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Susceptibility of different mouse strains to oxaliplatin peripheral neurotoxicity: Phenotypic and genotypic insights
Peripheral neurotoxicity is one of the most distressing side effects of oxaliplatin therapy for cancer. Indeed, most patients that received oxaliplatin experience acute and/or chronic severe sensory peripheral neuropathy. However, despite similar co-morbidities, cancer stage, demographics and treatm...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5636145/ https://www.ncbi.nlm.nih.gov/pubmed/29020118 http://dx.doi.org/10.1371/journal.pone.0186250 |
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author | Marmiroli, Paola Riva, Beatrice Pozzi, Eleonora Ballarini, Elisa Lim, Dmitry Chiorazzi, Alessia Meregalli, Cristina Distasi, Carla Renn, Cynthia L. Semperboni, Sara Morosi, Lavinia Ruffinatti, Federico A. Zucchetti, Massimo Dorsey, Susan G. Cavaletti, Guido Genazzani, Armando Carozzi, Valentina A. |
author_facet | Marmiroli, Paola Riva, Beatrice Pozzi, Eleonora Ballarini, Elisa Lim, Dmitry Chiorazzi, Alessia Meregalli, Cristina Distasi, Carla Renn, Cynthia L. Semperboni, Sara Morosi, Lavinia Ruffinatti, Federico A. Zucchetti, Massimo Dorsey, Susan G. Cavaletti, Guido Genazzani, Armando Carozzi, Valentina A. |
author_sort | Marmiroli, Paola |
collection | PubMed |
description | Peripheral neurotoxicity is one of the most distressing side effects of oxaliplatin therapy for cancer. Indeed, most patients that received oxaliplatin experience acute and/or chronic severe sensory peripheral neuropathy. However, despite similar co-morbidities, cancer stage, demographics and treatment schedule, patients develop oxaliplatin-induced peripheral neurotoxicity with remarkably different severity. This suggests individual genetic variability, which might be used to glean the mechanistic insights into oxaliplatin neurotoxicity. We characterized the susceptibility of different mice strains to oxaliplatin neurotoxicity investigating the phenotypic features of neuropathy and gene expression profiles in dorsal root ganglia of six genetically different mice strains (Balb-c, C57BL6, DBA/2J, AJ, FVB and CD1) exposed to the same oxaliplatin schedule. Differential gene expression in dorsal root ganglia from each mice strain were assayed using a genome-wide expression analysis and selected genes were validated by RT-PCR analysis. The demonstration of consistent differences in the phenotypic response to oxaliplatin across different strains is interesting to allow the selection of the appropriate strain based on the pre-defined read-out parameters. Further investigation of the correlation between gene expression changes and oxaliplatin-induced neurotoxicity phenotype in each strain will be useful to deeper investigate the molecular mechanisms of oxaliplatin neurotoxicity. |
format | Online Article Text |
id | pubmed-5636145 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-56361452017-10-30 Susceptibility of different mouse strains to oxaliplatin peripheral neurotoxicity: Phenotypic and genotypic insights Marmiroli, Paola Riva, Beatrice Pozzi, Eleonora Ballarini, Elisa Lim, Dmitry Chiorazzi, Alessia Meregalli, Cristina Distasi, Carla Renn, Cynthia L. Semperboni, Sara Morosi, Lavinia Ruffinatti, Federico A. Zucchetti, Massimo Dorsey, Susan G. Cavaletti, Guido Genazzani, Armando Carozzi, Valentina A. PLoS One Research Article Peripheral neurotoxicity is one of the most distressing side effects of oxaliplatin therapy for cancer. Indeed, most patients that received oxaliplatin experience acute and/or chronic severe sensory peripheral neuropathy. However, despite similar co-morbidities, cancer stage, demographics and treatment schedule, patients develop oxaliplatin-induced peripheral neurotoxicity with remarkably different severity. This suggests individual genetic variability, which might be used to glean the mechanistic insights into oxaliplatin neurotoxicity. We characterized the susceptibility of different mice strains to oxaliplatin neurotoxicity investigating the phenotypic features of neuropathy and gene expression profiles in dorsal root ganglia of six genetically different mice strains (Balb-c, C57BL6, DBA/2J, AJ, FVB and CD1) exposed to the same oxaliplatin schedule. Differential gene expression in dorsal root ganglia from each mice strain were assayed using a genome-wide expression analysis and selected genes were validated by RT-PCR analysis. The demonstration of consistent differences in the phenotypic response to oxaliplatin across different strains is interesting to allow the selection of the appropriate strain based on the pre-defined read-out parameters. Further investigation of the correlation between gene expression changes and oxaliplatin-induced neurotoxicity phenotype in each strain will be useful to deeper investigate the molecular mechanisms of oxaliplatin neurotoxicity. Public Library of Science 2017-10-11 /pmc/articles/PMC5636145/ /pubmed/29020118 http://dx.doi.org/10.1371/journal.pone.0186250 Text en © 2017 Marmiroli et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Marmiroli, Paola Riva, Beatrice Pozzi, Eleonora Ballarini, Elisa Lim, Dmitry Chiorazzi, Alessia Meregalli, Cristina Distasi, Carla Renn, Cynthia L. Semperboni, Sara Morosi, Lavinia Ruffinatti, Federico A. Zucchetti, Massimo Dorsey, Susan G. Cavaletti, Guido Genazzani, Armando Carozzi, Valentina A. Susceptibility of different mouse strains to oxaliplatin peripheral neurotoxicity: Phenotypic and genotypic insights |
title | Susceptibility of different mouse strains to oxaliplatin peripheral neurotoxicity: Phenotypic and genotypic insights |
title_full | Susceptibility of different mouse strains to oxaliplatin peripheral neurotoxicity: Phenotypic and genotypic insights |
title_fullStr | Susceptibility of different mouse strains to oxaliplatin peripheral neurotoxicity: Phenotypic and genotypic insights |
title_full_unstemmed | Susceptibility of different mouse strains to oxaliplatin peripheral neurotoxicity: Phenotypic and genotypic insights |
title_short | Susceptibility of different mouse strains to oxaliplatin peripheral neurotoxicity: Phenotypic and genotypic insights |
title_sort | susceptibility of different mouse strains to oxaliplatin peripheral neurotoxicity: phenotypic and genotypic insights |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5636145/ https://www.ncbi.nlm.nih.gov/pubmed/29020118 http://dx.doi.org/10.1371/journal.pone.0186250 |
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