Internally ratiometric fluorescent sensors for evaluation of intracellular GTP levels and distribution

GTP is a major regulator of multiple cellular processes, but tools for quantitative evaluation of GTP levels in live cells have not been available. Here we report characterization of genetically encoded GTP sensors, constructed by inserting cpYFP into a region of the bacterial FeoB G-protein that un...

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Detalles Bibliográficos
Autores principales: Bianchi-Smiraglia, Anna, Rana, Mitra S., Foley, Colleen E., Paul, Leslie M., Lipchick, Brittany C., Moparthy, Sudha, Moparthy, Kalyana, Fink, Emily E., Bagati, Archis, Hurley, Edward, Affronti, Hayley C., Bakin, Andrei V., Kandel, Eugene S., Smiraglia, Dominic J., Feltri, Maria Laura, Sousa, Rui, Nikiforov, Mikhail A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5636219/
https://www.ncbi.nlm.nih.gov/pubmed/28869758
http://dx.doi.org/10.1038/nmeth.4404

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5636219/
https://www.ncbi.nlm.nih.gov/pubmed/28869758
http://dx.doi.org/10.1038/nmeth.4404

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