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Bone resorptive activity in symptomatic and asymptomatic apical lesions of endodontic origin

OBJECTIVES: The aim of this study is to assess the levels and diagnostic accuracy of a set of bone resorption biomarkers, including TRAP-5, RANKL, and OPG in symptomatic and asymptomatic apical lesions and controls. MATERIALS AND METHODS: Apical tissues from symptomatic and asymptomatic apical perio...

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Autores principales: Salinas-Muñoz, M., Garrido-Flores, M., Baeza, M., Huamán-Chipana, P., García-Sesnich, J., Bologna, R., Vernal, R., Hernández, M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5632344/
https://www.ncbi.nlm.nih.gov/pubmed/28261746
http://dx.doi.org/10.1007/s00784-017-2062-x
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author Salinas-Muñoz, M.
Garrido-Flores, M.
Baeza, M.
Huamán-Chipana, P.
García-Sesnich, J.
Bologna, R.
Vernal, R.
Hernández, M.
author_facet Salinas-Muñoz, M.
Garrido-Flores, M.
Baeza, M.
Huamán-Chipana, P.
García-Sesnich, J.
Bologna, R.
Vernal, R.
Hernández, M.
author_sort Salinas-Muñoz, M.
collection PubMed
description OBJECTIVES: The aim of this study is to assess the levels and diagnostic accuracy of a set of bone resorption biomarkers, including TRAP-5, RANKL, and OPG in symptomatic and asymptomatic apical lesions and controls. MATERIALS AND METHODS: Apical tissues from symptomatic and asymptomatic apical periodontitis patients and periodontal ligaments from healthy teeth extracted for orthodontic reasons were processed for tissue homogenization and the levels of TRAP-5, RANKL, and OPG were determined by multiplex assay. Marker levels were analyzed by Kruskal Wallis test, and diagnostic accuracy was analyzed with ROC curves. RESULTS: Higher levels of RANKL, OPG, and RANKL/OPG ratio were determined in both types of apical lesions compared to healthy periodontal ligament, whereas higher TRAP-5 levels were found only in symptomatic apical lesions (p < 0.05). OPG, RANKL, and RANKL/OPG ratio showed diagnostic potential to identify apical lesions versus healthy controls (AUC = 0.69, p < 0.05); while TRAP-5 showed a potential to discriminate symptomatic versus asymptomatic apical periodontitis (AUC = 0.71, p < 0.05) and healthy controls (AUC = 0.83, p < 0.05). CONCLUSIONS: Apical lesions showed higher RANKL and OPG levels than healthy tissues. TRAP-5 levels were the highest in symptomatic apical lesions, suggesting that these represent a progressive state, and showed diagnostic potential. CLINICAL RELEVANCE: Clinically symptomatic apical periodontitis might represent biologically progressive apical lesions based on TRAP5 levels. TRAP5 has diagnostic potential to identify these lesions, representing a candidate prognostic biomarker.
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spelling pubmed-56323442017-10-23 Bone resorptive activity in symptomatic and asymptomatic apical lesions of endodontic origin Salinas-Muñoz, M. Garrido-Flores, M. Baeza, M. Huamán-Chipana, P. García-Sesnich, J. Bologna, R. Vernal, R. Hernández, M. Clin Oral Investig Original Article OBJECTIVES: The aim of this study is to assess the levels and diagnostic accuracy of a set of bone resorption biomarkers, including TRAP-5, RANKL, and OPG in symptomatic and asymptomatic apical lesions and controls. MATERIALS AND METHODS: Apical tissues from symptomatic and asymptomatic apical periodontitis patients and periodontal ligaments from healthy teeth extracted for orthodontic reasons were processed for tissue homogenization and the levels of TRAP-5, RANKL, and OPG were determined by multiplex assay. Marker levels were analyzed by Kruskal Wallis test, and diagnostic accuracy was analyzed with ROC curves. RESULTS: Higher levels of RANKL, OPG, and RANKL/OPG ratio were determined in both types of apical lesions compared to healthy periodontal ligament, whereas higher TRAP-5 levels were found only in symptomatic apical lesions (p < 0.05). OPG, RANKL, and RANKL/OPG ratio showed diagnostic potential to identify apical lesions versus healthy controls (AUC = 0.69, p < 0.05); while TRAP-5 showed a potential to discriminate symptomatic versus asymptomatic apical periodontitis (AUC = 0.71, p < 0.05) and healthy controls (AUC = 0.83, p < 0.05). CONCLUSIONS: Apical lesions showed higher RANKL and OPG levels than healthy tissues. TRAP-5 levels were the highest in symptomatic apical lesions, suggesting that these represent a progressive state, and showed diagnostic potential. CLINICAL RELEVANCE: Clinically symptomatic apical periodontitis might represent biologically progressive apical lesions based on TRAP5 levels. TRAP5 has diagnostic potential to identify these lesions, representing a candidate prognostic biomarker. Springer Berlin Heidelberg 2017-03-06 2017 /pmc/articles/PMC5632344/ /pubmed/28261746 http://dx.doi.org/10.1007/s00784-017-2062-x Text en © The Author(s) 2017 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Salinas-Muñoz, M.
Garrido-Flores, M.
Baeza, M.
Huamán-Chipana, P.
García-Sesnich, J.
Bologna, R.
Vernal, R.
Hernández, M.
Bone resorptive activity in symptomatic and asymptomatic apical lesions of endodontic origin
title Bone resorptive activity in symptomatic and asymptomatic apical lesions of endodontic origin
title_full Bone resorptive activity in symptomatic and asymptomatic apical lesions of endodontic origin
title_fullStr Bone resorptive activity in symptomatic and asymptomatic apical lesions of endodontic origin
title_full_unstemmed Bone resorptive activity in symptomatic and asymptomatic apical lesions of endodontic origin
title_short Bone resorptive activity in symptomatic and asymptomatic apical lesions of endodontic origin
title_sort bone resorptive activity in symptomatic and asymptomatic apical lesions of endodontic origin
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5632344/
https://www.ncbi.nlm.nih.gov/pubmed/28261746
http://dx.doi.org/10.1007/s00784-017-2062-x
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