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Impaired polyfunctionality of CD8(+) T cells in severe sepsis patients with human cytomegalovirus reactivation
Human cytomegalovirus (HCMV) establishes a lifelong chronic latent infection and often reactivates in immunocompromised patients. In addition, HCMV reactivates in patients with sepsis or other critical illnesses, particularly in patients with poor prognoses. However, the immunological characteristic...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5628278/ https://www.ncbi.nlm.nih.gov/pubmed/28960213 http://dx.doi.org/10.1038/emm.2017.146 |
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author | Choi, Young Joon Kim, Sun Bean Kim, Jong Hoon Park, Su-Hyung Park, Moo Suk Kim, June Myung Han, Sang Hoon Shin, Eui-Cheol |
author_facet | Choi, Young Joon Kim, Sun Bean Kim, Jong Hoon Park, Su-Hyung Park, Moo Suk Kim, June Myung Han, Sang Hoon Shin, Eui-Cheol |
author_sort | Choi, Young Joon |
collection | PubMed |
description | Human cytomegalovirus (HCMV) establishes a lifelong chronic latent infection and often reactivates in immunocompromised patients. In addition, HCMV reactivates in patients with sepsis or other critical illnesses, particularly in patients with poor prognoses. However, the immunological characteristics of sepsis patients with HCMV reactivation have not been elucidated. In the present study, we examined T-cell responses in severe sepsis patients with and without HCMV reactivation. First, HCMV pp65-specific T-cell functions were assessed by intracellular cytokine staining (ICS) for IFN-γ, TNF-α, and MIP-1β and by CD107a staining. We analyzed the ICS data for each function individually and found no difference between the patient groups. However, the relative frequency of polyfunctional CD8(+) T cells was significantly decreased in sepsis patients with HCMV reactivation. Next, we examined programmed cell death protein 1 (PD-1) expression. It was significantly increased in the CD8(+) T-cell population in severe sepsis patients with HCMV reactivation, indicating CD8(+) T-cell exhaustion. Interestingly, the frequency of PD-1(+) cells in the CD8(+) T-cell population was inversely correlated with the relative frequency of polyfunctional CD8(+) T cells. Herein, we demonstrate that HCMV reactivation in severe sepsis patients is associated with PD-1 expression and impaired polyfunctionality of CD8(+) T cells. |
format | Online Article Text |
id | pubmed-5628278 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-56282782017-10-17 Impaired polyfunctionality of CD8(+) T cells in severe sepsis patients with human cytomegalovirus reactivation Choi, Young Joon Kim, Sun Bean Kim, Jong Hoon Park, Su-Hyung Park, Moo Suk Kim, June Myung Han, Sang Hoon Shin, Eui-Cheol Exp Mol Med Original Article Human cytomegalovirus (HCMV) establishes a lifelong chronic latent infection and often reactivates in immunocompromised patients. In addition, HCMV reactivates in patients with sepsis or other critical illnesses, particularly in patients with poor prognoses. However, the immunological characteristics of sepsis patients with HCMV reactivation have not been elucidated. In the present study, we examined T-cell responses in severe sepsis patients with and without HCMV reactivation. First, HCMV pp65-specific T-cell functions were assessed by intracellular cytokine staining (ICS) for IFN-γ, TNF-α, and MIP-1β and by CD107a staining. We analyzed the ICS data for each function individually and found no difference between the patient groups. However, the relative frequency of polyfunctional CD8(+) T cells was significantly decreased in sepsis patients with HCMV reactivation. Next, we examined programmed cell death protein 1 (PD-1) expression. It was significantly increased in the CD8(+) T-cell population in severe sepsis patients with HCMV reactivation, indicating CD8(+) T-cell exhaustion. Interestingly, the frequency of PD-1(+) cells in the CD8(+) T-cell population was inversely correlated with the relative frequency of polyfunctional CD8(+) T cells. Herein, we demonstrate that HCMV reactivation in severe sepsis patients is associated with PD-1 expression and impaired polyfunctionality of CD8(+) T cells. Nature Publishing Group 2017-09 2017-09-29 /pmc/articles/PMC5628278/ /pubmed/28960213 http://dx.doi.org/10.1038/emm.2017.146 Text en Copyright © 2017 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/ |
spellingShingle | Original Article Choi, Young Joon Kim, Sun Bean Kim, Jong Hoon Park, Su-Hyung Park, Moo Suk Kim, June Myung Han, Sang Hoon Shin, Eui-Cheol Impaired polyfunctionality of CD8(+) T cells in severe sepsis patients with human cytomegalovirus reactivation |
title | Impaired polyfunctionality of CD8(+) T cells in severe sepsis patients with human cytomegalovirus reactivation |
title_full | Impaired polyfunctionality of CD8(+) T cells in severe sepsis patients with human cytomegalovirus reactivation |
title_fullStr | Impaired polyfunctionality of CD8(+) T cells in severe sepsis patients with human cytomegalovirus reactivation |
title_full_unstemmed | Impaired polyfunctionality of CD8(+) T cells in severe sepsis patients with human cytomegalovirus reactivation |
title_short | Impaired polyfunctionality of CD8(+) T cells in severe sepsis patients with human cytomegalovirus reactivation |
title_sort | impaired polyfunctionality of cd8(+) t cells in severe sepsis patients with human cytomegalovirus reactivation |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5628278/ https://www.ncbi.nlm.nih.gov/pubmed/28960213 http://dx.doi.org/10.1038/emm.2017.146 |
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