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Comparative Genome Analysis of Fusobacterium nucleatum

Fusobacterium nucleatum is considered to be a key oral bacterium in recruiting periodontal pathogens into subgingival dental plaque. Currently F. nucleatum can be subdivided into five subspecies. Our previous genome analysis of F. nucleatum W1481 (referred to hereafter as W1481), isolated from an 8-...

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Autores principales: Ang, Mia Yang, Dutta, Avirup, Wee, Wei Yee, Dymock, David, Paterson, Ian C., Choo, Siew Woh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5630926/
https://www.ncbi.nlm.nih.gov/pubmed/27540086
http://dx.doi.org/10.1093/gbe/evw199
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author Ang, Mia Yang
Dutta, Avirup
Wee, Wei Yee
Dymock, David
Paterson, Ian C.
Choo, Siew Woh
author_facet Ang, Mia Yang
Dutta, Avirup
Wee, Wei Yee
Dymock, David
Paterson, Ian C.
Choo, Siew Woh
author_sort Ang, Mia Yang
collection PubMed
description Fusobacterium nucleatum is considered to be a key oral bacterium in recruiting periodontal pathogens into subgingival dental plaque. Currently F. nucleatum can be subdivided into five subspecies. Our previous genome analysis of F. nucleatum W1481 (referred to hereafter as W1481), isolated from an 8-mm periodontal pocket in a patient with chronic periodontitis, suggested the possibility of a new subspecies. To further investigate the biology and relationships of this possible subspecies with other known subspecies, we performed comparative analysis between W1481 and 35 genome sequences represented by the five known Fusobacterium subspecies. Our analyses suggest that W1481 is most likely a new F. nucleatum subspecies, supported by evidence from phylogenetic analyses and maximal unique match indices (MUMi). Interestingly, we found a horizontally transferred W1481-specific genomic island harboring the tripartite ATP-independent (TRAP)-like transporter genes, suggesting this bacterium might have a high-affinity transport system for the C4-dicarboxylates malate, succinate, and fumarate. Moreover, we found virulence genes in the W1481 genome that may provide a strong defense mechanism which might enable it to colonize and survive within the host by evading immune surveillance. This comparative study provides better understanding of F. nucleatum and the basis for future functional work on this important pathogen.
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spelling pubmed-56309262017-11-01 Comparative Genome Analysis of Fusobacterium nucleatum Ang, Mia Yang Dutta, Avirup Wee, Wei Yee Dymock, David Paterson, Ian C. Choo, Siew Woh Genome Biol Evol Research Article Fusobacterium nucleatum is considered to be a key oral bacterium in recruiting periodontal pathogens into subgingival dental plaque. Currently F. nucleatum can be subdivided into five subspecies. Our previous genome analysis of F. nucleatum W1481 (referred to hereafter as W1481), isolated from an 8-mm periodontal pocket in a patient with chronic periodontitis, suggested the possibility of a new subspecies. To further investigate the biology and relationships of this possible subspecies with other known subspecies, we performed comparative analysis between W1481 and 35 genome sequences represented by the five known Fusobacterium subspecies. Our analyses suggest that W1481 is most likely a new F. nucleatum subspecies, supported by evidence from phylogenetic analyses and maximal unique match indices (MUMi). Interestingly, we found a horizontally transferred W1481-specific genomic island harboring the tripartite ATP-independent (TRAP)-like transporter genes, suggesting this bacterium might have a high-affinity transport system for the C4-dicarboxylates malate, succinate, and fumarate. Moreover, we found virulence genes in the W1481 genome that may provide a strong defense mechanism which might enable it to colonize and survive within the host by evading immune surveillance. This comparative study provides better understanding of F. nucleatum and the basis for future functional work on this important pathogen. Oxford University Press 2016-08-18 /pmc/articles/PMC5630926/ /pubmed/27540086 http://dx.doi.org/10.1093/gbe/evw199 Text en © The Author 2016. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Research Article
Ang, Mia Yang
Dutta, Avirup
Wee, Wei Yee
Dymock, David
Paterson, Ian C.
Choo, Siew Woh
Comparative Genome Analysis of Fusobacterium nucleatum
title Comparative Genome Analysis of Fusobacterium nucleatum
title_full Comparative Genome Analysis of Fusobacterium nucleatum
title_fullStr Comparative Genome Analysis of Fusobacterium nucleatum
title_full_unstemmed Comparative Genome Analysis of Fusobacterium nucleatum
title_short Comparative Genome Analysis of Fusobacterium nucleatum
title_sort comparative genome analysis of fusobacterium nucleatum
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5630926/
https://www.ncbi.nlm.nih.gov/pubmed/27540086
http://dx.doi.org/10.1093/gbe/evw199
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