Cargando…

In Vivo Knockout of the Vegfa Gene by Lentiviral Delivery of CRISPR/Cas9 in Mouse Retinal Pigment Epithelium Cells

Virus-based gene therapy by CRISPR/Cas9-mediated genome editing and knockout may provide a new option for treatment of inherited and acquired ocular diseases of the retina. In support of this notion, we show that Streptococcus pyogenes (Sp) Cas9, delivered by lentiviral vectors (LVs), can be used in...

Descripción completa

Detalles Bibliográficos
Autores principales:	Holmgaard, Andreas, Askou, Anne Louise, Benckendorff, Josephine Natalia Esther, Thomsen, Emil Aagaard, Cai, Yujia, Bek, Toke, Mikkelsen, Jacob Giehm, Corydon, Thomas J.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Society of Gene & Cell Therapy 2017
Materias:	Original Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5626917/ https://www.ncbi.nlm.nih.gov/pubmed/29246327 http://dx.doi.org/10.1016/j.omtn.2017.08.016

Ejemplares similares

Suppression of Choroidal Neovascularization by AAV-Based Dual-Acting Antiangiogenic Gene Therapy
por: Askou, Anne Louise, et al.
Publicado: (2019)

Targeted Knockout of the Vegfa Gene in the Retina by Subretinal Injection of RNP Complexes Containing Cas9 Protein and Modified sgRNAs
por: Holmgaard, Andreas Braae, et al.
Publicado: (2021)

Multigenic lentiviral vectors for combined and tissue-specific expression of miRNA- and protein-based antiangiogenic factors
por: Askou, Anne Louise, et al.
Publicado: (2015)

CRISPR-Cas9-directed gene tagging using a single integrase-defective lentiviral vector carrying a transposase-based Cas9 off switch
por: Thomsen, Emil Aagaard, et al.
Publicado: (2022)

Improved Lentiviral Gene Delivery to Mouse Liver by Hydrodynamic Vector Injection through Tail Vein
por: Dalsgaard, Trine, et al.
Publicado: (2018)

Driving DNA transposition by lentiviral protein transduction
por: Cai, Yujia, et al.
Publicado: (2014)

Toward lentiviral vectors for antiangiogenic ocular gene therapy
por: Askou, Anne Louise, et al.
Publicado: (2023)

Engineered lentivirus-derived nanoparticles (LVNPs) for delivery of CRISPR/Cas ribonucleoprotein complexes supporting base editing, prime editing and in vivo gene modification
por: Haldrup, Jakob, et al.
Publicado: (2023)

Subretinal Saline Protects the Neuroretina From Thermic Damage During Laser Induction of Experimental Choroidal Neovascularization in Pigs
por: Hansen, Silja, et al.
Publicado: (2021)

DNA transposition by protein transduction of the piggyBac transposase from lentiviral Gag precursors
por: Cai, Yujia, et al.
Publicado: (2014)

Targeted genome editing by lentiviral protein transduction of zinc-finger and TAL-effector nucleases
por: Cai, Yujia, et al.
Publicado: (2014)

VEGFA-targeting miR-agshRNAs combine efficacy with specificity and safety for retinal gene therapy
por: Alsing, Sidsel, et al.
Publicado: (2022)

CRISPR/Cas9 mediated specific ablation of vegfa in retinal pigment epithelium efficiently regresses choroidal neovascularization
por: Park, Jinkyu, et al.
Publicado: (2023)

Animal Models of Choroidal Neovascularization: A Systematic Review
por: Fabian-Jessing, Bjørn K., et al.
Publicado: (2022)

Identification of BLNK and BTK as mediators of rituximab‐induced programmed cell death by CRISPR screens in GCB‐subtype diffuse large B‐cell lymphoma
por: Thomsen, Emil Aagaard, et al.
Publicado: (2020)

Sortilin Inhibition Protects Neurons From Degeneration in the Diabetic Retina
por: Jakobsen, Thomas Stax, et al.
Publicado: (2023)

piggyPrime: High-Efficacy Prime Editing in Human Cells Using piggyBac-Based DNA Transposition
por: Wolff, Jonas Holst, et al.
Publicado: (2021)

pegIT - a web-based design tool for prime editing
por: Anderson, Mads Valdemar, et al.
Publicado: (2021)

