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The effect of cavernous nerve traction on erectile function in rats

We performed this study to evaluate the effect of cavernous nerve (CN) traction on erectile function in rats. Thirty-two 8- week-old Sprague–Dawley rats were divided into four groups: control, 1-minute CN traction, 2-minute CN traction, and 2-minute CN crush. CN traction was performed using a glass...

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Autores principales: Li, Hao, Chen, Liping, Wang, Tao, Wang, Shaogang, Liu, Jihong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5628916/
https://www.ncbi.nlm.nih.gov/pubmed/28982169
http://dx.doi.org/10.1371/journal.pone.0186077
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author Li, Hao
Chen, Liping
Wang, Tao
Wang, Shaogang
Liu, Jihong
author_facet Li, Hao
Chen, Liping
Wang, Tao
Wang, Shaogang
Liu, Jihong
author_sort Li, Hao
collection PubMed
description We performed this study to evaluate the effect of cavernous nerve (CN) traction on erectile function in rats. Thirty-two 8- week-old Sprague–Dawley rats were divided into four groups: control, 1-minute CN traction, 2-minute CN traction, and 2-minute CN crush. CN traction was performed using a glass hook with a tensile force of 0.2 Newton. One month later, the mean arterial pressure (MAP) and intracavernosal pressure (ICP) in response to CN stimulation were measured to assess erectile function. The penis and major pelvic ganglion (MPG) were harvested to explore the expression of neuronal nitric oxide synthase (nNOS) and neurofilament, fibrosis and apoptosis. The ICP/MAP ratio was reduced in the 2-minute CN traction group compared with the control group (P < 0.05). The ICP/MAP ratio in the CN crush group was lower than in the other three groups (P < 0.05, for each). Expression of nNOS in both MPG and dorsal penile nerve was lower in the CN traction group than in the control group, but was higher than in the CN crush group (P < 0.05). Nerve fiber number in the dorsal penile nerve was reduced by 2-minute CN traction (P < 0.05). The ratios of collagen to smooth muscle content and the apoptosis were both increased the in 2-minute CN traction group compared with the control group (P < 0.05). The findings indicate that CN traction is an effective CN injury model and the injury it caused is relatively mild compared with the CN crush model.
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spelling pubmed-56289162017-10-20 The effect of cavernous nerve traction on erectile function in rats Li, Hao Chen, Liping Wang, Tao Wang, Shaogang Liu, Jihong PLoS One Research Article We performed this study to evaluate the effect of cavernous nerve (CN) traction on erectile function in rats. Thirty-two 8- week-old Sprague–Dawley rats were divided into four groups: control, 1-minute CN traction, 2-minute CN traction, and 2-minute CN crush. CN traction was performed using a glass hook with a tensile force of 0.2 Newton. One month later, the mean arterial pressure (MAP) and intracavernosal pressure (ICP) in response to CN stimulation were measured to assess erectile function. The penis and major pelvic ganglion (MPG) were harvested to explore the expression of neuronal nitric oxide synthase (nNOS) and neurofilament, fibrosis and apoptosis. The ICP/MAP ratio was reduced in the 2-minute CN traction group compared with the control group (P < 0.05). The ICP/MAP ratio in the CN crush group was lower than in the other three groups (P < 0.05, for each). Expression of nNOS in both MPG and dorsal penile nerve was lower in the CN traction group than in the control group, but was higher than in the CN crush group (P < 0.05). Nerve fiber number in the dorsal penile nerve was reduced by 2-minute CN traction (P < 0.05). The ratios of collagen to smooth muscle content and the apoptosis were both increased the in 2-minute CN traction group compared with the control group (P < 0.05). The findings indicate that CN traction is an effective CN injury model and the injury it caused is relatively mild compared with the CN crush model. Public Library of Science 2017-10-05 /pmc/articles/PMC5628916/ /pubmed/28982169 http://dx.doi.org/10.1371/journal.pone.0186077 Text en © 2017 Li et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Li, Hao
Chen, Liping
Wang, Tao
Wang, Shaogang
Liu, Jihong
The effect of cavernous nerve traction on erectile function in rats
title The effect of cavernous nerve traction on erectile function in rats
title_full The effect of cavernous nerve traction on erectile function in rats
title_fullStr The effect of cavernous nerve traction on erectile function in rats
title_full_unstemmed The effect of cavernous nerve traction on erectile function in rats
title_short The effect of cavernous nerve traction on erectile function in rats
title_sort effect of cavernous nerve traction on erectile function in rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5628916/
https://www.ncbi.nlm.nih.gov/pubmed/28982169
http://dx.doi.org/10.1371/journal.pone.0186077
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