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Molecular evidence of Chlamydia pecorum and arthropod-associated Chlamydiae in an expanded range of marsupials
The order Chlamydiales are biphasic intracellular bacterial pathogens infecting humans and domesticated animals. Wildlife infections have also been reported, with the most studied example being Chlamydia pecorum infections in the koala, an iconic Australian marsupial. In koalas, molecular evidence s...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5634461/ https://www.ncbi.nlm.nih.gov/pubmed/28993660 http://dx.doi.org/10.1038/s41598-017-13164-y |
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author | Burnard, Delaney Huston, Wilhelmina M. Webb, Jonathan K. Jelocnik, Martina Reiss, Andrea Gillett, Amber Fitzgibbon, Sean Carver, Scott Carrucan, Janine Flanagan, Cheyne Timms, Peter Polkinghorne, Adam |
author_facet | Burnard, Delaney Huston, Wilhelmina M. Webb, Jonathan K. Jelocnik, Martina Reiss, Andrea Gillett, Amber Fitzgibbon, Sean Carver, Scott Carrucan, Janine Flanagan, Cheyne Timms, Peter Polkinghorne, Adam |
author_sort | Burnard, Delaney |
collection | PubMed |
description | The order Chlamydiales are biphasic intracellular bacterial pathogens infecting humans and domesticated animals. Wildlife infections have also been reported, with the most studied example being Chlamydia pecorum infections in the koala, an iconic Australian marsupial. In koalas, molecular evidence suggests that spill-over from C. pecorum infected livestock imported into Australia may have had a historical or contemporary role. Despite preliminary evidence that other native Australian marsupials also carry C. pecorum, their potential as reservoirs of this pathogen and other Chlamydia-related bacteria (CRBs) has been understudied. Mucosal epithelial samples collected from over 200 native Australian marsupials of different species and geographic regions across Australia were PCR screened for Chlamydiales. Previously described and genetically distinct C. pecorum genotypes and a range of 16S rRNA genotypes sharing similarity to different CRBs in the broader Chlamydiales order were present. One 16S rRNA Chlamydiales genotype recently described in Australian ticks that parasitise native Australian marsupials was also identified. This study provides further evidence that chlamydial infections are widespread in native fauna and that detailed investigations are required to understand the influence these infections have on host species conservation, but also whether infection spill-over plays a role in their epidemiology. |
format | Online Article Text |
id | pubmed-5634461 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-56344612017-10-18 Molecular evidence of Chlamydia pecorum and arthropod-associated Chlamydiae in an expanded range of marsupials Burnard, Delaney Huston, Wilhelmina M. Webb, Jonathan K. Jelocnik, Martina Reiss, Andrea Gillett, Amber Fitzgibbon, Sean Carver, Scott Carrucan, Janine Flanagan, Cheyne Timms, Peter Polkinghorne, Adam Sci Rep Article The order Chlamydiales are biphasic intracellular bacterial pathogens infecting humans and domesticated animals. Wildlife infections have also been reported, with the most studied example being Chlamydia pecorum infections in the koala, an iconic Australian marsupial. In koalas, molecular evidence suggests that spill-over from C. pecorum infected livestock imported into Australia may have had a historical or contemporary role. Despite preliminary evidence that other native Australian marsupials also carry C. pecorum, their potential as reservoirs of this pathogen and other Chlamydia-related bacteria (CRBs) has been understudied. Mucosal epithelial samples collected from over 200 native Australian marsupials of different species and geographic regions across Australia were PCR screened for Chlamydiales. Previously described and genetically distinct C. pecorum genotypes and a range of 16S rRNA genotypes sharing similarity to different CRBs in the broader Chlamydiales order were present. One 16S rRNA Chlamydiales genotype recently described in Australian ticks that parasitise native Australian marsupials was also identified. This study provides further evidence that chlamydial infections are widespread in native fauna and that detailed investigations are required to understand the influence these infections have on host species conservation, but also whether infection spill-over plays a role in their epidemiology. Nature Publishing Group UK 2017-10-09 /pmc/articles/PMC5634461/ /pubmed/28993660 http://dx.doi.org/10.1038/s41598-017-13164-y Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Burnard, Delaney Huston, Wilhelmina M. Webb, Jonathan K. Jelocnik, Martina Reiss, Andrea Gillett, Amber Fitzgibbon, Sean Carver, Scott Carrucan, Janine Flanagan, Cheyne Timms, Peter Polkinghorne, Adam Molecular evidence of Chlamydia pecorum and arthropod-associated Chlamydiae in an expanded range of marsupials |
title | Molecular evidence of Chlamydia pecorum and arthropod-associated Chlamydiae in an expanded range of marsupials |
title_full | Molecular evidence of Chlamydia pecorum and arthropod-associated Chlamydiae in an expanded range of marsupials |
title_fullStr | Molecular evidence of Chlamydia pecorum and arthropod-associated Chlamydiae in an expanded range of marsupials |
title_full_unstemmed | Molecular evidence of Chlamydia pecorum and arthropod-associated Chlamydiae in an expanded range of marsupials |
title_short | Molecular evidence of Chlamydia pecorum and arthropod-associated Chlamydiae in an expanded range of marsupials |
title_sort | molecular evidence of chlamydia pecorum and arthropod-associated chlamydiae in an expanded range of marsupials |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5634461/ https://www.ncbi.nlm.nih.gov/pubmed/28993660 http://dx.doi.org/10.1038/s41598-017-13164-y |
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