The Impact of cHS4 Insulators on DNA Transposon Vector Mobilization and Silencing in Retinal Pigment Epithelium Cells
por: Sharma, Nynne, et al.
Publicado: (2012)

Targeted, homology-driven gene insertion in stem cells by ZFN-loaded ‘all-in-one’ lentiviral vectors
por: Cai, Yujia, et al.
Publicado: (2016)

Single-Cell Monitoring of Activated Innate Immune Signaling by a d2eGFP-Based Reporter Mimicking Time-Restricted Activation of IFNB1 Expression
por: Thomsen, Emil Aagaard, et al.
Publicado: (2022)

Prime editing in hematopoietic stem cells—From ex vivo to in vivo CRISPR-based treatment of blood disorders
por: Wolff, Jonas Holst, et al.
Publicado: (2023)

CRISPR-screen identifies ZIP9 and dysregulated Zn(2+) homeostasis as a cause of cancer-associated changes in glycosylation
por: Rømer, Troels Boldt, et al.
Publicado: (2023)

Anti-Apoptotic Effects of Lentiviral Vector Transduction Promote Increased Rituximab Tolerance in Cancerous B-Cells
por: Ranjbar, Benyamin, et al.
Publicado: (2016)

α-Crystallin distribution in retinal pigment epithelium and effect of gene knockouts on sensitivity to oxidative stress
por: Yaung, Jennifer, et al.
Publicado: (2007)

25 years of maturation: A systematic review of RNAi in the clinic
por: Corydon, Ida Juhl, et al.
Publicado: (2023)

Lentiviral CRISPR-guided RNA library screening identified Adam17 as an upstream negative regulator of Procr in mammary epithelium
por: Wu, Ting, et al.
Publicado: (2021)

Lentiviral in situ targeting of stem cells in unperturbed intestinal epithelium
por: Garside, George B., et al.
Publicado: (2023)

CRISPR-mediated knockout of cardinal and cinnabar eye pigmentation genes in the western tarnished plant bug
por: Heu, Chan C., et al.
Publicado: (2022)

Establishment of a CRISPR/Cas9-Mediated Efficient Knockout System of Trichoderma hamatum T21 and Pigment Synthesis PKS Gene Knockout
por: Luo, Ning, et al.
Publicado: (2023)

Protrusion of KCNJ13 Gene Knockout Retinal Pigment Epithelium Due to Oxidative Stress–Induced Cell Death
por: Kanzaki, Yuki, et al.
Publicado: (2022)

Bestrophin 2 is expressed in human non-pigmented ciliary epithelium but not retinal pigment epithelium
por: Zhang, Youwen, et al.
Publicado: (2010)

Evaluation of focal damage in the retinal pigment epithelium layer in serous retinal pigment epithelium detachment
por: Miura, Masahiro, et al.
Publicado: (2019)

Protocol for in vivo CRISPR screening using selective CRISPR antigen removal lentiviral vectors
por: Lane-Reticker, Sarah Kate, et al.
Publicado: (2023)

Translocation of the retinal pigment epithelium and formation of sub-retinal pigment epithelium deposit induced by subretinal deposit
por: Zhao, Lian, et al.
Publicado: (2007)

Adenoma of the Ciliary Pigment Epithelium with Diffuse Iris Pigment Dispersion
por: Chang, Ying, et al.
Publicado: (2015)

Local Adenoviral Delivery of Vascular Endothelial Growth Factor C Induces Lymphangiogenesis in the Conjunctiva in Rabbits
por: Rasic, Dusan, et al.
Publicado: (2023)

Setting Eyes on the Retinal Pigment Epithelium
por: Moreno-Marmol, Tania, et al.
Publicado: (2018)

Functions and Diseases of the Retinal Pigment Epithelium
por: Yang, Song, et al.
Publicado: (2021)

Improved microRNA suppression by WPRE-linked tough decoy microRNA sponges
por: Hollensen, Anne Kruse, et al.
Publicado: (2017)

Cannot write session to /tmp/vufind_sessions/sess_t68hi9at0332lkjiufidk49a3i