Cargando…
High-throughput analysis unveils a highly shared satellite DNA library among three species of fish genus Astyanax
The high-throughput analysis of satellite DNA (satDNA) content, by means of Illumina sequencing, unveiled 45 satDNA families in the genome of Astyanax paranae, with repeat unit length (RUL) ranging from 6 to 365 bp and marked predominance of short satellites (median length = 59 bp). The analysis of...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5635008/ https://www.ncbi.nlm.nih.gov/pubmed/29018237 http://dx.doi.org/10.1038/s41598-017-12939-7 |
_version_ | 1783270194906398720 |
---|---|
author | Silva, Duílio M. Z. de A. Utsunomia, Ricardo Ruiz-Ruano, Francisco J. Daniel, Sandro Natal Porto-Foresti, Fábio Hashimoto, Diogo Teruo Oliveira, Claudio Camacho, Juan Pedro M. Foresti, Fausto |
author_facet | Silva, Duílio M. Z. de A. Utsunomia, Ricardo Ruiz-Ruano, Francisco J. Daniel, Sandro Natal Porto-Foresti, Fábio Hashimoto, Diogo Teruo Oliveira, Claudio Camacho, Juan Pedro M. Foresti, Fausto |
author_sort | Silva, Duílio M. Z. de A. |
collection | PubMed |
description | The high-throughput analysis of satellite DNA (satDNA) content, by means of Illumina sequencing, unveiled 45 satDNA families in the genome of Astyanax paranae, with repeat unit length (RUL) ranging from 6 to 365 bp and marked predominance of short satellites (median length = 59 bp). The analysis of chromosomal location of 35 satDNAs in A. paranae, A. fasciatus and A. bockmanni revealed that most satellites are shared between the three species and show highly similar patterns of chromosome distribution. The high similarity in satellite DNA content between these species is most likely due to their recent common descent. Among the few differences found, the ApaSat44-21 satellite was present only on the B chromosome of A. paranae, but not on the A or B chromosomes of the two other species. Likewise, the ApaSat20-18 satellite was B-specific in A. paranae but was however present on A and B chromosomes of A. fasciatus and A. bockmanni. The isochromosome nature of B chromosomes in these species was evidenced by the symmetric location of many satDNAs on both B chromosome arms, and the lower symmetry observed in the A. fasciatus BfMa chromosome suggests that it is older than those analyzed in A. paranae and A. bockmanni. |
format | Online Article Text |
id | pubmed-5635008 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-56350082017-10-18 High-throughput analysis unveils a highly shared satellite DNA library among three species of fish genus Astyanax Silva, Duílio M. Z. de A. Utsunomia, Ricardo Ruiz-Ruano, Francisco J. Daniel, Sandro Natal Porto-Foresti, Fábio Hashimoto, Diogo Teruo Oliveira, Claudio Camacho, Juan Pedro M. Foresti, Fausto Sci Rep Article The high-throughput analysis of satellite DNA (satDNA) content, by means of Illumina sequencing, unveiled 45 satDNA families in the genome of Astyanax paranae, with repeat unit length (RUL) ranging from 6 to 365 bp and marked predominance of short satellites (median length = 59 bp). The analysis of chromosomal location of 35 satDNAs in A. paranae, A. fasciatus and A. bockmanni revealed that most satellites are shared between the three species and show highly similar patterns of chromosome distribution. The high similarity in satellite DNA content between these species is most likely due to their recent common descent. Among the few differences found, the ApaSat44-21 satellite was present only on the B chromosome of A. paranae, but not on the A or B chromosomes of the two other species. Likewise, the ApaSat20-18 satellite was B-specific in A. paranae but was however present on A and B chromosomes of A. fasciatus and A. bockmanni. The isochromosome nature of B chromosomes in these species was evidenced by the symmetric location of many satDNAs on both B chromosome arms, and the lower symmetry observed in the A. fasciatus BfMa chromosome suggests that it is older than those analyzed in A. paranae and A. bockmanni. Nature Publishing Group UK 2017-10-05 /pmc/articles/PMC5635008/ /pubmed/29018237 http://dx.doi.org/10.1038/s41598-017-12939-7 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Silva, Duílio M. Z. de A. Utsunomia, Ricardo Ruiz-Ruano, Francisco J. Daniel, Sandro Natal Porto-Foresti, Fábio Hashimoto, Diogo Teruo Oliveira, Claudio Camacho, Juan Pedro M. Foresti, Fausto High-throughput analysis unveils a highly shared satellite DNA library among three species of fish genus Astyanax |
title | High-throughput analysis unveils a highly shared satellite DNA library among three species of fish genus Astyanax |
title_full | High-throughput analysis unveils a highly shared satellite DNA library among three species of fish genus Astyanax |
title_fullStr | High-throughput analysis unveils a highly shared satellite DNA library among three species of fish genus Astyanax |
title_full_unstemmed | High-throughput analysis unveils a highly shared satellite DNA library among three species of fish genus Astyanax |
title_short | High-throughput analysis unveils a highly shared satellite DNA library among three species of fish genus Astyanax |
title_sort | high-throughput analysis unveils a highly shared satellite dna library among three species of fish genus astyanax |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5635008/ https://www.ncbi.nlm.nih.gov/pubmed/29018237 http://dx.doi.org/10.1038/s41598-017-12939-7 |
work_keys_str_mv | AT silvaduiliomzdea highthroughputanalysisunveilsahighlysharedsatellitednalibraryamongthreespeciesoffishgenusastyanax AT utsunomiaricardo highthroughputanalysisunveilsahighlysharedsatellitednalibraryamongthreespeciesoffishgenusastyanax AT ruizruanofranciscoj highthroughputanalysisunveilsahighlysharedsatellitednalibraryamongthreespeciesoffishgenusastyanax AT danielsandronatal highthroughputanalysisunveilsahighlysharedsatellitednalibraryamongthreespeciesoffishgenusastyanax AT portoforestifabio highthroughputanalysisunveilsahighlysharedsatellitednalibraryamongthreespeciesoffishgenusastyanax AT hashimotodiogoteruo highthroughputanalysisunveilsahighlysharedsatellitednalibraryamongthreespeciesoffishgenusastyanax AT oliveiraclaudio highthroughputanalysisunveilsahighlysharedsatellitednalibraryamongthreespeciesoffishgenusastyanax AT camachojuanpedrom highthroughputanalysisunveilsahighlysharedsatellitednalibraryamongthreespeciesoffishgenusastyanax AT forestifausto highthroughputanalysisunveilsahighlysharedsatellitednalibraryamongthreespeciesoffishgenusastyanax |