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Enhancing monellin production by Pichia pastoris at low cell induction concentration via effectively regulating methanol metabolism patterns and energy utilization efficiency
In heterologous protein productions by P. pastoris, methanol induction is generally initiated when cell concentration reaches very high density. The alternative strategy by initiating methanol induction at lower cells concentration was also reported to be effective in easing DO control, reducing tox...
Autores principales: | Jia, Luqiang, Tu, Tingyong, Huai, Qiangqiang, Sun, Jiaowen, Chen, Shanshan, Li, Xin, Shi, Zhongping, Ding, Jian |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5628809/ https://www.ncbi.nlm.nih.gov/pubmed/28981536 http://dx.doi.org/10.1371/journal.pone.0184602 |
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