Imaging mass spectrometry analysis of ubiquinol localization in the mouse brain following short-term administration

We analyzed the localization of ubiquinol, the reduced form of coenzyme Q10 (Re-CoQ10), in mouse brain sections using matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance imaging mass spectrometry (IMS) to evaluate the effect of dietary Re-CoQ10 in mouse brain. Mice...

Descripción completa

Detalles Bibliográficos
Autores principales: Tatsuta, Yukina, Kasai, Kazuaki, Maruyama, Chitose, Hamano, Yoshimitsu, Matsuo, Kazuhiko, Katano, Hajime, Taira, Shu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5636788/
https://www.ncbi.nlm.nih.gov/pubmed/29021617
http://dx.doi.org/10.1038/s41598-017-13257-8
_version_ 1783270507874877440
author Tatsuta, Yukina
Kasai, Kazuaki
Maruyama, Chitose
Hamano, Yoshimitsu
Matsuo, Kazuhiko
Katano, Hajime
Taira, Shu
author_facet Tatsuta, Yukina
Kasai, Kazuaki
Maruyama, Chitose
Hamano, Yoshimitsu
Matsuo, Kazuhiko
Katano, Hajime
Taira, Shu
author_sort Tatsuta, Yukina
collection PubMed
description We analyzed the localization of ubiquinol, the reduced form of coenzyme Q10 (Re-CoQ10), in mouse brain sections using matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance imaging mass spectrometry (IMS) to evaluate the effect of dietary Re-CoQ10 in mouse brain. Mice were orally administered Re-CoQ10 for 14 days and brain Re-CoQ10 content was subsequently quantified using liquid chromatography-mass spectrometry. IMS was employed to visualize Re-CoQ10 at a resolution of 150 μm in the mouse brain. Increased Re-CoQ10 was observed in the corpus callosum, hippocampus, midbrain, cerebellum, brain stem, substantia nigra and striatum. These regions are related to movement, memory and vital life functions. Thus, we demonstrated the effect of Re-CoQ10 administration on the specific localization of Re-CoQ10 in the brain.
format Online
Article
Text
id pubmed-5636788
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-56367882017-10-18 Imaging mass spectrometry analysis of ubiquinol localization in the mouse brain following short-term administration Tatsuta, Yukina Kasai, Kazuaki Maruyama, Chitose Hamano, Yoshimitsu Matsuo, Kazuhiko Katano, Hajime Taira, Shu Sci Rep Article We analyzed the localization of ubiquinol, the reduced form of coenzyme Q10 (Re-CoQ10), in mouse brain sections using matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance imaging mass spectrometry (IMS) to evaluate the effect of dietary Re-CoQ10 in mouse brain. Mice were orally administered Re-CoQ10 for 14 days and brain Re-CoQ10 content was subsequently quantified using liquid chromatography-mass spectrometry. IMS was employed to visualize Re-CoQ10 at a resolution of 150 μm in the mouse brain. Increased Re-CoQ10 was observed in the corpus callosum, hippocampus, midbrain, cerebellum, brain stem, substantia nigra and striatum. These regions are related to movement, memory and vital life functions. Thus, we demonstrated the effect of Re-CoQ10 administration on the specific localization of Re-CoQ10 in the brain. Nature Publishing Group UK 2017-10-11 /pmc/articles/PMC5636788/ /pubmed/29021617 http://dx.doi.org/10.1038/s41598-017-13257-8 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Tatsuta, Yukina
Kasai, Kazuaki
Maruyama, Chitose
Hamano, Yoshimitsu
Matsuo, Kazuhiko
Katano, Hajime
Taira, Shu
Imaging mass spectrometry analysis of ubiquinol localization in the mouse brain following short-term administration
title Imaging mass spectrometry analysis of ubiquinol localization in the mouse brain following short-term administration
title_full Imaging mass spectrometry analysis of ubiquinol localization in the mouse brain following short-term administration
title_fullStr Imaging mass spectrometry analysis of ubiquinol localization in the mouse brain following short-term administration
title_full_unstemmed Imaging mass spectrometry analysis of ubiquinol localization in the mouse brain following short-term administration
title_short Imaging mass spectrometry analysis of ubiquinol localization in the mouse brain following short-term administration
title_sort imaging mass spectrometry analysis of ubiquinol localization in the mouse brain following short-term administration
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5636788/
https://www.ncbi.nlm.nih.gov/pubmed/29021617
http://dx.doi.org/10.1038/s41598-017-13257-8
work_keys_str_mv AT tatsutayukina imagingmassspectrometryanalysisofubiquinollocalizationinthemousebrainfollowingshorttermadministration
AT kasaikazuaki imagingmassspectrometryanalysisofubiquinollocalizationinthemousebrainfollowingshorttermadministration
AT maruyamachitose imagingmassspectrometryanalysisofubiquinollocalizationinthemousebrainfollowingshorttermadministration
AT hamanoyoshimitsu imagingmassspectrometryanalysisofubiquinollocalizationinthemousebrainfollowingshorttermadministration
AT matsuokazuhiko imagingmassspectrometryanalysisofubiquinollocalizationinthemousebrainfollowingshorttermadministration
AT katanohajime imagingmassspectrometryanalysisofubiquinollocalizationinthemousebrainfollowingshorttermadministration
AT tairashu imagingmassspectrometryanalysisofubiquinollocalizationinthemousebrainfollowingshorttermadministration

Ejemplares similares