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Solid-phase reverse transfection for intracellular delivery of functionally active proteins

Delivery of large and functionally active biomolecules across cell membranes presents a challenge in cell biological experimentation. For this purpose, we developed a novel solid-phase reverse transfection method that is suitable for the intracellular delivery of proteins into mammalian cells with p...

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Detalles Bibliográficos
Autores principales: Bulkescher, Ruben, Starkuviene, Vytaute, Erfle, Holger
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5630038/
https://www.ncbi.nlm.nih.gov/pubmed/28874398
http://dx.doi.org/10.1101/gr.215103.116
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author Bulkescher, Ruben
Starkuviene, Vytaute
Erfle, Holger
author_facet Bulkescher, Ruben
Starkuviene, Vytaute
Erfle, Holger
author_sort Bulkescher, Ruben
collection PubMed
description Delivery of large and functionally active biomolecules across cell membranes presents a challenge in cell biological experimentation. For this purpose, we developed a novel solid-phase reverse transfection method that is suitable for the intracellular delivery of proteins into mammalian cells with preservation of their function. We show results for diverse application areas of the method, ranging from antibody-mediated inhibition of protein function to CRISPR/Cas9-based gene editing in living cells. Our method enables prefabrication of “ready to transfect” substrates carrying diverse proteins. This allows their easy distribution and standardization of biological assays across different laboratories.
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spelling pubmed-56300382018-04-01 Solid-phase reverse transfection for intracellular delivery of functionally active proteins Bulkescher, Ruben Starkuviene, Vytaute Erfle, Holger Genome Res Method Delivery of large and functionally active biomolecules across cell membranes presents a challenge in cell biological experimentation. For this purpose, we developed a novel solid-phase reverse transfection method that is suitable for the intracellular delivery of proteins into mammalian cells with preservation of their function. We show results for diverse application areas of the method, ranging from antibody-mediated inhibition of protein function to CRISPR/Cas9-based gene editing in living cells. Our method enables prefabrication of “ready to transfect” substrates carrying diverse proteins. This allows their easy distribution and standardization of biological assays across different laboratories. Cold Spring Harbor Laboratory Press 2017-10 /pmc/articles/PMC5630038/ /pubmed/28874398 http://dx.doi.org/10.1101/gr.215103.116 Text en © 2017 Bulkescher et al.; Published by Cold Spring Harbor Laboratory Press http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genome.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.
spellingShingle Method
Bulkescher, Ruben
Starkuviene, Vytaute
Erfle, Holger
Solid-phase reverse transfection for intracellular delivery of functionally active proteins
title Solid-phase reverse transfection for intracellular delivery of functionally active proteins
title_full Solid-phase reverse transfection for intracellular delivery of functionally active proteins
title_fullStr Solid-phase reverse transfection for intracellular delivery of functionally active proteins
title_full_unstemmed Solid-phase reverse transfection for intracellular delivery of functionally active proteins
title_short Solid-phase reverse transfection for intracellular delivery of functionally active proteins
title_sort solid-phase reverse transfection for intracellular delivery of functionally active proteins
topic Method
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5630038/
https://www.ncbi.nlm.nih.gov/pubmed/28874398
http://dx.doi.org/10.1101/gr.215103.116
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