Evaluation of relative quantification of alternatively spliced transcripts using droplet digital PCR

INTRODUCTION: For the relative quantification of isoform expression, RT-qPCR has been the gold standard for over a decade. More recently, digital PCR is becoming widely implemented, as it is promised to be more accurate, sensitive and less affected by inhibitors, without the need for standard curves...

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Detalles Bibliográficos
Autores principales: Van Heetvelde, Mattias, Van Loocke, Wouter, Trypsteen, Wim, Baert, Annelot, Vanderheyden, Katrien, Crombez, Brecht, Vandesompele, Jo, De Leeneer, Kim, Claes, Kathleen B.M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2017
Materias:
Original Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5634819/
https://www.ncbi.nlm.nih.gov/pubmed/29021971
http://dx.doi.org/10.1016/j.bdq.2017.09.001

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