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Molecular Characterization of Peripheral Extracellular Vesicles in Clinically Isolated Syndrome: Preliminary Suggestions from a Pilot Study

Extracellular vesicles (EVs), nanoparticles originated from different cell types, seem to be implicated in several cellular activities. In the Central Nervous System (CNS), glia and neurons secrete EVs and recent studies have demonstrated that the intercellular communication mediated by EVs has vers...

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Autores principales: Nuzziello, Nicoletta, Blonda, Maria, Licciulli, Flavio, Liuni, Sabino, Amoruso, Antonella, Valletti, Alessio, Consiglio, Arianna, Avolio, Carlo, Liguori, Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5635803/
https://www.ncbi.nlm.nih.gov/pubmed/29099035
http://dx.doi.org/10.3390/medsci5030019
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author Nuzziello, Nicoletta
Blonda, Maria
Licciulli, Flavio
Liuni, Sabino
Amoruso, Antonella
Valletti, Alessio
Consiglio, Arianna
Avolio, Carlo
Liguori, Maria
author_facet Nuzziello, Nicoletta
Blonda, Maria
Licciulli, Flavio
Liuni, Sabino
Amoruso, Antonella
Valletti, Alessio
Consiglio, Arianna
Avolio, Carlo
Liguori, Maria
author_sort Nuzziello, Nicoletta
collection PubMed
description Extracellular vesicles (EVs), nanoparticles originated from different cell types, seem to be implicated in several cellular activities. In the Central Nervous System (CNS), glia and neurons secrete EVs and recent studies have demonstrated that the intercellular communication mediated by EVs has versatile functional impact in the cerebral homeostasis. This essential role may be due to their proteins and RNAs cargo that possibly modify the phenotypes of the targeted cells. Despite the increasing importance of EVs, little is known about their fluctuations in physiological as well as in pathological conditions. Furthermore, only few studies have investigated the contents of contemporary EVs subgroups (microvesicles, MVs and exosomes, EXOs) with the purpose of discriminating between their features and functional roles. In order to possibly shed light on these issues, we performed a pilot study in which MVs and EXOs extracted from serum samples of a little cohort of subjects (patients with the first clinical evidence of CNS demyelination, also known as Clinically Isolated Syndrome and Healthy Controls) were submitted to deep small-RNA sequencing. Data were analysed by an in-home bioinformatics platform. In line with previous reports, distinct classes of non-coding RNAs have been detected in both the EVs subsets, offering interesting suggestions on their origins and functions. We also verified the feasibility of this extensive molecular approach, thus supporting its valuable use for the analysis of circulating biomarkers (e.g., microRNAs) in order to investigate and monitor specific diseases.
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spelling pubmed-56358032017-10-26 Molecular Characterization of Peripheral Extracellular Vesicles in Clinically Isolated Syndrome: Preliminary Suggestions from a Pilot Study Nuzziello, Nicoletta Blonda, Maria Licciulli, Flavio Liuni, Sabino Amoruso, Antonella Valletti, Alessio Consiglio, Arianna Avolio, Carlo Liguori, Maria Med Sci (Basel) Communication Extracellular vesicles (EVs), nanoparticles originated from different cell types, seem to be implicated in several cellular activities. In the Central Nervous System (CNS), glia and neurons secrete EVs and recent studies have demonstrated that the intercellular communication mediated by EVs has versatile functional impact in the cerebral homeostasis. This essential role may be due to their proteins and RNAs cargo that possibly modify the phenotypes of the targeted cells. Despite the increasing importance of EVs, little is known about their fluctuations in physiological as well as in pathological conditions. Furthermore, only few studies have investigated the contents of contemporary EVs subgroups (microvesicles, MVs and exosomes, EXOs) with the purpose of discriminating between their features and functional roles. In order to possibly shed light on these issues, we performed a pilot study in which MVs and EXOs extracted from serum samples of a little cohort of subjects (patients with the first clinical evidence of CNS demyelination, also known as Clinically Isolated Syndrome and Healthy Controls) were submitted to deep small-RNA sequencing. Data were analysed by an in-home bioinformatics platform. In line with previous reports, distinct classes of non-coding RNAs have been detected in both the EVs subsets, offering interesting suggestions on their origins and functions. We also verified the feasibility of this extensive molecular approach, thus supporting its valuable use for the analysis of circulating biomarkers (e.g., microRNAs) in order to investigate and monitor specific diseases. MDPI 2017-09-18 /pmc/articles/PMC5635803/ /pubmed/29099035 http://dx.doi.org/10.3390/medsci5030019 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Nuzziello, Nicoletta
Blonda, Maria
Licciulli, Flavio
Liuni, Sabino
Amoruso, Antonella
Valletti, Alessio
Consiglio, Arianna
Avolio, Carlo
Liguori, Maria
Molecular Characterization of Peripheral Extracellular Vesicles in Clinically Isolated Syndrome: Preliminary Suggestions from a Pilot Study
title Molecular Characterization of Peripheral Extracellular Vesicles in Clinically Isolated Syndrome: Preliminary Suggestions from a Pilot Study
title_full Molecular Characterization of Peripheral Extracellular Vesicles in Clinically Isolated Syndrome: Preliminary Suggestions from a Pilot Study
title_fullStr Molecular Characterization of Peripheral Extracellular Vesicles in Clinically Isolated Syndrome: Preliminary Suggestions from a Pilot Study
title_full_unstemmed Molecular Characterization of Peripheral Extracellular Vesicles in Clinically Isolated Syndrome: Preliminary Suggestions from a Pilot Study
title_short Molecular Characterization of Peripheral Extracellular Vesicles in Clinically Isolated Syndrome: Preliminary Suggestions from a Pilot Study
title_sort molecular characterization of peripheral extracellular vesicles in clinically isolated syndrome: preliminary suggestions from a pilot study
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5635803/
https://www.ncbi.nlm.nih.gov/pubmed/29099035
http://dx.doi.org/10.3390/medsci5030019
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