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Preliminary molecular characterization of a proinflammatory and nociceptive molecule from the Echinometra lucunter spines extracts

BACKGROUND: Sea urchins are animals commonly found on the Brazilian shoreline, being Echinometra lucunter the most abundant species. Accidents caused by E. lucunter have been reported as one of the most frequent in Brazil, and are characterized by intense pain and inflammation, consequence of spine...

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Autores principales: Sciani, Juliana Mozer, Zychar, Bianca, Gonçalves, Luis Roberto, Giorgi, Renata, Nogueira, Thiago, Pimenta, Daniel Carvalho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5627400/
https://www.ncbi.nlm.nih.gov/pubmed/29026370
http://dx.doi.org/10.1186/s40409-017-0133-8
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author Sciani, Juliana Mozer
Zychar, Bianca
Gonçalves, Luis Roberto
Giorgi, Renata
Nogueira, Thiago
Pimenta, Daniel Carvalho
author_facet Sciani, Juliana Mozer
Zychar, Bianca
Gonçalves, Luis Roberto
Giorgi, Renata
Nogueira, Thiago
Pimenta, Daniel Carvalho
author_sort Sciani, Juliana Mozer
collection PubMed
description BACKGROUND: Sea urchins are animals commonly found on the Brazilian shoreline, being Echinometra lucunter the most abundant species. Accidents caused by E. lucunter have been reported as one of the most frequent in Brazil, and are characterized by intense pain and inflammation, consequence of spine puncture in the skin. In order to characterize such toxic effects, we isolated one molecule that caused inflammatory and nociceptive effects. METHODS: E. lucunter specimens were collected without gender distinction. Spines were removed and molecules were extracted, fractionated by RP-HPLC and assayed for inflammatory and nociceptive activity, in a biological-driven fractionation way, until the obtainment of one active molecule and its subsequent analysis by mass spectrometry (MS and MS/MS). For inflammation, intravital microscopy was performed on the mouse cremaster muscle, in order to evaluate rolled, adherent and migrating leukocytes. Paw edema was also evaluated. For the nociceptive activity, the paw pressure test was performed in rats. RESULTS: One molecule could be isolated and related to the inflammatory and nociceptive activity. Regarding inflammation, increase in adherent and migrating cells was observed in the cremaster muscle after the administration of the molecule. Corroborating the inflammatory response, paw edema was also observed, although only in 20% of controls and 20 min after injection. Additionally, this molecule was able to decrease significantly the pain threshold, characterizing hyperalgesia. This molecule was analyzed by mass spectrometry, and according to the exact molecular mass, isotopic distribution and fragmentation profile, it was possible to propose the molecular formula C(29)H(48)N(3)O(10). CONCLUSIONS: One isolated molecule from the spine extract of E. lucunter is able to elicit inflammation and hypernociception in animal models, which is in agreement with the effects observed in sea urchin accidents.
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spelling pubmed-56274002017-10-12 Preliminary molecular characterization of a proinflammatory and nociceptive molecule from the Echinometra lucunter spines extracts Sciani, Juliana Mozer Zychar, Bianca Gonçalves, Luis Roberto Giorgi, Renata Nogueira, Thiago Pimenta, Daniel Carvalho J Venom Anim Toxins Incl Trop Dis Research BACKGROUND: Sea urchins are animals commonly found on the Brazilian shoreline, being Echinometra lucunter the most abundant species. Accidents caused by E. lucunter have been reported as one of the most frequent in Brazil, and are characterized by intense pain and inflammation, consequence of spine puncture in the skin. In order to characterize such toxic effects, we isolated one molecule that caused inflammatory and nociceptive effects. METHODS: E. lucunter specimens were collected without gender distinction. Spines were removed and molecules were extracted, fractionated by RP-HPLC and assayed for inflammatory and nociceptive activity, in a biological-driven fractionation way, until the obtainment of one active molecule and its subsequent analysis by mass spectrometry (MS and MS/MS). For inflammation, intravital microscopy was performed on the mouse cremaster muscle, in order to evaluate rolled, adherent and migrating leukocytes. Paw edema was also evaluated. For the nociceptive activity, the paw pressure test was performed in rats. RESULTS: One molecule could be isolated and related to the inflammatory and nociceptive activity. Regarding inflammation, increase in adherent and migrating cells was observed in the cremaster muscle after the administration of the molecule. Corroborating the inflammatory response, paw edema was also observed, although only in 20% of controls and 20 min after injection. Additionally, this molecule was able to decrease significantly the pain threshold, characterizing hyperalgesia. This molecule was analyzed by mass spectrometry, and according to the exact molecular mass, isotopic distribution and fragmentation profile, it was possible to propose the molecular formula C(29)H(48)N(3)O(10). CONCLUSIONS: One isolated molecule from the spine extract of E. lucunter is able to elicit inflammation and hypernociception in animal models, which is in agreement with the effects observed in sea urchin accidents. BioMed Central 2017-10-03 /pmc/articles/PMC5627400/ /pubmed/29026370 http://dx.doi.org/10.1186/s40409-017-0133-8 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Sciani, Juliana Mozer
Zychar, Bianca
Gonçalves, Luis Roberto
Giorgi, Renata
Nogueira, Thiago
Pimenta, Daniel Carvalho
Preliminary molecular characterization of a proinflammatory and nociceptive molecule from the Echinometra lucunter spines extracts
title Preliminary molecular characterization of a proinflammatory and nociceptive molecule from the Echinometra lucunter spines extracts
title_full Preliminary molecular characterization of a proinflammatory and nociceptive molecule from the Echinometra lucunter spines extracts
title_fullStr Preliminary molecular characterization of a proinflammatory and nociceptive molecule from the Echinometra lucunter spines extracts
title_full_unstemmed Preliminary molecular characterization of a proinflammatory and nociceptive molecule from the Echinometra lucunter spines extracts
title_short Preliminary molecular characterization of a proinflammatory and nociceptive molecule from the Echinometra lucunter spines extracts
title_sort preliminary molecular characterization of a proinflammatory and nociceptive molecule from the echinometra lucunter spines extracts
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5627400/
https://www.ncbi.nlm.nih.gov/pubmed/29026370
http://dx.doi.org/10.1186/s40409-017-0133-8
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