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Efficient intracellular delivery makes cancer cells sensitive to nanoemulsive chemodrugs

Evodiamine has been documented to possess activities in numerous cancer cells. Our preliminary study showed that A549 cells were insensitive to evodiamine. In this paper, A549 cells are sensitive to nanoemulsive evodiamine (EVONE) through an efficient intracellular and systematic delivery. EVONE ent...

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Detalles Bibliográficos
Autores principales: Liu, Shan, Chen, Dilong, Yuan, Yuming, Zhang, Xue, Li, Yao, Yan, Shenglei, Zhang, Jingqing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5630310/
https://www.ncbi.nlm.nih.gov/pubmed/29029410
http://dx.doi.org/10.18632/oncotarget.17536
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author Liu, Shan
Chen, Dilong
Yuan, Yuming
Zhang, Xue
Li, Yao
Yan, Shenglei
Zhang, Jingqing
author_facet Liu, Shan
Chen, Dilong
Yuan, Yuming
Zhang, Xue
Li, Yao
Yan, Shenglei
Zhang, Jingqing
author_sort Liu, Shan
collection PubMed
description Evodiamine has been documented to possess activities in numerous cancer cells. Our preliminary study showed that A549 cells were insensitive to evodiamine. In this paper, A549 cells are sensitive to nanoemulsive evodiamine (EVONE) through an efficient intracellular and systematic delivery. EVONE entered tumor cells by energy-dependent and mainly through clathrin-mediated endocytosis. EVONE exerted a higher cytotoxicity in a dose- and time-dependent manner. The enhanced induction of cell cycle arrest was ascribed to the down-regulation of cyclin B and cyclin dependent kinase 1, while the enhanced induction of apoptosis was due to the activation of caspase −3, −8 and −9 and the decreased B-cell lymphoma 2/ assaciated X protein ratio. Furthermore, the in vivo kinetic, bioavailability and in situ absorption characteristics of EVONE were much better than those of free evodiamine. The cancer cells insensitive to free chemodrugs became sensitive to nanoemulsive chemodrugs.
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spelling pubmed-56303102017-10-12 Efficient intracellular delivery makes cancer cells sensitive to nanoemulsive chemodrugs Liu, Shan Chen, Dilong Yuan, Yuming Zhang, Xue Li, Yao Yan, Shenglei Zhang, Jingqing Oncotarget Research Paper Evodiamine has been documented to possess activities in numerous cancer cells. Our preliminary study showed that A549 cells were insensitive to evodiamine. In this paper, A549 cells are sensitive to nanoemulsive evodiamine (EVONE) through an efficient intracellular and systematic delivery. EVONE entered tumor cells by energy-dependent and mainly through clathrin-mediated endocytosis. EVONE exerted a higher cytotoxicity in a dose- and time-dependent manner. The enhanced induction of cell cycle arrest was ascribed to the down-regulation of cyclin B and cyclin dependent kinase 1, while the enhanced induction of apoptosis was due to the activation of caspase −3, −8 and −9 and the decreased B-cell lymphoma 2/ assaciated X protein ratio. Furthermore, the in vivo kinetic, bioavailability and in situ absorption characteristics of EVONE were much better than those of free evodiamine. The cancer cells insensitive to free chemodrugs became sensitive to nanoemulsive chemodrugs. Impact Journals LLC 2017-04-28 /pmc/articles/PMC5630310/ /pubmed/29029410 http://dx.doi.org/10.18632/oncotarget.17536 Text en Copyright: © 2017 Liu et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Liu, Shan
Chen, Dilong
Yuan, Yuming
Zhang, Xue
Li, Yao
Yan, Shenglei
Zhang, Jingqing
Efficient intracellular delivery makes cancer cells sensitive to nanoemulsive chemodrugs
title Efficient intracellular delivery makes cancer cells sensitive to nanoemulsive chemodrugs
title_full Efficient intracellular delivery makes cancer cells sensitive to nanoemulsive chemodrugs
title_fullStr Efficient intracellular delivery makes cancer cells sensitive to nanoemulsive chemodrugs
title_full_unstemmed Efficient intracellular delivery makes cancer cells sensitive to nanoemulsive chemodrugs
title_short Efficient intracellular delivery makes cancer cells sensitive to nanoemulsive chemodrugs
title_sort efficient intracellular delivery makes cancer cells sensitive to nanoemulsive chemodrugs
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5630310/
https://www.ncbi.nlm.nih.gov/pubmed/29029410
http://dx.doi.org/10.18632/oncotarget.17536
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