resolution-invariant axial range of 1.8 mm, 33 times the depth of field of the conventional PAM counterpart. Our work opens new perspectives for PAM in biomedical sciences. |
format | Online Article Text |
id | pubmed-5626698 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-56266982017-10-05 Motionless volumetric photoacoustic microscopy with spatially invariant resolution Yang, Jiamiao Gong, Lei Xu, Xiao Hai, Pengfei Shen, Yuecheng Suzuki, Yuta Wang, Lihong V. Nat Commun Article Photoacoustic microscopy (PAM) is uniquely positioned for biomedical applications because of its ability to visualize optical absorption contrast in vivo in three dimensions. Here we propose motionless volumetric spatially invariant resolution photoacoustic microscopy (SIR-PAM). To realize motionless volumetric imaging, SIR-PAM combines two-dimensional Fourier-spectrum optical excitation with single-element depth-resolved photoacoustic detection. To achieve spatially invariant lateral resolution, propagation-invariant sinusoidal fringes are generated by a digital micromirror device. Further, SIR-PAM achieves 1.5 times finer lateral resolution than conventional PAM. The superior performance was demonstrated in imaging both inanimate objects and animals in vivo with a resolution-invariant axial range of 1.8 mm, 33 times the depth of field of the conventional PAM counterpart. Our work opens new perspectives for PAM in biomedical sciences. Nature Publishing Group UK 2017-10-03 /pmc/articles/PMC5626698/ /pubmed/28974681 http://dx.doi.org/10.1038/s41467-017-00856-2 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Yang, Jiamiao Gong, Lei Xu, Xiao Hai, Pengfei Shen, Yuecheng Suzuki, Yuta Wang, Lihong V. Motionless volumetric photoacoustic microscopy with spatially invariant resolution |
title | Motionless volumetric photoacoustic microscopy with spatially invariant resolution |
title_full | Motionless volumetric photoacoustic microscopy with spatially invariant resolution |
title_fullStr | Motionless volumetric photoacoustic microscopy with spatially invariant resolution |
title_full_unstemmed | Motionless volumetric photoacoustic microscopy with spatially invariant resolution |
title_short | Motionless volumetric photoacoustic microscopy with spatially invariant resolution |
title_sort | motionless volumetric photoacoustic microscopy with spatially invariant resolution |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5626698/ https://www.ncbi.nlm.nih.gov/pubmed/28974681 http://dx.doi.org/10.1038/s41467-017-00856-2 |
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