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First Evidence for the Disease-Stage, Cell-Type, and Virus Specificity of microRNAs during Human Immunodeficiency Virus Type-1 Infection

The potential involvement of host microRNAs (miRNAs) in HIV infection is well documented, and evidence suggests that HIV modulates and also dysregulates host miRNAs involved in maintaining the host innate immune system. Moreover, the dysregulation of host miRNAs by HIV also effectively interferes di...

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Autores principales: Fowler, Lauren, Conceicao, Viviane, Perera, Suneth S., Gupta, Priyanka, Chew, Choo Beng, Dyer, Wayne B., Saksena, Nitin K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5635779/
https://www.ncbi.nlm.nih.gov/pubmed/29083374
http://dx.doi.org/10.3390/medsci4020010
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author Fowler, Lauren
Conceicao, Viviane
Perera, Suneth S.
Gupta, Priyanka
Chew, Choo Beng
Dyer, Wayne B.
Saksena, Nitin K.
author_facet Fowler, Lauren
Conceicao, Viviane
Perera, Suneth S.
Gupta, Priyanka
Chew, Choo Beng
Dyer, Wayne B.
Saksena, Nitin K.
author_sort Fowler, Lauren
collection PubMed
description The potential involvement of host microRNAs (miRNAs) in HIV infection is well documented, and evidence suggests that HIV modulates and also dysregulates host miRNAs involved in maintaining the host innate immune system. Moreover, the dysregulation of host miRNAs by HIV also effectively interferes directly with the host gene expression. In this study, we have simultaneously evaluated the expression of host miRNAs in both CD4+ and CD8+ T-cells derived from HIV-positive (HIV+) individuals (viremic and aviremic individuals while receiving highly active antiretroviral therapy (HAART), therapy-naïve long-term non-progressors (LTNP), and HIV-negative (HIV–) healthy controls. miRNAs were run on Affymetrix V2 chips, and the differential expression between HIV+ and HIV− samples, along with intergroup comparisons, was derived using PARTEK software, using an FDR of 5% and an adjusted p-value < 0.05. The miR-199a-5p was found to be HIV-specific and expressed in all HIV+ groups as opposed to HIV– controls. Moreover, these are the first studies to reveal clearly the highly discriminatory miRNAs at the level of the disease state, cell type, and HIV-specific miRNAs.
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spelling pubmed-56357792017-10-26 First Evidence for the Disease-Stage, Cell-Type, and Virus Specificity of microRNAs during Human Immunodeficiency Virus Type-1 Infection Fowler, Lauren Conceicao, Viviane Perera, Suneth S. Gupta, Priyanka Chew, Choo Beng Dyer, Wayne B. Saksena, Nitin K. Med Sci (Basel) Article The potential involvement of host microRNAs (miRNAs) in HIV infection is well documented, and evidence suggests that HIV modulates and also dysregulates host miRNAs involved in maintaining the host innate immune system. Moreover, the dysregulation of host miRNAs by HIV also effectively interferes directly with the host gene expression. In this study, we have simultaneously evaluated the expression of host miRNAs in both CD4+ and CD8+ T-cells derived from HIV-positive (HIV+) individuals (viremic and aviremic individuals while receiving highly active antiretroviral therapy (HAART), therapy-naïve long-term non-progressors (LTNP), and HIV-negative (HIV–) healthy controls. miRNAs were run on Affymetrix V2 chips, and the differential expression between HIV+ and HIV− samples, along with intergroup comparisons, was derived using PARTEK software, using an FDR of 5% and an adjusted p-value < 0.05. The miR-199a-5p was found to be HIV-specific and expressed in all HIV+ groups as opposed to HIV– controls. Moreover, these are the first studies to reveal clearly the highly discriminatory miRNAs at the level of the disease state, cell type, and HIV-specific miRNAs. MDPI 2016-05-13 /pmc/articles/PMC5635779/ /pubmed/29083374 http://dx.doi.org/10.3390/medsci4020010 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Fowler, Lauren
Conceicao, Viviane
Perera, Suneth S.
Gupta, Priyanka
Chew, Choo Beng
Dyer, Wayne B.
Saksena, Nitin K.
First Evidence for the Disease-Stage, Cell-Type, and Virus Specificity of microRNAs during Human Immunodeficiency Virus Type-1 Infection
title First Evidence for the Disease-Stage, Cell-Type, and Virus Specificity of microRNAs during Human Immunodeficiency Virus Type-1 Infection
title_full First Evidence for the Disease-Stage, Cell-Type, and Virus Specificity of microRNAs during Human Immunodeficiency Virus Type-1 Infection
title_fullStr First Evidence for the Disease-Stage, Cell-Type, and Virus Specificity of microRNAs during Human Immunodeficiency Virus Type-1 Infection
title_full_unstemmed First Evidence for the Disease-Stage, Cell-Type, and Virus Specificity of microRNAs during Human Immunodeficiency Virus Type-1 Infection
title_short First Evidence for the Disease-Stage, Cell-Type, and Virus Specificity of microRNAs during Human Immunodeficiency Virus Type-1 Infection
title_sort first evidence for the disease-stage, cell-type, and virus specificity of micrornas during human immunodeficiency virus type-1 infection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5635779/
https://www.ncbi.nlm.nih.gov/pubmed/29083374
http://dx.doi.org/10.3390/medsci4020010
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