Expression level of human TLR4 rather than sequence is the key determinant of LPS responsiveness

To address the role of Toll-like receptor 4 (TLR4) single nucleotide polymorphisms (SNP) in lipopolysaccharide (LPS) recognition, we generated mice that differed only in the sequence of TLR4. We used a bacterial artificial chromosome (BAC) transgenic approach and TLR4/MD-2 knockout mice to specifica...

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Autores principales: Hajjar, Adeline M., Ernst, Robert K., Yi, Jaehun, Yam, Cathy S., Miller, Samuel I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5636155/
https://www.ncbi.nlm.nih.gov/pubmed/29020088
http://dx.doi.org/10.1371/journal.pone.0186308
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author Hajjar, Adeline M.
Ernst, Robert K.
Yi, Jaehun
Yam, Cathy S.
Miller, Samuel I.
author_facet Hajjar, Adeline M.
Ernst, Robert K.
Yi, Jaehun
Yam, Cathy S.
Miller, Samuel I.
author_sort Hajjar, Adeline M.
collection PubMed
description To address the role of Toll-like receptor 4 (TLR4) single nucleotide polymorphisms (SNP) in lipopolysaccharide (LPS) recognition, we generated mice that differed only in the sequence of TLR4. We used a bacterial artificial chromosome (BAC) transgenic approach and TLR4/MD-2 knockout mice to specifically examine the role of human TLR4 variants in recognition of LPS. Using in vitro and in vivo assays we found that the expression level rather than the sequence of TLR4 played a larger role in recognition of LPS, especially hypoacylated LPS.
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spelling pubmed-56361552017-10-30 Expression level of human TLR4 rather than sequence is the key determinant of LPS responsiveness Hajjar, Adeline M. Ernst, Robert K. Yi, Jaehun Yam, Cathy S. Miller, Samuel I. PLoS One Research Article To address the role of Toll-like receptor 4 (TLR4) single nucleotide polymorphisms (SNP) in lipopolysaccharide (LPS) recognition, we generated mice that differed only in the sequence of TLR4. We used a bacterial artificial chromosome (BAC) transgenic approach and TLR4/MD-2 knockout mice to specifically examine the role of human TLR4 variants in recognition of LPS. Using in vitro and in vivo assays we found that the expression level rather than the sequence of TLR4 played a larger role in recognition of LPS, especially hypoacylated LPS. Public Library of Science 2017-10-11 /pmc/articles/PMC5636155/ /pubmed/29020088 http://dx.doi.org/10.1371/journal.pone.0186308 Text en © 2017 Hajjar et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Hajjar, Adeline M.
Ernst, Robert K.
Yi, Jaehun
Yam, Cathy S.
Miller, Samuel I.
Expression level of human TLR4 rather than sequence is the key determinant of LPS responsiveness
title Expression level of human TLR4 rather than sequence is the key determinant of LPS responsiveness
title_full Expression level of human TLR4 rather than sequence is the key determinant of LPS responsiveness
title_fullStr Expression level of human TLR4 rather than sequence is the key determinant of LPS responsiveness
title_full_unstemmed Expression level of human TLR4 rather than sequence is the key determinant of LPS responsiveness
title_short Expression level of human TLR4 rather than sequence is the key determinant of LPS responsiveness
title_sort expression level of human tlr4 rather than sequence is the key determinant of lps responsiveness
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5636155/
https://www.ncbi.nlm.nih.gov/pubmed/29020088
http://dx.doi.org/10.1371/journal.pone.0186308
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