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The identification of sympatric cryptic free-living nematode species in the Antarctic intertidal

The diversity of free-living nematodes in the beaches of two Antarctic islands, King George and Deception islands was investigated. We used morphological and molecular (LSU, and two fragments of SSU sequences) approaches to evaluate 236 nematodes. Specimens were assigned to at least genera using mor...

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Autores principales: Lee, Matthew R., Canales-Aguirre, Cristian B., Nuñez, Daniela, Pérez, Karla, Hernández, Crisitan E., Brante, Antonio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5629031/
https://www.ncbi.nlm.nih.gov/pubmed/28982192
http://dx.doi.org/10.1371/journal.pone.0186140
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author Lee, Matthew R.
Canales-Aguirre, Cristian B.
Nuñez, Daniela
Pérez, Karla
Hernández, Crisitan E.
Brante, Antonio
author_facet Lee, Matthew R.
Canales-Aguirre, Cristian B.
Nuñez, Daniela
Pérez, Karla
Hernández, Crisitan E.
Brante, Antonio
author_sort Lee, Matthew R.
collection PubMed
description The diversity of free-living nematodes in the beaches of two Antarctic islands, King George and Deception islands was investigated. We used morphological and molecular (LSU, and two fragments of SSU sequences) approaches to evaluate 236 nematodes. Specimens were assigned to at least genera using morphology and were assessed for the presence of cryptic speciation. The following genera were identified: Halomonhystera, Litoditis, Enoploides, Chromadorita, Theristus, Oncholaimus, Viscosia, Gammanema, Bathylaimus, Choanolaimus, and Paracanthonchus; along with specimens from the families Anticomidae and Linhomoeidae. Cryptic speciation was identified within the genera Halomonhystera and Litoditis. All of the cryptic species identified live sympatrically. The two cryptic species of Halomonhystera exhibited no significant morphological differences. However, Litoditis species 2 was significantly larger than Litoditis species 1. The utility of molecular data in confirming the identifications of some of the morphologically more challenging families of nematodes was demonstrated. In terms of which molecular sequences to use for the identification of free-living nematodes, the SSU sequences were more variable than the LSU sequences, and thus provided more resolution in the identification of cryptic speciation. Finally, despite the considerable amount of time and effort required to put together genetic and morphological data, the resulting advance in our understanding of diversity and ecology of free-living marine nematodes, makes that effort worthwhile.
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spelling pubmed-56290312017-10-20 The identification of sympatric cryptic free-living nematode species in the Antarctic intertidal Lee, Matthew R. Canales-Aguirre, Cristian B. Nuñez, Daniela Pérez, Karla Hernández, Crisitan E. Brante, Antonio PLoS One Research Article The diversity of free-living nematodes in the beaches of two Antarctic islands, King George and Deception islands was investigated. We used morphological and molecular (LSU, and two fragments of SSU sequences) approaches to evaluate 236 nematodes. Specimens were assigned to at least genera using morphology and were assessed for the presence of cryptic speciation. The following genera were identified: Halomonhystera, Litoditis, Enoploides, Chromadorita, Theristus, Oncholaimus, Viscosia, Gammanema, Bathylaimus, Choanolaimus, and Paracanthonchus; along with specimens from the families Anticomidae and Linhomoeidae. Cryptic speciation was identified within the genera Halomonhystera and Litoditis. All of the cryptic species identified live sympatrically. The two cryptic species of Halomonhystera exhibited no significant morphological differences. However, Litoditis species 2 was significantly larger than Litoditis species 1. The utility of molecular data in confirming the identifications of some of the morphologically more challenging families of nematodes was demonstrated. In terms of which molecular sequences to use for the identification of free-living nematodes, the SSU sequences were more variable than the LSU sequences, and thus provided more resolution in the identification of cryptic speciation. Finally, despite the considerable amount of time and effort required to put together genetic and morphological data, the resulting advance in our understanding of diversity and ecology of free-living marine nematodes, makes that effort worthwhile. Public Library of Science 2017-10-05 /pmc/articles/PMC5629031/ /pubmed/28982192 http://dx.doi.org/10.1371/journal.pone.0186140 Text en © 2017 Lee et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Lee, Matthew R.
Canales-Aguirre, Cristian B.
Nuñez, Daniela
Pérez, Karla
Hernández, Crisitan E.
Brante, Antonio
The identification of sympatric cryptic free-living nematode species in the Antarctic intertidal
title The identification of sympatric cryptic free-living nematode species in the Antarctic intertidal
title_full The identification of sympatric cryptic free-living nematode species in the Antarctic intertidal
title_fullStr The identification of sympatric cryptic free-living nematode species in the Antarctic intertidal
title_full_unstemmed The identification of sympatric cryptic free-living nematode species in the Antarctic intertidal
title_short The identification of sympatric cryptic free-living nematode species in the Antarctic intertidal
title_sort identification of sympatric cryptic free-living nematode species in the antarctic intertidal
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5629031/
https://www.ncbi.nlm.nih.gov/pubmed/28982192
http://dx.doi.org/10.1371/journal.pone.0186140
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