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A viral protein promotes host SAMS1 activity and ethylene production for the benefit of virus infection

Ethylene plays critical roles in plant development and biotic stress response, but the mechanism of ethylene in host antiviral response remains unclear. Here, we report that Rice dwarf virus (RDV) triggers ethylene production by stimulating the activity of S-adenosyl-L-methionine synthetase (SAMS),...

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Autores principales: Zhao, Shanshan, Hong, Wei, Wu, Jianguo, Wang, Yu, Ji, Shaoyi, Zhu, Shuyi, Wei, Chunhong, Zhang, Jinsong, Li, Yi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5634785/
https://www.ncbi.nlm.nih.gov/pubmed/28994391
http://dx.doi.org/10.7554/eLife.27529
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author Zhao, Shanshan
Hong, Wei
Wu, Jianguo
Wang, Yu
Ji, Shaoyi
Zhu, Shuyi
Wei, Chunhong
Zhang, Jinsong
Li, Yi
author_facet Zhao, Shanshan
Hong, Wei
Wu, Jianguo
Wang, Yu
Ji, Shaoyi
Zhu, Shuyi
Wei, Chunhong
Zhang, Jinsong
Li, Yi
author_sort Zhao, Shanshan
collection PubMed
description Ethylene plays critical roles in plant development and biotic stress response, but the mechanism of ethylene in host antiviral response remains unclear. Here, we report that Rice dwarf virus (RDV) triggers ethylene production by stimulating the activity of S-adenosyl-L-methionine synthetase (SAMS), a key component of the ethylene synthesis pathway, resulting in elevated susceptibility to RDV. RDV-encoded Pns11 protein specifically interacted with OsSAMS1 to enhance its enzymatic activity, leading to higher ethylene levels in both RDV-infected and Pns11-overexpressing rice. Consistent with a counter-defense role for ethylene, Pns11-overexpressing rice, as well as those overexpressing OsSAMS1, were substantially more susceptible to RDV infection, and a similar effect was observed in rice plants treated with an ethylene precursor. Conversely, OsSAMS1-knockout mutants, as well as an osein2 mutant defective in ethylene signaling, resisted RDV infection more robustly. Our findings uncover a novel mechanism which RDV manipulates ethylene biosynthesis in the host plants to achieve efficient infection.
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spelling pubmed-56347852017-10-12 A viral protein promotes host SAMS1 activity and ethylene production for the benefit of virus infection Zhao, Shanshan Hong, Wei Wu, Jianguo Wang, Yu Ji, Shaoyi Zhu, Shuyi Wei, Chunhong Zhang, Jinsong Li, Yi eLife Plant Biology Ethylene plays critical roles in plant development and biotic stress response, but the mechanism of ethylene in host antiviral response remains unclear. Here, we report that Rice dwarf virus (RDV) triggers ethylene production by stimulating the activity of S-adenosyl-L-methionine synthetase (SAMS), a key component of the ethylene synthesis pathway, resulting in elevated susceptibility to RDV. RDV-encoded Pns11 protein specifically interacted with OsSAMS1 to enhance its enzymatic activity, leading to higher ethylene levels in both RDV-infected and Pns11-overexpressing rice. Consistent with a counter-defense role for ethylene, Pns11-overexpressing rice, as well as those overexpressing OsSAMS1, were substantially more susceptible to RDV infection, and a similar effect was observed in rice plants treated with an ethylene precursor. Conversely, OsSAMS1-knockout mutants, as well as an osein2 mutant defective in ethylene signaling, resisted RDV infection more robustly. Our findings uncover a novel mechanism which RDV manipulates ethylene biosynthesis in the host plants to achieve efficient infection. eLife Sciences Publications, Ltd 2017-10-10 /pmc/articles/PMC5634785/ /pubmed/28994391 http://dx.doi.org/10.7554/eLife.27529 Text en © 2017, Zhao et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Plant Biology
Zhao, Shanshan
Hong, Wei
Wu, Jianguo
Wang, Yu
Ji, Shaoyi
Zhu, Shuyi
Wei, Chunhong
Zhang, Jinsong
Li, Yi
A viral protein promotes host SAMS1 activity and ethylene production for the benefit of virus infection
title A viral protein promotes host SAMS1 activity and ethylene production for the benefit of virus infection
title_full A viral protein promotes host SAMS1 activity and ethylene production for the benefit of virus infection
title_fullStr A viral protein promotes host SAMS1 activity and ethylene production for the benefit of virus infection
title_full_unstemmed A viral protein promotes host SAMS1 activity and ethylene production for the benefit of virus infection
title_short A viral protein promotes host SAMS1 activity and ethylene production for the benefit of virus infection
title_sort viral protein promotes host sams1 activity and ethylene production for the benefit of virus infection
topic Plant Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5634785/
https://www.ncbi.nlm.nih.gov/pubmed/28994391
http://dx.doi.org/10.7554/eLife.27529
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