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Natural and cross-inducible anti-SIV antibodies in Mauritian cynomolgus macaques

Cynomolgus macaques are an increasingly important nonhuman primate model for HIV vaccine research. SIV-free animals without pre-existing anti-SIV immune responses are generally needed to evaluate the effect of vaccine-induced immune responses against the vaccine epitopes. Here, in order to select su...

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Autores principales: Li, Hongzhao, Nykoluk, Mikaela, Li, Lin, Liu, Lewis R., Omange, Robert W., Soule, Geoff, Schroeder, Lukas T., Toledo, Nikki, Kashem, Mohammad Abul, Correia-Pinto, Jorge F., Liang, Binhua, Schultz-Darken, Nancy, Alonso, Maria J., Whitney, James B., Plummer, Francis A., Luo, Ma
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5628977/
https://www.ncbi.nlm.nih.gov/pubmed/28982126
http://dx.doi.org/10.1371/journal.pone.0186079
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author Li, Hongzhao
Nykoluk, Mikaela
Li, Lin
Liu, Lewis R.
Omange, Robert W.
Soule, Geoff
Schroeder, Lukas T.
Toledo, Nikki
Kashem, Mohammad Abul
Correia-Pinto, Jorge F.
Liang, Binhua
Schultz-Darken, Nancy
Alonso, Maria J.
Whitney, James B.
Plummer, Francis A.
Luo, Ma
author_facet Li, Hongzhao
Nykoluk, Mikaela
Li, Lin
Liu, Lewis R.
Omange, Robert W.
Soule, Geoff
Schroeder, Lukas T.
Toledo, Nikki
Kashem, Mohammad Abul
Correia-Pinto, Jorge F.
Liang, Binhua
Schultz-Darken, Nancy
Alonso, Maria J.
Whitney, James B.
Plummer, Francis A.
Luo, Ma
author_sort Li, Hongzhao
collection PubMed
description Cynomolgus macaques are an increasingly important nonhuman primate model for HIV vaccine research. SIV-free animals without pre-existing anti-SIV immune responses are generally needed to evaluate the effect of vaccine-induced immune responses against the vaccine epitopes. Here, in order to select such animals for vaccine studies, we screened 108 naïve female Mauritian cynomolgus macaques for natural (baseline) antibodies to SIV antigens using a Bio-Plex multiplex system. The antigens included twelve 20mer peptides overlapping the twelve SIV protease cleavage sites (-10/+10), respectively (PCS peptides), and three non-PCS Gag or Env peptides. Natural antibodies to SIV antigens were detected in subsets of monkeys. The antibody reactivity to SIV was further confirmed by Western blot using purified recombinant SIV Gag and Env proteins. As expected, the immunization of monkeys with PCS antigens elicited anti-PCS antibodies. However, unexpectedly, antibodies to non-PCS peptides were also induced, as shown by both Bio-Plex and Western blot analyses, while the non-PCS peptides do not share sequence homology with PCS peptides. The presence of natural and vaccine cross-inducible SIV antibodies in Mauritian cynomolgus macaques should be considered in animal selection, experimental design and result interpretation, for their best use in HIV vaccine research.
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spelling pubmed-56289772017-10-20 Natural and cross-inducible anti-SIV antibodies in Mauritian cynomolgus macaques Li, Hongzhao Nykoluk, Mikaela Li, Lin Liu, Lewis R. Omange, Robert W. Soule, Geoff Schroeder, Lukas T. Toledo, Nikki Kashem, Mohammad Abul Correia-Pinto, Jorge F. Liang, Binhua Schultz-Darken, Nancy Alonso, Maria J. Whitney, James B. Plummer, Francis A. Luo, Ma PLoS One Research Article Cynomolgus macaques are an increasingly important nonhuman primate model for HIV vaccine research. SIV-free animals without pre-existing anti-SIV immune responses are generally needed to evaluate the effect of vaccine-induced immune responses against the vaccine epitopes. Here, in order to select such animals for vaccine studies, we screened 108 naïve female Mauritian cynomolgus macaques for natural (baseline) antibodies to SIV antigens using a Bio-Plex multiplex system. The antigens included twelve 20mer peptides overlapping the twelve SIV protease cleavage sites (-10/+10), respectively (PCS peptides), and three non-PCS Gag or Env peptides. Natural antibodies to SIV antigens were detected in subsets of monkeys. The antibody reactivity to SIV was further confirmed by Western blot using purified recombinant SIV Gag and Env proteins. As expected, the immunization of monkeys with PCS antigens elicited anti-PCS antibodies. However, unexpectedly, antibodies to non-PCS peptides were also induced, as shown by both Bio-Plex and Western blot analyses, while the non-PCS peptides do not share sequence homology with PCS peptides. The presence of natural and vaccine cross-inducible SIV antibodies in Mauritian cynomolgus macaques should be considered in animal selection, experimental design and result interpretation, for their best use in HIV vaccine research. Public Library of Science 2017-10-05 /pmc/articles/PMC5628977/ /pubmed/28982126 http://dx.doi.org/10.1371/journal.pone.0186079 Text en © 2017 Li et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Li, Hongzhao
Nykoluk, Mikaela
Li, Lin
Liu, Lewis R.
Omange, Robert W.
Soule, Geoff
Schroeder, Lukas T.
Toledo, Nikki
Kashem, Mohammad Abul
Correia-Pinto, Jorge F.
Liang, Binhua
Schultz-Darken, Nancy
Alonso, Maria J.
Whitney, James B.
Plummer, Francis A.
Luo, Ma
Natural and cross-inducible anti-SIV antibodies in Mauritian cynomolgus macaques
title Natural and cross-inducible anti-SIV antibodies in Mauritian cynomolgus macaques
title_full Natural and cross-inducible anti-SIV antibodies in Mauritian cynomolgus macaques
title_fullStr Natural and cross-inducible anti-SIV antibodies in Mauritian cynomolgus macaques
title_full_unstemmed Natural and cross-inducible anti-SIV antibodies in Mauritian cynomolgus macaques
title_short Natural and cross-inducible anti-SIV antibodies in Mauritian cynomolgus macaques
title_sort natural and cross-inducible anti-siv antibodies in mauritian cynomolgus macaques
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5628977/
https://www.ncbi.nlm.nih.gov/pubmed/28982126
http://dx.doi.org/10.1371/journal.pone.0186079
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