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Spontaneous charged lipid transfer between lipid vesicles

An assay to study the spontaneous charged lipid transfer between lipid vesicles is described. A donor/acceptor vesicle system is employed, where neutrally charged acceptor vesicles are fluorescently labelled with the electrostatic membrane probe Fluoresceinphosphatidylethanolamine (FPE). Upon additi...

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Autores principales: Richens, Joanna L., Tyler, Arwen I. I., Barriga, Hanna M. G., Bramble, Jonathan P., Law, Robert V., Brooks, Nicholas J., Seddon, John M., Ces, Oscar, O’Shea, Paul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5626757/
https://www.ncbi.nlm.nih.gov/pubmed/28974701
http://dx.doi.org/10.1038/s41598-017-12611-0
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author Richens, Joanna L.
Tyler, Arwen I. I.
Barriga, Hanna M. G.
Bramble, Jonathan P.
Law, Robert V.
Brooks, Nicholas J.
Seddon, John M.
Ces, Oscar
O’Shea, Paul
author_facet Richens, Joanna L.
Tyler, Arwen I. I.
Barriga, Hanna M. G.
Bramble, Jonathan P.
Law, Robert V.
Brooks, Nicholas J.
Seddon, John M.
Ces, Oscar
O’Shea, Paul
author_sort Richens, Joanna L.
collection PubMed
description An assay to study the spontaneous charged lipid transfer between lipid vesicles is described. A donor/acceptor vesicle system is employed, where neutrally charged acceptor vesicles are fluorescently labelled with the electrostatic membrane probe Fluoresceinphosphatidylethanolamine (FPE). Upon addition of charged donor vesicles, transfer of negatively charged lipid occurs, resulting in a fluorescently detectable change in the membrane potential of the acceptor vesicles. Using this approach we have studied the transfer properties of a range of lipids, varying both the headgroup and the chain length. At the low vesicle concentrations chosen, the transfer follows a first-order process where lipid monomers are transferred presumably through the aqueous solution phase from donor to acceptor vesicle. The rate of transfer decreases with increasing chain length which is consistent with energy models previously reported for lipid monomer vesicle interactions. Our assay improves on existing methods allowing the study of a range of unmodified lipids, continuous monitoring of transfer and simplified experimental procedures.
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spelling pubmed-56267572017-10-12 Spontaneous charged lipid transfer between lipid vesicles Richens, Joanna L. Tyler, Arwen I. I. Barriga, Hanna M. G. Bramble, Jonathan P. Law, Robert V. Brooks, Nicholas J. Seddon, John M. Ces, Oscar O’Shea, Paul Sci Rep Article An assay to study the spontaneous charged lipid transfer between lipid vesicles is described. A donor/acceptor vesicle system is employed, where neutrally charged acceptor vesicles are fluorescently labelled with the electrostatic membrane probe Fluoresceinphosphatidylethanolamine (FPE). Upon addition of charged donor vesicles, transfer of negatively charged lipid occurs, resulting in a fluorescently detectable change in the membrane potential of the acceptor vesicles. Using this approach we have studied the transfer properties of a range of lipids, varying both the headgroup and the chain length. At the low vesicle concentrations chosen, the transfer follows a first-order process where lipid monomers are transferred presumably through the aqueous solution phase from donor to acceptor vesicle. The rate of transfer decreases with increasing chain length which is consistent with energy models previously reported for lipid monomer vesicle interactions. Our assay improves on existing methods allowing the study of a range of unmodified lipids, continuous monitoring of transfer and simplified experimental procedures. Nature Publishing Group UK 2017-10-03 /pmc/articles/PMC5626757/ /pubmed/28974701 http://dx.doi.org/10.1038/s41598-017-12611-0 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Richens, Joanna L.
Tyler, Arwen I. I.
Barriga, Hanna M. G.
Bramble, Jonathan P.
Law, Robert V.
Brooks, Nicholas J.
Seddon, John M.
Ces, Oscar
O’Shea, Paul
Spontaneous charged lipid transfer between lipid vesicles
title Spontaneous charged lipid transfer between lipid vesicles
title_full Spontaneous charged lipid transfer between lipid vesicles
title_fullStr Spontaneous charged lipid transfer between lipid vesicles
title_full_unstemmed Spontaneous charged lipid transfer between lipid vesicles
title_short Spontaneous charged lipid transfer between lipid vesicles
title_sort spontaneous charged lipid transfer between lipid vesicles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5626757/
https://www.ncbi.nlm.nih.gov/pubmed/28974701
http://dx.doi.org/10.1038/s41598-017-12611-0
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