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Generation of beta-lactoglobulin knock-out goats using CRISPR/Cas9

Goat’s milk, considered a substitute for cow’s milk, has a high nutritional value. However, goat’s milk contains various allergens, predominantly β-lactoglobulin (BLG). In this study, we employed the CRISPR/Cas9 system to target the BLG locus in goat fibroblasts for sgRNA optimization and generate B...

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Detalles Bibliográficos
Autores principales:	Zhou, Wenjun, Wan, Yongjie, Guo, Rihong, Deng, Mingtian, Deng, Kaiping, Wang, Zhen, Zhang, Yanli, Wang, Feng
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2017
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5634636/ https://www.ncbi.nlm.nih.gov/pubmed/29016691 http://dx.doi.org/10.1371/journal.pone.0186056

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