Cargando…

Harnessing mtDNA variation to resolve ambiguity in ‘Redfish’ sold in Europe

Morphology-based identification of North Atlantic Sebastes has long been controversial and misidentification may produce misleading data, with cascading consequences that negatively affect fisheries management and seafood labelling. North Atlantic Sebastes comprises of four species, commonly known a...

Descripción completa

Detalles Bibliográficos
Autores principales: Shum, Peter, Moore, Lauren, Pampoulie, Christophe, Di Muri, Cristina, Vandamme, Sara, Mariani, Stefano
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5628605/
https://www.ncbi.nlm.nih.gov/pubmed/29018597
http://dx.doi.org/10.7717/peerj.3746
_version_ 1783268918429745152
author Shum, Peter
Moore, Lauren
Pampoulie, Christophe
Di Muri, Cristina
Vandamme, Sara
Mariani, Stefano
author_facet Shum, Peter
Moore, Lauren
Pampoulie, Christophe
Di Muri, Cristina
Vandamme, Sara
Mariani, Stefano
author_sort Shum, Peter
collection PubMed
description Morphology-based identification of North Atlantic Sebastes has long been controversial and misidentification may produce misleading data, with cascading consequences that negatively affect fisheries management and seafood labelling. North Atlantic Sebastes comprises of four species, commonly known as ‘redfish’, but little is known about the number, identity and labelling accuracy of redfish species sold across Europe. We used a molecular approach to identify redfish species from ‘blind’ specimens to evaluate the performance of the Barcode of Life (BOLD) and Genbank databases, as well as carrying out a market product accuracy survey from retailers across Europe. The conventional BOLD approach proved ambiguous, and phylogenetic analysis based on mtDNA control region sequences provided a higher resolution for species identification. By sampling market products from four countries, we found the presence of two species of redfish (S. norvegicus and S. mentella) and one unidentified Pacific rockfish marketed in Europe. Furthermore, public databases revealed the existence of inaccurate reference sequences, likely stemming from species misidentification from previous studies, which currently hinders the efficacy of DNA methods for the identification of Sebastes market samples.
format Online
Article
Text
id pubmed-5628605
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher PeerJ Inc.
record_format MEDLINE/PubMed
spelling pubmed-56286052017-10-10 Harnessing mtDNA variation to resolve ambiguity in ‘Redfish’ sold in Europe Shum, Peter Moore, Lauren Pampoulie, Christophe Di Muri, Cristina Vandamme, Sara Mariani, Stefano PeerJ Aquaculture, Fisheries and Fish Science Morphology-based identification of North Atlantic Sebastes has long been controversial and misidentification may produce misleading data, with cascading consequences that negatively affect fisheries management and seafood labelling. North Atlantic Sebastes comprises of four species, commonly known as ‘redfish’, but little is known about the number, identity and labelling accuracy of redfish species sold across Europe. We used a molecular approach to identify redfish species from ‘blind’ specimens to evaluate the performance of the Barcode of Life (BOLD) and Genbank databases, as well as carrying out a market product accuracy survey from retailers across Europe. The conventional BOLD approach proved ambiguous, and phylogenetic analysis based on mtDNA control region sequences provided a higher resolution for species identification. By sampling market products from four countries, we found the presence of two species of redfish (S. norvegicus and S. mentella) and one unidentified Pacific rockfish marketed in Europe. Furthermore, public databases revealed the existence of inaccurate reference sequences, likely stemming from species misidentification from previous studies, which currently hinders the efficacy of DNA methods for the identification of Sebastes market samples. PeerJ Inc. 2017-10-02 /pmc/articles/PMC5628605/ /pubmed/29018597 http://dx.doi.org/10.7717/peerj.3746 Text en ©2017 Shum et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Aquaculture, Fisheries and Fish Science
Shum, Peter
Moore, Lauren
Pampoulie, Christophe
Di Muri, Cristina
Vandamme, Sara
Mariani, Stefano
Harnessing mtDNA variation to resolve ambiguity in ‘Redfish’ sold in Europe
title Harnessing mtDNA variation to resolve ambiguity in ‘Redfish’ sold in Europe
title_full Harnessing mtDNA variation to resolve ambiguity in ‘Redfish’ sold in Europe
title_fullStr Harnessing mtDNA variation to resolve ambiguity in ‘Redfish’ sold in Europe
title_full_unstemmed Harnessing mtDNA variation to resolve ambiguity in ‘Redfish’ sold in Europe
title_short Harnessing mtDNA variation to resolve ambiguity in ‘Redfish’ sold in Europe
title_sort harnessing mtdna variation to resolve ambiguity in ‘redfish’ sold in europe
topic Aquaculture, Fisheries and Fish Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5628605/
https://www.ncbi.nlm.nih.gov/pubmed/29018597
http://dx.doi.org/10.7717/peerj.3746
work_keys_str_mv AT shumpeter harnessingmtdnavariationtoresolveambiguityinredfishsoldineurope
AT moorelauren harnessingmtdnavariationtoresolveambiguityinredfishsoldineurope
AT pampouliechristophe harnessingmtdnavariationtoresolveambiguityinredfishsoldineurope
AT dimuricristina harnessingmtdnavariationtoresolveambiguityinredfishsoldineurope
AT vandammesara harnessingmtdnavariationtoresolveambiguityinredfishsoldineurope
AT marianistefano harnessingmtdnavariationtoresolveambiguityinredfishsoldineurope