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Melanogenesis inhibition activity of floralginsenoside A from Panax ginseng berry

BACKGROUND: Panax ginseng is a traditional herb used for medicinal purposes in eastern Asia. P. ginseng contains various ginsenosides with pharmacological effects. In this study, floralginsenoside A (FGA), ginsenoside Rd (GRD), and ginsenoside Re (GRE) were purified from P. ginseng berry. METHODS: C...

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Autores principales: Lee, Dae Young, Lee, Jongsung, Jeong, Yong Tae, Byun, Geon Hee, Kim, Jin Hee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5628341/
https://www.ncbi.nlm.nih.gov/pubmed/29021710
http://dx.doi.org/10.1016/j.jgr.2017.03.005
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author Lee, Dae Young
Lee, Jongsung
Jeong, Yong Tae
Byun, Geon Hee
Kim, Jin Hee
author_facet Lee, Dae Young
Lee, Jongsung
Jeong, Yong Tae
Byun, Geon Hee
Kim, Jin Hee
author_sort Lee, Dae Young
collection PubMed
description BACKGROUND: Panax ginseng is a traditional herb used for medicinal purposes in eastern Asia. P. ginseng contains various ginsenosides with pharmacological effects. In this study, floralginsenoside A (FGA), ginsenoside Rd (GRD), and ginsenoside Re (GRE) were purified from P. ginseng berry. METHODS: Chemical structures of FGA, GRD, and GRE were determined based on spectroscopic methods, including fast atom bombardment mass spectroscopy, ID-nuclear magnetic resonance, and infrared spectroscopy. Inhibitory activities of these compounds on melanogenesis were studied by measuring the expression of protein and melanin content in the melan-a cell line. This inhibitory activity was confirmed by observing pigmentation and tyrosinase activities of zebrafish. RESULTS: GRD, GRE, and FGA were not cytotoxic at concentrations less than 20μM, 80μM, and 160μM in melan-a cells, respectively. GRD, GRE, and FGA inhibited melanin biosynthesis in melan-a cells by 15.2%, 22.9%, and 23.9% at 20μM, 80μM, and 160μM, respectively. FGA was observed to display the most potent inhibitory effect. In addition, FGA decreased microphthalmia-associated transcription factor protein expression in a dose-dependent manner. Moreover, FGA induced extracellular signal-regulated kinase phosphorylation level in melan-a cells. In addition, melanin pigment content and tyrosinase activity in zebrafish treated with FGA at160μM were reduced. CONCLUSION: FGA showed the most potent inhibition of melanogenesis in both in vitro and in vivo studies. This study suggests that FGA purified from P. ginseng may be an effective melanogenesis inhibitor.
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spelling pubmed-56283412017-10-11 Melanogenesis inhibition activity of floralginsenoside A from Panax ginseng berry Lee, Dae Young Lee, Jongsung Jeong, Yong Tae Byun, Geon Hee Kim, Jin Hee J Ginseng Res Short Review Article BACKGROUND: Panax ginseng is a traditional herb used for medicinal purposes in eastern Asia. P. ginseng contains various ginsenosides with pharmacological effects. In this study, floralginsenoside A (FGA), ginsenoside Rd (GRD), and ginsenoside Re (GRE) were purified from P. ginseng berry. METHODS: Chemical structures of FGA, GRD, and GRE were determined based on spectroscopic methods, including fast atom bombardment mass spectroscopy, ID-nuclear magnetic resonance, and infrared spectroscopy. Inhibitory activities of these compounds on melanogenesis were studied by measuring the expression of protein and melanin content in the melan-a cell line. This inhibitory activity was confirmed by observing pigmentation and tyrosinase activities of zebrafish. RESULTS: GRD, GRE, and FGA were not cytotoxic at concentrations less than 20μM, 80μM, and 160μM in melan-a cells, respectively. GRD, GRE, and FGA inhibited melanin biosynthesis in melan-a cells by 15.2%, 22.9%, and 23.9% at 20μM, 80μM, and 160μM, respectively. FGA was observed to display the most potent inhibitory effect. In addition, FGA decreased microphthalmia-associated transcription factor protein expression in a dose-dependent manner. Moreover, FGA induced extracellular signal-regulated kinase phosphorylation level in melan-a cells. In addition, melanin pigment content and tyrosinase activity in zebrafish treated with FGA at160μM were reduced. CONCLUSION: FGA showed the most potent inhibition of melanogenesis in both in vitro and in vivo studies. This study suggests that FGA purified from P. ginseng may be an effective melanogenesis inhibitor. Elsevier 2017-10 2017-03-22 /pmc/articles/PMC5628341/ /pubmed/29021710 http://dx.doi.org/10.1016/j.jgr.2017.03.005 Text en © 2017 The Korean Society of Ginseng, Published by Elsevier Korea LLC. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Short Review Article
Lee, Dae Young
Lee, Jongsung
Jeong, Yong Tae
Byun, Geon Hee
Kim, Jin Hee
Melanogenesis inhibition activity of floralginsenoside A from Panax ginseng berry
title Melanogenesis inhibition activity of floralginsenoside A from Panax ginseng berry
title_full Melanogenesis inhibition activity of floralginsenoside A from Panax ginseng berry
title_fullStr Melanogenesis inhibition activity of floralginsenoside A from Panax ginseng berry
title_full_unstemmed Melanogenesis inhibition activity of floralginsenoside A from Panax ginseng berry
title_short Melanogenesis inhibition activity of floralginsenoside A from Panax ginseng berry
title_sort melanogenesis inhibition activity of floralginsenoside a from panax ginseng berry
topic Short Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5628341/
https://www.ncbi.nlm.nih.gov/pubmed/29021710
http://dx.doi.org/10.1016/j.jgr.2017.03.005
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