Cloning, Assembly, and Modification of the Primary Human Cytomegalovirus Isolate Toledo by Yeast-Based Transformation-Associated Recombination

Genetic engineering of cytomegalovirus (CMV) currently relies on generating a bacterial artificial chromosome (BAC) by introducing a bacterial origin of replication into the viral genome using in vivo recombination in virally infected tissue culture cells. However, this process is inefficient, resul...

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Detalles Bibliográficos
Autores principales: Vashee, Sanjay, Stockwell, Timothy B., Alperovich, Nina, Denisova, Evgeniya A., Gibson, Daniel G., Cady, Kyle C., Miller, Kristofer, Kannan, Krishna, Malouli, Daniel, Crawford, Lindsey B., Voorhies, Alexander A., Bruening, Eric, Caposio, Patrizia, Früh, Klaus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5628293/
https://www.ncbi.nlm.nih.gov/pubmed/28989973
http://dx.doi.org/10.1128/mSphereDirect.00331-17

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