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Harnessing mtDNA variation to resolve ambiguity in ‘Redfish’ sold in Europe
Morphology-based identification of North Atlantic Sebastes has long been controversial and misidentification may produce misleading data, with cascading consequences that negatively affect fisheries management and seafood labelling. North Atlantic Sebastes comprises of four species, commonly known a...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PeerJ Inc.
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5628605/ https://www.ncbi.nlm.nih.gov/pubmed/29018597 http://dx.doi.org/10.7717/peerj.3746 |
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author | Shum, Peter Moore, Lauren Pampoulie, Christophe Di Muri, Cristina Vandamme, Sara Mariani, Stefano |
author_facet | Shum, Peter Moore, Lauren Pampoulie, Christophe Di Muri, Cristina Vandamme, Sara Mariani, Stefano |
author_sort | Shum, Peter |
collection | PubMed |
description | Morphology-based identification of North Atlantic Sebastes has long been controversial and misidentification may produce misleading data, with cascading consequences that negatively affect fisheries management and seafood labelling. North Atlantic Sebastes comprises of four species, commonly known as ‘redfish’, but little is known about the number, identity and labelling accuracy of redfish species sold across Europe. We used a molecular approach to identify redfish species from ‘blind’ specimens to evaluate the performance of the Barcode of Life (BOLD) and Genbank databases, as well as carrying out a market product accuracy survey from retailers across Europe. The conventional BOLD approach proved ambiguous, and phylogenetic analysis based on mtDNA control region sequences provided a higher resolution for species identification. By sampling market products from four countries, we found the presence of two species of redfish (S. norvegicus and S. mentella) and one unidentified Pacific rockfish marketed in Europe. Furthermore, public databases revealed the existence of inaccurate reference sequences, likely stemming from species misidentification from previous studies, which currently hinders the efficacy of DNA methods for the identification of Sebastes market samples. |
format | Online Article Text |
id | pubmed-5628605 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | PeerJ Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-56286052017-10-10 Harnessing mtDNA variation to resolve ambiguity in ‘Redfish’ sold in Europe Shum, Peter Moore, Lauren Pampoulie, Christophe Di Muri, Cristina Vandamme, Sara Mariani, Stefano PeerJ Aquaculture, Fisheries and Fish Science Morphology-based identification of North Atlantic Sebastes has long been controversial and misidentification may produce misleading data, with cascading consequences that negatively affect fisheries management and seafood labelling. North Atlantic Sebastes comprises of four species, commonly known as ‘redfish’, but little is known about the number, identity and labelling accuracy of redfish species sold across Europe. We used a molecular approach to identify redfish species from ‘blind’ specimens to evaluate the performance of the Barcode of Life (BOLD) and Genbank databases, as well as carrying out a market product accuracy survey from retailers across Europe. The conventional BOLD approach proved ambiguous, and phylogenetic analysis based on mtDNA control region sequences provided a higher resolution for species identification. By sampling market products from four countries, we found the presence of two species of redfish (S. norvegicus and S. mentella) and one unidentified Pacific rockfish marketed in Europe. Furthermore, public databases revealed the existence of inaccurate reference sequences, likely stemming from species misidentification from previous studies, which currently hinders the efficacy of DNA methods for the identification of Sebastes market samples. PeerJ Inc. 2017-10-02 /pmc/articles/PMC5628605/ /pubmed/29018597 http://dx.doi.org/10.7717/peerj.3746 Text en ©2017 Shum et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
spellingShingle | Aquaculture, Fisheries and Fish Science Shum, Peter Moore, Lauren Pampoulie, Christophe Di Muri, Cristina Vandamme, Sara Mariani, Stefano Harnessing mtDNA variation to resolve ambiguity in ‘Redfish’ sold in Europe |
title | Harnessing mtDNA variation to resolve ambiguity in ‘Redfish’ sold in Europe |
title_full | Harnessing mtDNA variation to resolve ambiguity in ‘Redfish’ sold in Europe |
title_fullStr | Harnessing mtDNA variation to resolve ambiguity in ‘Redfish’ sold in Europe |
title_full_unstemmed | Harnessing mtDNA variation to resolve ambiguity in ‘Redfish’ sold in Europe |
title_short | Harnessing mtDNA variation to resolve ambiguity in ‘Redfish’ sold in Europe |
title_sort | harnessing mtdna variation to resolve ambiguity in ‘redfish’ sold in europe |
topic | Aquaculture, Fisheries and Fish Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5628605/ https://www.ncbi.nlm.nih.gov/pubmed/29018597 http://dx.doi.org/10.7717/peerj.3746 |
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