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Experimental human-like model to assess the part of viable Legionella reaching the thoracic region after nebulization

The incidence of Legionnaires’ disease (LD) in European countries and the USA has been constantly increasing since 1998. Infection of humans occurs through aerosol inhalation. To bridge the existing gap between the concentration of Legionella in a water network and the deposition of bacteria within...

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Autores principales: Pourchez, Jérémie, Leclerc, Lara, Girardot, Françoise, Riffard, Serge, Prevot, Nathalie, Allegra, Séverine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5628919/
https://www.ncbi.nlm.nih.gov/pubmed/28982141
http://dx.doi.org/10.1371/journal.pone.0186042
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author Pourchez, Jérémie
Leclerc, Lara
Girardot, Françoise
Riffard, Serge
Prevot, Nathalie
Allegra, Séverine
author_facet Pourchez, Jérémie
Leclerc, Lara
Girardot, Françoise
Riffard, Serge
Prevot, Nathalie
Allegra, Séverine
author_sort Pourchez, Jérémie
collection PubMed
description The incidence of Legionnaires’ disease (LD) in European countries and the USA has been constantly increasing since 1998. Infection of humans occurs through aerosol inhalation. To bridge the existing gap between the concentration of Legionella in a water network and the deposition of bacteria within the thoracic region (assessment of the number of viable Legionella), we validated a model mimicking realistic exposure through the use of (i) recent technology for aerosol generation and (ii) a 3D replicate of the human upper respiratory tract. The model’s sensitivity was determined by monitoring the deposition of (i) aerosolized water and Tc(99m) radio-aerosol as controls, and (ii) bioaerosols generated from both Escherichia coli and Legionella pneumophila sg 1 suspensions. The numbers of viable Legionella prior to and after nebulization were provided by culture, flow cytometry and qPCR. This study was designed to obtain more realistic data on aerosol inhalation (vs. animal experimentation) and deposition at the thoracic region in the context of LD. Upon nebulization, 40% and 48% of the initial Legionella inoculum was made of cultivable and non-cultivable cells, respectively; 0.7% of both populations reached the filter holder mimicking the thoracic region in this setup. These results are in agreement with experimental data based on quantitative microbial risk assessment methods and bring new methods that may be useful for preventing LD.
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spelling pubmed-56289192017-10-20 Experimental human-like model to assess the part of viable Legionella reaching the thoracic region after nebulization Pourchez, Jérémie Leclerc, Lara Girardot, Françoise Riffard, Serge Prevot, Nathalie Allegra, Séverine PLoS One Research Article The incidence of Legionnaires’ disease (LD) in European countries and the USA has been constantly increasing since 1998. Infection of humans occurs through aerosol inhalation. To bridge the existing gap between the concentration of Legionella in a water network and the deposition of bacteria within the thoracic region (assessment of the number of viable Legionella), we validated a model mimicking realistic exposure through the use of (i) recent technology for aerosol generation and (ii) a 3D replicate of the human upper respiratory tract. The model’s sensitivity was determined by monitoring the deposition of (i) aerosolized water and Tc(99m) radio-aerosol as controls, and (ii) bioaerosols generated from both Escherichia coli and Legionella pneumophila sg 1 suspensions. The numbers of viable Legionella prior to and after nebulization were provided by culture, flow cytometry and qPCR. This study was designed to obtain more realistic data on aerosol inhalation (vs. animal experimentation) and deposition at the thoracic region in the context of LD. Upon nebulization, 40% and 48% of the initial Legionella inoculum was made of cultivable and non-cultivable cells, respectively; 0.7% of both populations reached the filter holder mimicking the thoracic region in this setup. These results are in agreement with experimental data based on quantitative microbial risk assessment methods and bring new methods that may be useful for preventing LD. Public Library of Science 2017-10-05 /pmc/articles/PMC5628919/ /pubmed/28982141 http://dx.doi.org/10.1371/journal.pone.0186042 Text en © 2017 Pourchez et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Pourchez, Jérémie
Leclerc, Lara
Girardot, Françoise
Riffard, Serge
Prevot, Nathalie
Allegra, Séverine
Experimental human-like model to assess the part of viable Legionella reaching the thoracic region after nebulization
title Experimental human-like model to assess the part of viable Legionella reaching the thoracic region after nebulization
title_full Experimental human-like model to assess the part of viable Legionella reaching the thoracic region after nebulization
title_fullStr Experimental human-like model to assess the part of viable Legionella reaching the thoracic region after nebulization
title_full_unstemmed Experimental human-like model to assess the part of viable Legionella reaching the thoracic region after nebulization
title_short Experimental human-like model to assess the part of viable Legionella reaching the thoracic region after nebulization
title_sort experimental human-like model to assess the part of viable legionella reaching the thoracic region after nebulization
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5628919/
https://www.ncbi.nlm.nih.gov/pubmed/28982141
http://dx.doi.org/10.1371/journal.pone.0186042
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