Cargando…

Tripartite symbiosis of Sophora tomentosa, rhizobia and arbuscular mycorhizal fungi

Sophora tomentosa is a pantropical legume species with potential for recovery of areas degraded by salinization, and for stabilization of sand dunes. However, few studies on this species have been carried out, and none regarding its symbiotic relationship with beneficial soil microorganisms. Therefo...

Descripción completa

Detalles Bibliográficos
Autores principales: Toma, Maíra Akemi, Soares de Carvalho, Teotonio, Azarias Guimarães, Amanda, Martins da Costa, Elaine, Savana da Silva, Jacqueline, de Souza Moreira, Fatima Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5628302/
https://www.ncbi.nlm.nih.gov/pubmed/28756029
http://dx.doi.org/10.1016/j.bjm.2017.03.007
_version_ 1783268855135600640
author Toma, Maíra Akemi
Soares de Carvalho, Teotonio
Azarias Guimarães, Amanda
Martins da Costa, Elaine
Savana da Silva, Jacqueline
de Souza Moreira, Fatima Maria
author_facet Toma, Maíra Akemi
Soares de Carvalho, Teotonio
Azarias Guimarães, Amanda
Martins da Costa, Elaine
Savana da Silva, Jacqueline
de Souza Moreira, Fatima Maria
author_sort Toma, Maíra Akemi
collection PubMed
description Sophora tomentosa is a pantropical legume species with potential for recovery of areas degraded by salinization, and for stabilization of sand dunes. However, few studies on this species have been carried out, and none regarding its symbiotic relationship with beneficial soil microorganisms. Therefore, this study aimed to evaluate the diversity of nitrogen-fixing bacteria isolated from nodules of Sophora tomentosa, and to analyze the occurrence of colonization of arbuscular mycorrhizal fungi on the roots of this legume in seafront soil. Thus, seeds, root nodules, and soil from the rhizosphere of Sophora tomentosa were collected. From the soil samples, trap cultures with this species were established to extract spores and to evaluate arbuscular mycorhizal fungi colonization in legume roots, as well as to capture rhizobia. Rhizobia strains were isolated from nodules collected in the field or from the trap cultures. Representative isolates of the groups obtained in the similarity dendrogram, based on phenotypic characteristics, had their 16S rRNA genes sequenced. The legume species showed nodules with indeterminate growth, and reddish color, distributed throughout the root. Fifty-one strains of these nodules were isolated, of which 21 were classified in the genus Bacillus, Brevibacillus, Paenibacillus, Rhizobium and especially Sinorhizobium. Strains closely related to Sinorhizobium adhaerens were the predominant bacteria in nodules. The other genera found, with the exception of Rhizobium, are probably endophytic bacteria in the nodules. Arbuscular mycorrhizal fungi was observed colonizing the roots, but arbuscular mycorhizal fungi spores were not found in the trap cultures. Therefore Sophora tomentosa is associated with both arbuscular mycorhizal fungi and nodulating nitrogen-fixing bacteria.
format Online
Article
Text
id pubmed-5628302
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-56283022017-10-10 Tripartite symbiosis of Sophora tomentosa, rhizobia and arbuscular mycorhizal fungi Toma, Maíra Akemi Soares de Carvalho, Teotonio Azarias Guimarães, Amanda Martins da Costa, Elaine Savana da Silva, Jacqueline de Souza Moreira, Fatima Maria Braz J Microbiol Research Paper Sophora tomentosa is a pantropical legume species with potential for recovery of areas degraded by salinization, and for stabilization of sand dunes. However, few studies on this species have been carried out, and none regarding its symbiotic relationship with beneficial soil microorganisms. Therefore, this study aimed to evaluate the diversity of nitrogen-fixing bacteria isolated from nodules of Sophora tomentosa, and to analyze the occurrence of colonization of arbuscular mycorrhizal fungi on the roots of this legume in seafront soil. Thus, seeds, root nodules, and soil from the rhizosphere of Sophora tomentosa were collected. From the soil samples, trap cultures with this species were established to extract spores and to evaluate arbuscular mycorhizal fungi colonization in legume roots, as well as to capture rhizobia. Rhizobia strains were isolated from nodules collected in the field or from the trap cultures. Representative isolates of the groups obtained in the similarity dendrogram, based on phenotypic characteristics, had their 16S rRNA genes sequenced. The legume species showed nodules with indeterminate growth, and reddish color, distributed throughout the root. Fifty-one strains of these nodules were isolated, of which 21 were classified in the genus Bacillus, Brevibacillus, Paenibacillus, Rhizobium and especially Sinorhizobium. Strains closely related to Sinorhizobium adhaerens were the predominant bacteria in nodules. The other genera found, with the exception of Rhizobium, are probably endophytic bacteria in the nodules. Arbuscular mycorrhizal fungi was observed colonizing the roots, but arbuscular mycorhizal fungi spores were not found in the trap cultures. Therefore Sophora tomentosa is associated with both arbuscular mycorhizal fungi and nodulating nitrogen-fixing bacteria. Elsevier 2017-06-29 /pmc/articles/PMC5628302/ /pubmed/28756029 http://dx.doi.org/10.1016/j.bjm.2017.03.007 Text en © 2017 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Paper
Toma, Maíra Akemi
Soares de Carvalho, Teotonio
Azarias Guimarães, Amanda
Martins da Costa, Elaine
Savana da Silva, Jacqueline
de Souza Moreira, Fatima Maria
Tripartite symbiosis of Sophora tomentosa, rhizobia and arbuscular mycorhizal fungi
title Tripartite symbiosis of Sophora tomentosa, rhizobia and arbuscular mycorhizal fungi
title_full Tripartite symbiosis of Sophora tomentosa, rhizobia and arbuscular mycorhizal fungi
title_fullStr Tripartite symbiosis of Sophora tomentosa, rhizobia and arbuscular mycorhizal fungi
title_full_unstemmed Tripartite symbiosis of Sophora tomentosa, rhizobia and arbuscular mycorhizal fungi
title_short Tripartite symbiosis of Sophora tomentosa, rhizobia and arbuscular mycorhizal fungi
title_sort tripartite symbiosis of sophora tomentosa, rhizobia and arbuscular mycorhizal fungi
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5628302/
https://www.ncbi.nlm.nih.gov/pubmed/28756029
http://dx.doi.org/10.1016/j.bjm.2017.03.007
work_keys_str_mv AT tomamairaakemi tripartitesymbiosisofsophoratomentosarhizobiaandarbuscularmycorhizalfungi
AT soaresdecarvalhoteotonio tripartitesymbiosisofsophoratomentosarhizobiaandarbuscularmycorhizalfungi
AT azariasguimaraesamanda tripartitesymbiosisofsophoratomentosarhizobiaandarbuscularmycorhizalfungi
AT martinsdacostaelaine tripartitesymbiosisofsophoratomentosarhizobiaandarbuscularmycorhizalfungi
AT savanadasilvajacqueline tripartitesymbiosisofsophoratomentosarhizobiaandarbuscularmycorhizalfungi
AT desouzamoreirafatimamaria tripartitesymbiosisofsophoratomentosarhizobiaandarbuscularmycorhizalfungi