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CRISPR-Cas9 strategy for activation of silent Streptomyces biosynthetic gene clusters

Here we report an efficient CRISPR-Cas9 knock-in strategy to activate silent biosynthetic gene clusters (BGCs) in streptomycetes. We applied this one-step strategy to activate multiple BGCs of different classes in five Streptomyces species and triggered the production of unique metabolites, includin...

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Detalles Bibliográficos
Autores principales:	Zhang, Mingzi M, Wong, Fong Tian, Wang, Yajie, Luo, Shangwen, Lim, Yee Hwee, Heng, Elena, Yeo, Wan Lin, Cobb, Ryan E, Enghiad, Behnam, Ang, Ee Lui, Zhao, Huimin
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	2017
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5634907/ https://www.ncbi.nlm.nih.gov/pubmed/28398287 http://dx.doi.org/10.1038/nchembio.2341

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