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Methods for genetic transformation of filamentous fungi

Filamentous fungi have been of great interest because of their excellent ability as cell factories to manufacture useful products for human beings. The development of genetic transformation techniques is a precondition that enables scientists to target and modify genes efficiently and may reveal the...

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Detalles Bibliográficos
Autores principales: Li, Dandan, Tang, Yu, Lin, Jun, Cai, Weiwen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5627406/
https://www.ncbi.nlm.nih.gov/pubmed/28974205
http://dx.doi.org/10.1186/s12934-017-0785-7
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author Li, Dandan
Tang, Yu
Lin, Jun
Cai, Weiwen
author_facet Li, Dandan
Tang, Yu
Lin, Jun
Cai, Weiwen
author_sort Li, Dandan
collection PubMed
description Filamentous fungi have been of great interest because of their excellent ability as cell factories to manufacture useful products for human beings. The development of genetic transformation techniques is a precondition that enables scientists to target and modify genes efficiently and may reveal the function of target genes. The method to deliver foreign nucleic acid into cells is the sticking point for fungal genome modification. Up to date, there are some general methods of genetic transformation for fungi, including protoplast-mediated transformation, Agrobacterium-mediated transformation, electroporation, biolistic method and shock-wave-mediated transformation. This article reviews basic protocols and principles of these transformation methods, as well as their advantages and disadvantages.
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spelling pubmed-56274062017-10-12 Methods for genetic transformation of filamentous fungi Li, Dandan Tang, Yu Lin, Jun Cai, Weiwen Microb Cell Fact Review Filamentous fungi have been of great interest because of their excellent ability as cell factories to manufacture useful products for human beings. The development of genetic transformation techniques is a precondition that enables scientists to target and modify genes efficiently and may reveal the function of target genes. The method to deliver foreign nucleic acid into cells is the sticking point for fungal genome modification. Up to date, there are some general methods of genetic transformation for fungi, including protoplast-mediated transformation, Agrobacterium-mediated transformation, electroporation, biolistic method and shock-wave-mediated transformation. This article reviews basic protocols and principles of these transformation methods, as well as their advantages and disadvantages. BioMed Central 2017-10-03 /pmc/articles/PMC5627406/ /pubmed/28974205 http://dx.doi.org/10.1186/s12934-017-0785-7 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Review
Li, Dandan
Tang, Yu
Lin, Jun
Cai, Weiwen
Methods for genetic transformation of filamentous fungi
title Methods for genetic transformation of filamentous fungi
title_full Methods for genetic transformation of filamentous fungi
title_fullStr Methods for genetic transformation of filamentous fungi
title_full_unstemmed Methods for genetic transformation of filamentous fungi
title_short Methods for genetic transformation of filamentous fungi
title_sort methods for genetic transformation of filamentous fungi
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5627406/
https://www.ncbi.nlm.nih.gov/pubmed/28974205
http://dx.doi.org/10.1186/s12934-017-0785-7
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