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Negative pressure wound therapy: Regulating blood flow perfusion and microvessel maturation through microvascular pericytes

Negative pressure wound therapy (NPWT) has been demonstrated to accelerate wound healing by promoting angiogenesis. However, whether blood flow perfusion is regulated by microvessel maturation and pericytes following NPWT remains unclear, as well as the exact association between pericytes and collag...

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Autores principales: Ma, Zhanjun, Li, Zonghuan, Shou, Kangquan, Jian, Chao, Li, Pengcheng, Niu, Yahui, Qi, Baiwen, Yu, Aixi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5627868/
https://www.ncbi.nlm.nih.gov/pubmed/28901392
http://dx.doi.org/10.3892/ijmm.2017.3131
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author Ma, Zhanjun
Li, Zonghuan
Shou, Kangquan
Jian, Chao
Li, Pengcheng
Niu, Yahui
Qi, Baiwen
Yu, Aixi
author_facet Ma, Zhanjun
Li, Zonghuan
Shou, Kangquan
Jian, Chao
Li, Pengcheng
Niu, Yahui
Qi, Baiwen
Yu, Aixi
author_sort Ma, Zhanjun
collection PubMed
description Negative pressure wound therapy (NPWT) has been demonstrated to accelerate wound healing by promoting angiogenesis. However, whether blood flow perfusion is regulated by microvessel maturation and pericytes following NPWT remains unclear, as well as the exact association between pericytes and collagen type IV. The aim of this study was to investigate the relevant association between blood flow perfusion and microvessel maturation and pericytes following NPWT, and to further explore the underlying molecular mechanisms. We also aimed to investigate the association between pericytes and collagen type IV. For this purpose, we created a rat model of diabetic wounds and microvascular blood flow perfusion was detected using a laser Doppler blood perfusion imager. The expression levels of angiogenin-1, tyrosine phosphorylation of tyrosine kinase receptor-2 (Tie-2), α-smooth muscle actin (α-SMA) and collagen type IV were detected and analyzed through immunohistochemistry, immunofluorescence, RT-qPCR and western blot analysis. The results revealed that NPWT promoted the overexpression of angiogenin-1, Tie-2, α-SMA and collagen type IV, and significantly increased blood flow perfusion coupled with microvessel maturation in the NPWT group at the later stages (7–10 days) of wound healing. Our results suggested that NPWT can preferentially enhance vessel maturation and increase the number of pericytes, thus regulating blood flow perfusion. On the other hand, pericytes and collagen type IV had a mutual interaction, promoting microvessel maturation.
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spelling pubmed-56278682017-10-08 Negative pressure wound therapy: Regulating blood flow perfusion and microvessel maturation through microvascular pericytes Ma, Zhanjun Li, Zonghuan Shou, Kangquan Jian, Chao Li, Pengcheng Niu, Yahui Qi, Baiwen Yu, Aixi Int J Mol Med Articles Negative pressure wound therapy (NPWT) has been demonstrated to accelerate wound healing by promoting angiogenesis. However, whether blood flow perfusion is regulated by microvessel maturation and pericytes following NPWT remains unclear, as well as the exact association between pericytes and collagen type IV. The aim of this study was to investigate the relevant association between blood flow perfusion and microvessel maturation and pericytes following NPWT, and to further explore the underlying molecular mechanisms. We also aimed to investigate the association between pericytes and collagen type IV. For this purpose, we created a rat model of diabetic wounds and microvascular blood flow perfusion was detected using a laser Doppler blood perfusion imager. The expression levels of angiogenin-1, tyrosine phosphorylation of tyrosine kinase receptor-2 (Tie-2), α-smooth muscle actin (α-SMA) and collagen type IV were detected and analyzed through immunohistochemistry, immunofluorescence, RT-qPCR and western blot analysis. The results revealed that NPWT promoted the overexpression of angiogenin-1, Tie-2, α-SMA and collagen type IV, and significantly increased blood flow perfusion coupled with microvessel maturation in the NPWT group at the later stages (7–10 days) of wound healing. Our results suggested that NPWT can preferentially enhance vessel maturation and increase the number of pericytes, thus regulating blood flow perfusion. On the other hand, pericytes and collagen type IV had a mutual interaction, promoting microvessel maturation. D.A. Spandidos 2017-11 2017-09-13 /pmc/articles/PMC5627868/ /pubmed/28901392 http://dx.doi.org/10.3892/ijmm.2017.3131 Text en Copyright: © Ma et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Ma, Zhanjun
Li, Zonghuan
Shou, Kangquan
Jian, Chao
Li, Pengcheng
Niu, Yahui
Qi, Baiwen
Yu, Aixi
Negative pressure wound therapy: Regulating blood flow perfusion and microvessel maturation through microvascular pericytes
title Negative pressure wound therapy: Regulating blood flow perfusion and microvessel maturation through microvascular pericytes
title_full Negative pressure wound therapy: Regulating blood flow perfusion and microvessel maturation through microvascular pericytes
title_fullStr Negative pressure wound therapy: Regulating blood flow perfusion and microvessel maturation through microvascular pericytes
title_full_unstemmed Negative pressure wound therapy: Regulating blood flow perfusion and microvessel maturation through microvascular pericytes
title_short Negative pressure wound therapy: Regulating blood flow perfusion and microvessel maturation through microvascular pericytes
title_sort negative pressure wound therapy: regulating blood flow perfusion and microvessel maturation through microvascular pericytes
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5627868/
https://www.ncbi.nlm.nih.gov/pubmed/28901392
http://dx.doi.org/10.3892/ijmm.2017.3131
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