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Potential biotechnological capabilities of cultivable mycobiota from carwash effluents

Urban life has created man‐made extreme environments like carwashes. These environments have, however, not been sufficiently explored for mycobiota that can be sources of biotechnologically useful products, as has been the case with natural extreme environments. Using a combination of culture and mo...

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Autores principales: Sibanda, Timothy, Selvarajan, Ramganesh, Tekere, Memory, Nyoni, Hlengilizwe, Meddows‐Taylor, Stephen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5635173/
https://www.ncbi.nlm.nih.gov/pubmed/28714266
http://dx.doi.org/10.1002/mbo3.498
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author Sibanda, Timothy
Selvarajan, Ramganesh
Tekere, Memory
Nyoni, Hlengilizwe
Meddows‐Taylor, Stephen
author_facet Sibanda, Timothy
Selvarajan, Ramganesh
Tekere, Memory
Nyoni, Hlengilizwe
Meddows‐Taylor, Stephen
author_sort Sibanda, Timothy
collection PubMed
description Urban life has created man‐made extreme environments like carwashes. These environments have, however, not been sufficiently explored for mycobiota that can be sources of biotechnologically useful products, as has been the case with natural extreme environments. Using a combination of culture and molecular techniques, fungi from carwash effluents was characterized for production of lipase and cellulase enzymes, nonpolar and polar biotechnologically relevant secondary metabolites and hydrocarbon utilization. The isolated fungal strains belonged to the genera Alternaria, Cladosporium, Penicillium, Peyronellaea, Rhizopus, Spegazzinia, Trichoderma, Ulocladium and Yarrowia. Sixty‐six percent (66%) of the fungal isolates were found to be able to metabolize naphthalene and benzanthracene, showing potential for application in bioremediation of hydrocarbon polluted sites. Lipase production by the isolates Penicillium sp. BPS3 (2.61 U/ml), Trichoderma sp. BPS9 (2.01 U/ml), Rhizopus sp. CAL1 (2.05 U/ml), Penicillium sp. PCW1 (2.99 U/ml) and Penicillium sp. SAS1 (2.16 U/ml) compared well with previously recorded lipase production levels by other fungi. The highest producers of cellulase were Penicillium sp. SAS1 (12.10 U/ml), Peyronella sp. CAW5 (4.49 U/ml) and Cladosporium sp. SAS3 (4.07 U/ml), although these activities were lower than previously reported levels. GC‐MS analysis of the fungal secondary metabolites resulted in identification of 572 compounds, including azulene, methanamine, N‐pentylidene, metoclopramide, and mepivacaine while compounds determined by UHPLC‐MS included 10‐undecen‐1‐ol, piquerol A, 10‐undecyn‐1‐ol, cyclo(leucylprolyl) and rac‐etomidate. These compounds were previously determined to have various activities including anticancer, antibacterial, antifungal, antihypertensive, antidiabetic and anti‐inflammatory properties. The study demonstrated that fungi from carwash effluents are natural sources of some biotechnologically important products.
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spelling pubmed-56351732017-10-18 Potential biotechnological capabilities of cultivable mycobiota from carwash effluents Sibanda, Timothy Selvarajan, Ramganesh Tekere, Memory Nyoni, Hlengilizwe Meddows‐Taylor, Stephen Microbiologyopen Original Research Urban life has created man‐made extreme environments like carwashes. These environments have, however, not been sufficiently explored for mycobiota that can be sources of biotechnologically useful products, as has been the case with natural extreme environments. Using a combination of culture and molecular techniques, fungi from carwash effluents was characterized for production of lipase and cellulase enzymes, nonpolar and polar biotechnologically relevant secondary metabolites and hydrocarbon utilization. The isolated fungal strains belonged to the genera Alternaria, Cladosporium, Penicillium, Peyronellaea, Rhizopus, Spegazzinia, Trichoderma, Ulocladium and Yarrowia. Sixty‐six percent (66%) of the fungal isolates were found to be able to metabolize naphthalene and benzanthracene, showing potential for application in bioremediation of hydrocarbon polluted sites. Lipase production by the isolates Penicillium sp. BPS3 (2.61 U/ml), Trichoderma sp. BPS9 (2.01 U/ml), Rhizopus sp. CAL1 (2.05 U/ml), Penicillium sp. PCW1 (2.99 U/ml) and Penicillium sp. SAS1 (2.16 U/ml) compared well with previously recorded lipase production levels by other fungi. The highest producers of cellulase were Penicillium sp. SAS1 (12.10 U/ml), Peyronella sp. CAW5 (4.49 U/ml) and Cladosporium sp. SAS3 (4.07 U/ml), although these activities were lower than previously reported levels. GC‐MS analysis of the fungal secondary metabolites resulted in identification of 572 compounds, including azulene, methanamine, N‐pentylidene, metoclopramide, and mepivacaine while compounds determined by UHPLC‐MS included 10‐undecen‐1‐ol, piquerol A, 10‐undecyn‐1‐ol, cyclo(leucylprolyl) and rac‐etomidate. These compounds were previously determined to have various activities including anticancer, antibacterial, antifungal, antihypertensive, antidiabetic and anti‐inflammatory properties. The study demonstrated that fungi from carwash effluents are natural sources of some biotechnologically important products. John Wiley and Sons Inc. 2017-07-17 /pmc/articles/PMC5635173/ /pubmed/28714266 http://dx.doi.org/10.1002/mbo3.498 Text en © 2017 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Sibanda, Timothy
Selvarajan, Ramganesh
Tekere, Memory
Nyoni, Hlengilizwe
Meddows‐Taylor, Stephen
Potential biotechnological capabilities of cultivable mycobiota from carwash effluents
title Potential biotechnological capabilities of cultivable mycobiota from carwash effluents
title_full Potential biotechnological capabilities of cultivable mycobiota from carwash effluents
title_fullStr Potential biotechnological capabilities of cultivable mycobiota from carwash effluents
title_full_unstemmed Potential biotechnological capabilities of cultivable mycobiota from carwash effluents
title_short Potential biotechnological capabilities of cultivable mycobiota from carwash effluents
title_sort potential biotechnological capabilities of cultivable mycobiota from carwash effluents
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5635173/
https://www.ncbi.nlm.nih.gov/pubmed/28714266
http://dx.doi.org/10.1002/mbo3.498
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